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master's thesis
Primjena različitih nosača za mikroinkapsulaciju bakterijskih sojeva Lactobacillus plantarum D13 i Lactococcus lactis ZG7-10
Zagreb: University of Zagreb, Faculty of Food Technology and Biotechnology, 2018. urn:nbn:hr:159:896563
Nušinović, Emina
University of Zagreb
Faculty of Food Technology and Biotechnology
Department of Biochemical Engineering
Laboratory for Antibiotic, Enzyme, Probiotic and Starter Cultures Technology

Institutional repository: Repository of the Faculty of Food Technology and Biotechnology

Cite this document

Nušinović, E. (2018). Primjena različitih nosača za mikroinkapsulaciju bakterijskih sojeva Lactobacillus plantarum D13 i Lactococcus lactis ZG7-10 (Master's thesis). Zagreb: University of Zagreb, Faculty of Food Technology and Biotechnology. Retrieved from https://urn.nsk.hr/urn:nbn:hr:159:896563

Nušinović, Emina. "Primjena različitih nosača za mikroinkapsulaciju bakterijskih sojeva Lactobacillus plantarum D13 i Lactococcus lactis ZG7-10." Master's thesis, University of Zagreb, Faculty of Food Technology and Biotechnology, 2018. https://urn.nsk.hr/urn:nbn:hr:159:896563

Nušinović, Emina. "Primjena različitih nosača za mikroinkapsulaciju bakterijskih sojeva Lactobacillus plantarum D13 i Lactococcus lactis ZG7-10." Master's thesis, University of Zagreb, Faculty of Food Technology and Biotechnology, 2018. https://urn.nsk.hr/urn:nbn:hr:159:896563

Nušinović, E. (2018). 'Primjena različitih nosača za mikroinkapsulaciju bakterijskih sojeva Lactobacillus plantarum D13 i Lactococcus lactis ZG7-10', Master's thesis, University of Zagreb, Faculty of Food Technology and Biotechnology, accessed 27 August 2024, https://urn.nsk.hr/urn:nbn:hr:159:896563

Nušinović E. Primjena različitih nosača za mikroinkapsulaciju bakterijskih sojeva Lactobacillus plantarum D13 i Lactococcus lactis ZG7-10 [Master's thesis]. Zagreb: University of Zagreb, Faculty of Food Technology and Biotechnology; 2018 [cited 2024 August 27] Available at: https://urn.nsk.hr/urn:nbn:hr:159:896563

E. Nušinović, "Primjena različitih nosača za mikroinkapsulaciju bakterijskih sojeva Lactobacillus plantarum D13 i Lactococcus lactis ZG7-10", Master's thesis, University of Zagreb, Faculty of Food Technology and Biotechnology, Zagreb, 2018. Available at: https://urn.nsk.hr/urn:nbn:hr:159:896563

Metadata
TitlePrimjena različitih nosača za mikroinkapsulaciju bakterijskih sojeva Lactobacillus plantarum D13 i Lactococcus lactis ZG7-10
Title (english)Application of different carriers for microencapsulation of bacterial strains Lactobacillus plantarum D13 AND Lactococcus lactis ZG7-10
AuthorEmina Nušinović
MentorBlaženka Kos (mentor)
GranterUniversity of Zagreb
Faculty of Food Technology and Biotechnology
(Department of Biochemical Engineering)
(Laboratory for Antibiotic, Enzyme, Probiotic and Starter Cultures Technology)
Zagreb
Defense date and country2018-04-06, Croatia
Scientific / art field,
discipline and subdiscipline		BIOTECHNICAL SCIENCES
Biotechnology
Abstract
Da bi probiotički pripravci pokazali pozitivne učinke na zdravlje potrošača, moraju sadržavati visoku koncentraciju živih stanica. Zbog toga se istražuju različiti matriksi i metode kako bi se povećalo njihovo preživljavanje tijekom proizvodnje, čuvanja i primjene u različitim prehrambenim proizvodima te nakon oralne primjene u gastrointestinalnom traktu domaćina. Cilj ovog rada bio je ispitati utjecaj mikroinkapsulacije na preživljavanje bakterija Lactobacillus plantarum D13 i Lactococcus lactis ZG7-10. Stoga je provedena mikroinkapsulacija primjenom tri nosača, alginata, κ-karagenana i kazeina uz djelovanje transglutaminaze, a zatim i liofilizacija mikroinkapsuliranih stanica uz upotrebu različitih lioprotektora. Mikroinkapsulacija u alginatu i dodatak obranog mlijeka kao lioprotektora, pokazali su se najučinkovitiji za oba soja. Prolaskom mikroinkapsuliranih liofiliziranih stanica kroz simulirane uvjete gastrointestinalnog trakta, visok postotak preživjelih stanica ukazuje na zaštitni učinak mikroinkapsulacije za probiotičke sojeve, pri čemu je broj živih stanica bio veći od 108CFU/g, što je u skladu sa zahtjevima koje moraju ispunjavati probiotički proizvodi.
Abstract (english)
In order to express their benefits on human health, the probiotic preparations need to contain high concentration of viable cells. Therefore, different matrices and methods have been investigated to improve their survival during production, storage or application in different food products, and after an oral application in the host gastrointestinal conditions. The aim of this thesis was to investigate influence of microencapsulation on the survival of probiotic bacteria Lactobacillus plantarum D13 and Lactococcus lactis ZG7-10. Therefore, the microencapsulation was performed with application of three different carriers, alginate, κ –carrageenan and transglutaminase-induced casein, and then lyophilization of microencapsulated cells with the addition of various lyoprotectors. Microencapsulation in alginate, and addition of skim milk as lyoprotector, proved to be most effective for both bacterial strains. After transit through simulated gastrointestinal tract conditions, high percentage of viable cells indicates the protective effect of the microencapsulation on the probiotic strains, wherein the number of viable cells was above 108 CFU/g, what is in accordance with the claims set up for probiotic products.
Keywords
Keywords (english)
Languagecroatian
URN:NBNurn:nbn:hr:159:896563
Study programmeTitle: Bioprocess Engineering
Study programme type: university
Study level: graduate
Academic / professional title: magistar/magistra inženjer/inženjerka bioprocesnog inženjerstva (magistar/magistra inženjer/inženjerka bioprocesnog inženjerstva)
Type of resourceText
File originBorn digital
Access conditionsOpen access
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Created on2019-11-28 10:10:38