prikaz prve stranice dokumenta Određivanje rezidua tetraciklina u jajima primjenom LC-MS/MS i UHPLC/Q-TOF-MS metode
Access restricted to students and staff of home institution
master's thesis
Određivanje rezidua tetraciklina u jajima primjenom LC-MS/MS i UHPLC/Q-TOF-MS metode
Zagreb: University of Zagreb, Faculty of Food Technology and Biotechnology, 2019. urn:nbn:hr:159:784752
Čuljak, Nina
University of Zagreb
Faculty of Food Technology and Biotechnology
Department of Biochemical Engineering
Laboratory for Antibiotic, Enzyme, Probiotic and Starter Cultures Technology

Institutional repository: Repository of the Faculty of Food Technology and Biotechnology

Cite this document

Čuljak, N. (2019). Određivanje rezidua tetraciklina u jajima primjenom LC-MS/MS i UHPLC/Q-TOF-MS metode (Master's thesis). Zagreb: University of Zagreb, Faculty of Food Technology and Biotechnology. Retrieved from https://urn.nsk.hr/urn:nbn:hr:159:784752

Čuljak, Nina. "Određivanje rezidua tetraciklina u jajima primjenom LC-MS/MS i UHPLC/Q-TOF-MS metode." Master's thesis, University of Zagreb, Faculty of Food Technology and Biotechnology, 2019. https://urn.nsk.hr/urn:nbn:hr:159:784752

Čuljak, Nina. "Određivanje rezidua tetraciklina u jajima primjenom LC-MS/MS i UHPLC/Q-TOF-MS metode." Master's thesis, University of Zagreb, Faculty of Food Technology and Biotechnology, 2019. https://urn.nsk.hr/urn:nbn:hr:159:784752

Čuljak, N. (2019). 'Određivanje rezidua tetraciklina u jajima primjenom LC-MS/MS i UHPLC/Q-TOF-MS metode', Master's thesis, University of Zagreb, Faculty of Food Technology and Biotechnology, accessed 23 May 2024, https://urn.nsk.hr/urn:nbn:hr:159:784752

Čuljak N. Određivanje rezidua tetraciklina u jajima primjenom LC-MS/MS i UHPLC/Q-TOF-MS metode [Master's thesis]. Zagreb: University of Zagreb, Faculty of Food Technology and Biotechnology; 2019 [cited 2024 May 23] Available at: https://urn.nsk.hr/urn:nbn:hr:159:784752

N. Čuljak, "Određivanje rezidua tetraciklina u jajima primjenom LC-MS/MS i UHPLC/Q-TOF-MS metode", Master's thesis, University of Zagreb, Faculty of Food Technology and Biotechnology, Zagreb, 2019. Available at: https://urn.nsk.hr/urn:nbn:hr:159:784752

Metadata
TitleOdređivanje rezidua tetraciklina u jajima primjenom LC-MS/MS i UHPLC/Q-TOF-MS metode
Title (english)Determination of tetracycline residues in eggs using LC-MS/MS and UHPLC/Q-TOF-MS methods
AuthorNina Čuljak
MentorBlaženka Kos (mentor)
GranterUniversity of Zagreb
Faculty of Food Technology and Biotechnology
(Department of Biochemical Engineering)
(Laboratory for Antibiotic, Enzyme, Probiotic and Starter Cultures Technology)
Zagreb
Defense date and country2019-07-10, Croatia
Scientific / art field,
discipline and subdiscipline		BIOTECHNICAL SCIENCES
Biotechnology
Abstract
Upotreba antibiotika kao veterinarskih lijekova može dovesti do pojave njihovih rezidua u hrani životinjskog podrijetla kao što su meso goveda, svinja i peradi, mlijeko i mliječni proizvodi, jaja, ribe i med. Zahvaljujući širokom spektru djelovanja prema Gram-pozitivnim i Gram-negativnim bakterijama, rikecijama, mikoplazmama, klamidijama i jednostaničnim parazitima, tetraciklinski antibiotici su našli široku primjenu u veterinarskoj medicini. Rezidui antibiotika mogu imati nepovoljne učinke na zdravlje potrošača, a jedna od posljedica pretjerane uporabe antibiotika je i širenje antibiotičke rezistencije. Stoga je nužno razviti učinkovite metode za određivanje rezidua tetraciklinskih antibiotika u hrani životinjskog podrijetla kako bi se spriječilo daljnje širenje antibiotičke rezistencije. U ovom je radu provedena optimizacija tekućinske kromatografije s tandemskom spektrometrijom masa (LC-MS/MS) i tekućinske kromatografije ultravisoke djelotvornosti sa spektrometrijom masa i analizatorom vremena preleta (UHPLC/Q-TOF-MS), u svrhu postizanja maksimalne osjetljivosti i selektivnosti instrumenata za određivanje rezidua tetraciklina, epitetraciklina, oksitetraciklina, epioksitetraciklina, klortetraciklina, epiklortetraciklina, i doksiciklina, u obogaćenim uzorcima jaja, pri čemu je veća osjetljivost postignuta UHPLC/Q-TOF-MS metodom. Usporedbom rezultata određivanja rezidua tetraciklinskih antibiotika u uzorcima jaja pokazalo se da su obje primjenjene metode ekstrakcije analita, otapalom i Quechers ekstrakcijom, uspješno provedene, pri čemu je Quechers ekstrakcijom, primjenom LC-MS/MS metode, postignuta bolja ponovljivost, nego kod uzoraka dobivenih ekstrakcijom organskim otapalom.
Abstract (english)
The usage of antibiotics as veterinary drugs can lead to apperance of residues in foods of animal origin, including meat from bovine and porcine animals and poultry, milk and dairy products, eggs, fish and honey. Due to broad spectrum of activity against Gram-positive and Gram-negative bacteria, rickettsiae, mycoplasmas, chlamydiae and protosoan parasites, tetracyclines are wildely used in veterinary medicine. Antibiotic residues can have negative effects on health of consumer who was in contact with contaminated food, and one consequence of excessive usage of antibiotics is antibiotic resistance. Therefore, it is necessery to develop effective methods for determination residues of tetracyclines in animal-derived foods, in order to prevent further spread of antibiotic resistance. In this paper optimization of liquid chromatography with tandem mass spectrometry (LC-MS/MS) and ultra-high performance liquid chromatography coupled with hybrid quadrupole time-of-flight mass spectrometry (UHPLC/Q-TOF-MS) methods was carried out in order to achieve maximum instrument sensitivity and selectivity for determining residues of tetracycline, epitetracycline, oxytetracycline, epioxytetracycline, chlortetracycline, epichlortetracycline and doxycycline, in enriched egg samples, where UHPLC/Q-TOF-MS method had shown higher sensitivity. Both extraction methods, Quechers extraction and organic solvent extraction, were shown to be efficient. However, compared to samples obtained with organic solvent extraction, samples obtained with Quechers extraction, using LC-MS/MS method, achieved better repeatability.
Keywords
Keywords (english)
Languagecroatian
URN:NBNurn:nbn:hr:159:784752
Study programmeTitle: Molecular Biotechnology
Study programme type: university
Study level: graduate
Academic / professional title: magistar/magistra inženjer/inženjerka molekularne biotehnologije (magistar/magistra inženjer/inženjerka molekularne biotehnologije)
Type of resourceText
File originBorn digital
Access conditionsAccess restricted to students and staff of home institution
Terms of useLicence In copyright icon
Created on2019-12-06 12:17:08