prikaz prve stranice dokumenta Recombinant expression, purification and characterisation of two biomass degrading enzymes in Trichoderma reesei
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master's thesis
Recombinant expression, purification and characterisation of two biomass degrading enzymes in Trichoderma reesei
Zagreb: University of Zagreb, Faculty of Food Technology and Biotechnology, 2018. urn:nbn:hr:159:710407
Požgajčić, Alen
University of Zagreb
Faculty of Food Technology and Biotechnology
Department of Biochemical Engineering
Laboratory for Biochemical Engineering, Industrial Microbiology and Malting and Brewing Technology

Institutional repository: Repository of the Faculty of Food Technology and Biotechnology

Cite this document

Požgajčić, A. (2018). Recombinant expression, purification and characterisation of two biomass degrading enzymes in Trichoderma reesei (Master's thesis). Zagreb: University of Zagreb, Faculty of Food Technology and Biotechnology. Retrieved from https://urn.nsk.hr/urn:nbn:hr:159:710407

Požgajčić, Alen. "Recombinant expression, purification and characterisation of two biomass degrading enzymes in Trichoderma reesei." Master's thesis, University of Zagreb, Faculty of Food Technology and Biotechnology, 2018. https://urn.nsk.hr/urn:nbn:hr:159:710407

Požgajčić, Alen. "Recombinant expression, purification and characterisation of two biomass degrading enzymes in Trichoderma reesei." Master's thesis, University of Zagreb, Faculty of Food Technology and Biotechnology, 2018. https://urn.nsk.hr/urn:nbn:hr:159:710407

Požgajčić, A. (2018). 'Recombinant expression, purification and characterisation of two biomass degrading enzymes in Trichoderma reesei', Master's thesis, University of Zagreb, Faculty of Food Technology and Biotechnology, accessed 13 January 2025, https://urn.nsk.hr/urn:nbn:hr:159:710407

Požgajčić A. Recombinant expression, purification and characterisation of two biomass degrading enzymes in Trichoderma reesei [Master's thesis]. Zagreb: University of Zagreb, Faculty of Food Technology and Biotechnology; 2018 [cited 2025 January 13] Available at: https://urn.nsk.hr/urn:nbn:hr:159:710407

A. Požgajčić, "Recombinant expression, purification and characterisation of two biomass degrading enzymes in Trichoderma reesei", Master's thesis, University of Zagreb, Faculty of Food Technology and Biotechnology, Zagreb, 2018. Available at: https://urn.nsk.hr/urn:nbn:hr:159:710407

Metadata
TitleRecombinant expression, purification and characterisation of two biomass degrading enzymes in Trichoderma reesei
Title (croatian)Rekombinantna ekspresija, pročišćavanje i karakterizacija dvaju enzima za razgradnju biomase u Trichoderma reesei
AuthorAlen Požgajčić
MentorBožidar Šantek (mentor)
GranterUniversity of Zagreb
Faculty of Food Technology and Biotechnology
(Department of Biochemical Engineering)
(Laboratory for Biochemical Engineering, Industrial Microbiology and Malting and Brewing Technology)
Zagreb
Defense date and country2018-11-26, Croatia
Scientific / art field,
discipline and subdiscipline		BIOTECHNICAL SCIENCES
Biotechnology
Abstract
Plijesni, kao saptrofitski mikroorganizmi, igraju glavnu ulogu u zemljinom ciklusu ugljika na način da njihova enzimatska mašinerija rastavlja biljnu biomasu na jednostavne šećere. Kao primjer dva takva enzima, litička polisahardina monooksigenaza (LPMO) i celobioza dehidrogenaza (CDH) pokazuju sinergističku aktivnost. Njihova aktivnost pokazuje visoki potencijal za saharifikaciju biomase i proizvodnju biogoriva na industrijskoj razini. U ovom radu, CDH iz plijesni Neurospora crassa je ekspresioniran u plijesni Trichoderma reesei, pročišćen i karakterizirani su mu kinetički parametri zajedno s pH i temperaturnim optimumom. Istovremeno, inhibitori LPMOa su karakterizirani kako bi se objasnili mehanizmi inhibicije u prirodi.
Abstract (english)
Fungi, as a saprophytic microorganisms, play a central role in the terrestrial carbon cycle in a way that their enzymatic machineries deconstructs plant biomass to simple sugars. Two of the enzymes from that machinery, lytic polysaccharide monooxygenase (LPMO) and cellobiose dehydrogenase (CDH) have shown synergistic activity. Their activity shows high potential for industrial scale saccharification of biomass and production of biofuels. In this work, CDH from fungus Neurospora crassa has been expressed in fungus Trichoderma reesei, purified and characterised its kinetical parameters together with pH and temperature optima. Simultaneously, characterisation of LPMO inhibitors have been done so that we can explain the mechanisms of inhibition in the nature.
Keywords
Keywords (english)
Languageenglish
URN:NBNurn:nbn:hr:159:710407
Study programmeTitle: Bioprocess Engineering
Study programme type: university
Study level: graduate
Academic / professional title: magistar/magistra inženjer/inženjerka bioprocesnog inženjerstva (magistar/magistra inženjer/inženjerka bioprocesnog inženjerstva)
Type of resourceText
File originBorn digital
Access conditionsAccess restricted to students and staff of home institution
Terms of useLicence In copyright icon
Created on2019-11-27 11:37:33