prikaz prve stranice dokumenta Evaluacija učinaka resveratrola pri tretmanu stanica polikloriranim bifenilima određena in vitro metodama
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master's thesis
Evaluacija učinaka resveratrola pri tretmanu stanica polikloriranim bifenilima određena in vitro metodama
Zagreb: University of Zagreb, Faculty of Food Technology and Biotechnology, 2019. urn:nbn:hr:159:824104
Petković, Tea
University of Zagreb
Faculty of Food Technology and Biotechnology
Department of Chemistry and Biochemistry
Laboratory for Toxicology

Institutional repository: Repository of the Faculty of Food Technology and Biotechnology

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Petković, T. (2019). Evaluacija učinaka resveratrola pri tretmanu stanica polikloriranim bifenilima određena in vitro metodama (Master's thesis). Zagreb: University of Zagreb, Faculty of Food Technology and Biotechnology. Retrieved from https://urn.nsk.hr/urn:nbn:hr:159:824104

Petković, Tea. "Evaluacija učinaka resveratrola pri tretmanu stanica polikloriranim bifenilima određena in vitro metodama." Master's thesis, University of Zagreb, Faculty of Food Technology and Biotechnology, 2019. https://urn.nsk.hr/urn:nbn:hr:159:824104

Petković, Tea. "Evaluacija učinaka resveratrola pri tretmanu stanica polikloriranim bifenilima određena in vitro metodama." Master's thesis, University of Zagreb, Faculty of Food Technology and Biotechnology, 2019. https://urn.nsk.hr/urn:nbn:hr:159:824104

Petković, T. (2019). 'Evaluacija učinaka resveratrola pri tretmanu stanica polikloriranim bifenilima određena in vitro metodama', Master's thesis, University of Zagreb, Faculty of Food Technology and Biotechnology, accessed 16 November 2024, https://urn.nsk.hr/urn:nbn:hr:159:824104

Petković T. Evaluacija učinaka resveratrola pri tretmanu stanica polikloriranim bifenilima određena in vitro metodama [Master's thesis]. Zagreb: University of Zagreb, Faculty of Food Technology and Biotechnology; 2019 [cited 2024 November 16] Available at: https://urn.nsk.hr/urn:nbn:hr:159:824104

T. Petković, "Evaluacija učinaka resveratrola pri tretmanu stanica polikloriranim bifenilima određena in vitro metodama", Master's thesis, University of Zagreb, Faculty of Food Technology and Biotechnology, Zagreb, 2019. Available at: https://urn.nsk.hr/urn:nbn:hr:159:824104

Metadata
TitleEvaluacija učinaka resveratrola pri tretmanu stanica polikloriranim bifenilima određena in vitro metodama
Title (english)Evaluation of resveratrol effects on cells treated with polychlorinated biphenyls determined with in vitro methods
AuthorTea Petković
MentorIvana Kmetič (mentor)
GranterUniversity of Zagreb
Faculty of Food Technology and Biotechnology
(Department of Chemistry and Biochemistry)
(Laboratory for Toxicology)
Zagreb
Defense date and country2019-07-19, Croatia
Scientific / art field,
discipline and subdiscipline		BIOTECHNICAL SCIENCES
Nutrition
Abstract
U ovom radu ispitan je učinak različitih koncentracija resveratrola (2,5-20 μM) pri citotoksičnom djelovanju 75 μM PCB-a 77 ili 75 μM PCB-a 153 na CHO (Chinese Hamster Ovary) – K1 stanice metodama Neutral Red, Kenacid Blue te metodom protočne citometrije. Utvrđeno je statistički značajno smanjenje vijabilnosti stanica u odnosu na kontrolni uzorak pri tretmanu CHO-K1 stanica s PCB-em 77 i PCB-em 153 metodama Neutral Red i Kenacid Blue. Blagi protektivni učinak resveratrola (u koncentracijama 2,5, 5 i 10 μM) je primijećen pri citotoksičnom djelovanju PCB-a 153, no nije se pokazao statistički značajnim. Toksični učinci dioksinu sličnog kongenera-PCB-a 77 na stanice ovarija nisu umanjeni, čak suprotno - djelovanjem resveratrola izraženiji je učinak na pad vijabilnosti stanica. Sve primijenjene in vitro metode pokazale su korespondirajuće rezultate.
Abstract (english)
In this study, the effects of different resveratrol concentrations (2.5-20 μM) on cytotoxic activity of 75 μM PCB 77 or 75 μM PCB 153 on CHO (Chinese Hamster Ovary) - K1 cells were determined by Neutral Red method, Kenacid Blue method and by flow cytometry. Statistically significant reduction in cell viability was observed after treatment of CHO-K1 cells with PCB 77 or PCB 153 in comparison to the control sample determined with Neutral Red and Kenacid Blue methods. The slight protective effect of resveratrol (at concentrations of 2.5, 5 and 10 μM) on cytotoxic activity induced by PCB 153 was observed, but results were not statistically significant. Toxic effects of dioxin-like congener-PCB 77 on ovarian cells were not reduced by the action of resveratrol, on the contrary - resveratrol has shown a more pronounced effect on the reduction in cell viability. All applied in vitro methods have shown to be consistent within results.
Keywords
Keywords (english)
Languagecroatian
URN:NBNurn:nbn:hr:159:824104
Study programmeTitle: Nutrition
Study programme type: university
Study level: graduate
Academic / professional title: magistar/magistra nutricionizma (magistar/magistra nutricionizma)
Type of resourceText
File originBorn digital
Access conditionsOpen access
Terms of useLicence In copyright icon
Created on2019-12-05 11:36:27