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master's thesis
Genotoksični učinak irinotekana na ljudske tumorske stanice jetre i crijeva
Zagreb: University of Zagreb, Faculty of Food Technology and Biotechnology, 2018. urn:nbn:hr:159:887469
Joukhadar, Lejla
University of Zagreb
Faculty of Food Technology and Biotechnology
Department of Biochemical Engineering
Laboratory for Biology and Microbial Genetics

Institutional repository: Repository of the Faculty of Food Technology and Biotechnology

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Joukhadar, L. (2018). Genotoksični učinak irinotekana na ljudske tumorske stanice jetre i crijeva (Master's thesis). Zagreb: University of Zagreb, Faculty of Food Technology and Biotechnology. Retrieved from https://urn.nsk.hr/urn:nbn:hr:159:887469

Joukhadar, Lejla. "Genotoksični učinak irinotekana na ljudske tumorske stanice jetre i crijeva." Master's thesis, University of Zagreb, Faculty of Food Technology and Biotechnology, 2018. https://urn.nsk.hr/urn:nbn:hr:159:887469

Joukhadar, Lejla. "Genotoksični učinak irinotekana na ljudske tumorske stanice jetre i crijeva." Master's thesis, University of Zagreb, Faculty of Food Technology and Biotechnology, 2018. https://urn.nsk.hr/urn:nbn:hr:159:887469

Joukhadar, L. (2018). 'Genotoksični učinak irinotekana na ljudske tumorske stanice jetre i crijeva', Master's thesis, University of Zagreb, Faculty of Food Technology and Biotechnology, accessed 30 December 2024, https://urn.nsk.hr/urn:nbn:hr:159:887469

Joukhadar L. Genotoksični učinak irinotekana na ljudske tumorske stanice jetre i crijeva [Master's thesis]. Zagreb: University of Zagreb, Faculty of Food Technology and Biotechnology; 2018 [cited 2024 December 30] Available at: https://urn.nsk.hr/urn:nbn:hr:159:887469

L. Joukhadar, "Genotoksični učinak irinotekana na ljudske tumorske stanice jetre i crijeva", Master's thesis, University of Zagreb, Faculty of Food Technology and Biotechnology, Zagreb, 2018. Available at: https://urn.nsk.hr/urn:nbn:hr:159:887469

Metadata
TitleGenotoksični učinak irinotekana na ljudske tumorske stanice jetre i crijeva
Title (english)Genotoxic effect of irinotecanon human liver and colon tumor cells
AuthorLejla Joukhadar
MentorKsenija Durgo (mentor)
GranterUniversity of Zagreb
Faculty of Food Technology and Biotechnology
(Department of Biochemical Engineering)
(Laboratory for Biology and Microbial Genetics)
Zagreb
Defense date and country2018-10-30, Croatia
Scientific / art field,
discipline and subdiscipline		BIOTECHNICAL SCIENCES
Food Technology
Abstract
Od ukupnog broja svih godišnje dijagnosticiranih karcinoma u svijetu, karcinomi probavnog sustava su na vodećoj poziciji. Među njima najvišu pojavnost ima kolorektalni karcinom, što ga svrstava među vodeće zdravstvene probleme kako u svijetu tako i u Hrvatskoj. Irinotekan (CPT-11) je polusintetski analog kamptotecina (CPT) koji je glavni lijek izbora u terapiji metastatskog kolorektalnog karcinoma. Cilj ovog rada je istražiti citotoksičnost, genotoksičnost i svojstvo indukcije slobodnih radikala različitih koncentracija irinotekana. Kao test sustavi korištene su humana stanična linija hepatocelularnog karcinoma jetre (HepG2) i humana stanična linija adenokarcinoma epitela debelog crijeva (Caco-2) koje su bile izložene djelovanju irinotekana tijekom 2, 4, 24 i 48 sati. Dokazano je da irinotekan djeluje toksično produljenjem vremena tretmana na obje stanične linije. Na stanicama jetre toksičnost raste u ovisnosti o koncentraciji, dok na stanicama crijeva ovisnost o kocentraciji nije dokazana. Također je uočena povezanost citotoksičnog učinka irinotekana s indukcijom slobodnih radikala. S produljenjem vremena inkubacije dolazi do genotoksičnog učinka irinotekana na obje tretirane stanične linije.
Abstract (english)
Of the total number of all yearly diagnosed carcinomas in the world, cancers that affect the digestive system are in a leading position. Among them, the highest incidence is colorectal cancer, which puts it among the leading health problems in the world as well as in Croatia. Irinotecan (CPT-11) is a semi-synthetic analogue of camptothecin (CPT) which is the major drug in the choice of metastatic colorectal cancer therapy. The aim of this work is to investigate the cytotoxicity, genotoxicity and induction of free radicals of different concentrations of irinotecan. As the test system the human hepatocellular carcinoma cell line (HepG2) and the human colon adenocarcinoma cell line (Caco-2) were exposed to irinotecan during 2, 4, 24, and 48 hours. Results have shown that irinotecan cytotoxicity is dependent on the cell line. In liver cells, toxicity is increasing depending on concentrations, while on the colon cells no dependence on the concentration has been demonstrated. Also, the association of cytotoxic effect of irinotecan with the induction of free radicals was observed. With the prolongation of incubation time, the genotoxic effect of irinotecan on both treated cell lines is reached.
Keywords
Keywords (english)
Languagecroatian
URN:NBNurn:nbn:hr:159:887469
Study programmeTitle: Food Safety Management
Study programme type: university
Study level: graduate
Academic / professional title: magistar/magistra inženjer/inženjerka upravljanja sigurnošću hrane (magistar/magistra inženjer/inženjerka upravljanja sigurnošću hrane)
Type of resourceText
File originBorn digital
Access conditionsOpen access
Terms of useLicence Attribution-NonCommercial-ShareAlike 3.0 Unported (CC BY-NC-SA 3.0) icon
Created on2019-12-04 11:10:20