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master's thesis
Usporedba aktivacije urođenog imunosnog odgovora u stanicama monocita i mišića čovjeka nakon infekcije adenovirusima tipa 5, 26 i 35
Zagreb: University of Zagreb, Faculty of Food Technology and Biotechnology, 2018. urn:nbn:hr:159:959265
Radić, Ivana
University of Zagreb
Faculty of Food Technology and Biotechnology
Department of Biochemical Engineering
Laboratory for Biology and Microbial Genetics

Institutional repository: Repository of the Faculty of Food Technology and Biotechnology

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Radić, I. (2018). Usporedba aktivacije urođenog imunosnog odgovora u stanicama monocita i mišića čovjeka nakon infekcije adenovirusima tipa 5, 26 i 35 (Master's thesis). Zagreb: University of Zagreb, Faculty of Food Technology and Biotechnology. Retrieved from https://urn.nsk.hr/urn:nbn:hr:159:959265

Radić, Ivana. "Usporedba aktivacije urođenog imunosnog odgovora u stanicama monocita i mišića čovjeka nakon infekcije adenovirusima tipa 5, 26 i 35." Master's thesis, University of Zagreb, Faculty of Food Technology and Biotechnology, 2018. https://urn.nsk.hr/urn:nbn:hr:159:959265

Radić, Ivana. "Usporedba aktivacije urođenog imunosnog odgovora u stanicama monocita i mišića čovjeka nakon infekcije adenovirusima tipa 5, 26 i 35." Master's thesis, University of Zagreb, Faculty of Food Technology and Biotechnology, 2018. https://urn.nsk.hr/urn:nbn:hr:159:959265

Radić, I. (2018). 'Usporedba aktivacije urođenog imunosnog odgovora u stanicama monocita i mišića čovjeka nakon infekcije adenovirusima tipa 5, 26 i 35', Master's thesis, University of Zagreb, Faculty of Food Technology and Biotechnology, accessed 09 November 2024, https://urn.nsk.hr/urn:nbn:hr:159:959265

Radić I. Usporedba aktivacije urođenog imunosnog odgovora u stanicama monocita i mišića čovjeka nakon infekcije adenovirusima tipa 5, 26 i 35 [Master's thesis]. Zagreb: University of Zagreb, Faculty of Food Technology and Biotechnology; 2018 [cited 2024 November 09] Available at: https://urn.nsk.hr/urn:nbn:hr:159:959265

I. Radić, "Usporedba aktivacije urođenog imunosnog odgovora u stanicama monocita i mišića čovjeka nakon infekcije adenovirusima tipa 5, 26 i 35", Master's thesis, University of Zagreb, Faculty of Food Technology and Biotechnology, Zagreb, 2018. Available at: https://urn.nsk.hr/urn:nbn:hr:159:959265

Metadata
TitleUsporedba aktivacije urođenog imunosnog odgovora u stanicama monocita i mišića čovjeka nakon infekcije adenovirusima tipa 5, 26 i 35
Title (english)The comparison of the induction of innate immune response in human monocytes and muscle cells after infection with the adenovirus type 5, 26 and 35
AuthorIvana Radić
MentorAndreja Ambriović Ristov (mentor)
GranterUniversity of Zagreb
Faculty of Food Technology and Biotechnology
(Department of Biochemical Engineering)
(Laboratory for Biology and Microbial Genetics)
Zagreb
Defense date and country2018-07-20, Croatia
Scientific / art field,
discipline and subdiscipline		BIOTECHNICAL SCIENCES
Biotechnology
Abstract
Adenovirus tipa 5 (AdV5) najkorišteniji je viralni vektor u genskoj terapiji pri čemu je jedan od nedostataka njegova velika seroprevalencija u populaciji što smanjuje mogućnost njegove uporabe te imunogenost vektora. Iz tog razloga, priseže se korištenju vektora koji u populaciji imaju nisku seroprevalenciju, kao što su adenovirus tipa 26 (AdV26) i adenovirus tipa 35 (AdV35). Urođeni imunosni odgovor čini prvu liniju obrane organizma od patogena, pa tako i adenovirusa. Infekcija adenovirusima potiče proizvodnju brojnih citokina i kemokina koji djeluju protuupalno te eliminiraju strane antigene. Adenovirusi različitih serotipova izazivaju urođeni imunosni odgovor različitog intenziteta. U ovom radu uspoređena je aktivacija urođenog imunosnog odgovora u stanicama monocita i mišića čovjeka nakon infekcije AdV5, AdV26 i AdV35. Uočeno je da AdV35 u stanicama monocita i mišića čovjeka izaziva najsnažniji urođeni imunosni odgovor i to poticanjem ekspresije gena koji kodiraju za IL-6, IL-8, IL-1β i TNFα odnosno povećanim lučenjem IL-6 i IL-8. Urođeni imunosni odgovor koji AdV26 potiče u RD i THP-1 stanicama sličan je odgovoru koji potiče infekcija s AdV5. Poticanje urođenog imunosnog odgovora infekcijom AdV5, AdV26 i AdV35 posredovano je s pIRF-3 i NF-κB. Ovi rezultati upućuju na potencijal AdV26 i AdV35 kao povoljnih vektora za prijenos gena i vakcinaciju.
Abstract (english)
Adenovirus type 5 (AdV5) is the most widely used viral vector in gene therapy, however its immunogenicity and high seroprevalence in the population reduces the possibility of usage of this vector. For those reasons, vectors with low seroprevalence in the population such as adenovirus type 26 (AdV26) and adenovirus type 35 (AdV35), are used as an alternative approach. The innate immune response is the first line of defense of the organism from pathogens, thus also adenoviruses. Adenoviral infection triggers the production of numerous anti-inflammatory cytokines and chemokines that eliminate foreign antigens. Different serotypes of adenoviruses cause innate immune responses of varying intensity. This thesis compares the induction of innate immune responses in human monocytes and muscle cells after AdV5, AdV26 and AdV35 infection. It was observed that AdV35 in human monocytes and muscle cells induces the most powerful innate immune response by stimulating expression of genes encoding IL-6, IL-8, IL-1β and TNFα, thus increasing IL-6 and IL-8 secretion. The innate immune response triggered by AdV26 in human monocytes and muscle cells is similar to the response triggered by AdV5 infection. The induction of the innate immune response by AdV5, AdV26 and AdV35 infection is mediated by pIRF-3 and NF-κB. These findings point to the potential of AdV26 and AdV35 as favorable vectors for gene transfer and vaccination.
Keywords
Keywords (english)
Languagecroatian
URN:NBNurn:nbn:hr:159:959265
Study programmeTitle: Molecular Biotechnology
Study programme type: university
Study level: graduate
Academic / professional title: magistar/magistra inženjer/inženjerka molekularne biotehnologije (magistar/magistra inženjer/inženjerka molekularne biotehnologije)
Type of resourceText
File originBorn digital
Access conditionsOpen access
Terms of useLicence In copyright icon
Created on2019-11-28 08:00:56