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master's thesis
Biološka evaluacija ferocenskih peptidomimetika
Zagreb: University of Zagreb, Faculty of Food Technology and Biotechnology, 2019. urn:nbn:hr:159:320794
Mihalj, Nika
University of Zagreb
Faculty of Food Technology and Biotechnology
Department of Biochemical Engineering
Laboratory for Cell Technology, Application and Biotransformations

Institutional repository: Repository of the Faculty of Food Technology and Biotechnology

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Mihalj, N. (2019). Biološka evaluacija ferocenskih peptidomimetika (Master's thesis). Zagreb: University of Zagreb, Faculty of Food Technology and Biotechnology. Retrieved from https://urn.nsk.hr/urn:nbn:hr:159:320794

Mihalj, Nika. "Biološka evaluacija ferocenskih peptidomimetika." Master's thesis, University of Zagreb, Faculty of Food Technology and Biotechnology, 2019. https://urn.nsk.hr/urn:nbn:hr:159:320794

Mihalj, Nika. "Biološka evaluacija ferocenskih peptidomimetika." Master's thesis, University of Zagreb, Faculty of Food Technology and Biotechnology, 2019. https://urn.nsk.hr/urn:nbn:hr:159:320794

Mihalj, N. (2019). 'Biološka evaluacija ferocenskih peptidomimetika', Master's thesis, University of Zagreb, Faculty of Food Technology and Biotechnology, accessed 23 November 2024, https://urn.nsk.hr/urn:nbn:hr:159:320794

Mihalj N. Biološka evaluacija ferocenskih peptidomimetika [Master's thesis]. Zagreb: University of Zagreb, Faculty of Food Technology and Biotechnology; 2019 [cited 2024 November 23] Available at: https://urn.nsk.hr/urn:nbn:hr:159:320794

N. Mihalj, "Biološka evaluacija ferocenskih peptidomimetika", Master's thesis, University of Zagreb, Faculty of Food Technology and Biotechnology, Zagreb, 2019. Available at: https://urn.nsk.hr/urn:nbn:hr:159:320794

Metadata
TitleBiološka evaluacija ferocenskih peptidomimetika
Title (english)Biological evaluation of ferrocene peptidomimetics
AuthorNika Mihalj
MentorKristina Radošević (mentor)
GranterUniversity of Zagreb
Faculty of Food Technology and Biotechnology
(Department of Biochemical Engineering)
(Laboratory for Cell Technology, Application and Biotransformations)
Zagreb
Defense date and country2019-07-19, Croatia
Scientific / art field,
discipline and subdiscipline		BIOTECHNICAL SCIENCES
Biotechnology
Abstract
Peptidomimetici su spojevi čiji esencijalni farmakoforni elementi oponašaju 3D strukturu prirodnog peptida i/ili proteina te zadržavaju njegovu sposobnost interakcije s biološkim ciljanim sustavima, pri čemu zadržavaju jednak ili unaprijeđen biološki učinak. Budući da su peptidomimetici poznati kao potencijalni antitumorski agensi, u ovom radu je ispitana in vitro biološka aktivnost četiri različita ferocenska peptidomimetika na humanim tumorskim staničnim linijama (HeLa i MCF-7) te je određen mehanizam njihovog citotoksičnog djelovanja protočnom citometrijom. Sva četiri ispitana ferocenska peptidomimetika pokazala su inhibitorni učinak na rast staničnih linija, pri čemu peptidomimetik 1 ima najveći inhibitorni učinak na proliferaciju stanica. Također, određivanjem tipa stanične smrti u uzorcima tretiranim ispitivanim spojevima primjenom protočne citometrije je pokazano da peptidomimetik 1 ima najveći potencijal za induciranje apoptotične smrti stanica od svih ispitivanih peptidomimetika. Dobiveni rezultati ukazuju na mogući antitumorski učinak ispitivanih ferocenskih peptidomimetika.
Abstract (english)
Peptidomimetics are compounds whose essential pharmacophore elements mimic a natural peptide or protein in 3D space and which retain the ability to interact with the biological target and produce the same or even pronounced biological effect. They are known to be potential antitumor agents so in this research, in vitro biological activity of four different ferrocene peptidomimetics on human tumor cell lines (HeLa and MCF-7) and the mechanism of their cytotoxic activity using flow cytometry were determined. Based on the obtained results, their impact on people could be determined. All tested peptidomimetics showed an inhibitory effect on cell lines growth and peptidomimetic 1 showed the greatest inhibitory effect on cell lines proliferation. The results have also shown that the greatest inductor of apoptotic cell death is peptidomimetic 1. The obtained results indicate that these ferrocene peptidomimetics could have a possible antitumor effect.
Keywords
Keywords (english)
Languagecroatian
URN:NBNurn:nbn:hr:159:320794
Study programmeTitle: Molecular Biotechnology
Study programme type: university
Study level: graduate
Academic / professional title: magistar/magistra inženjer/inženjerka molekularne biotehnologije (magistar/magistra inženjer/inženjerka molekularne biotehnologije)
Type of resourceText
File originBorn digital
Access conditionsOpen access
Terms of useLicence In copyright icon
Created on2019-12-05 11:41:45