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master's thesis
Detekcija i karakterizacija trim mobilnih elemenata u vrstama školjkaša cerastoderma edule i Glycymeris glycymeris
Zagreb: University of Zagreb, Faculty of Food Technology and Biotechnology, 2019. urn:nbn:hr:159:711303
Cedilak, Andrea
University of Zagreb
Faculty of Food Technology and Biotechnology
Department of Biochemical Engineering
Laboratory for Biology and Microbial Genetics

Institutional repository: Repository of the Faculty of Food Technology and Biotechnology

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Cedilak, A. (2019). Detekcija i karakterizacija trim mobilnih elemenata u vrstama školjkaša cerastoderma edule i Glycymeris glycymeris (Master's thesis). Zagreb: University of Zagreb, Faculty of Food Technology and Biotechnology. Retrieved from https://urn.nsk.hr/urn:nbn:hr:159:711303

Cedilak, Andrea. "Detekcija i karakterizacija trim mobilnih elemenata u vrstama školjkaša cerastoderma edule i Glycymeris glycymeris." Master's thesis, University of Zagreb, Faculty of Food Technology and Biotechnology, 2019. https://urn.nsk.hr/urn:nbn:hr:159:711303

Cedilak, Andrea. "Detekcija i karakterizacija trim mobilnih elemenata u vrstama školjkaša cerastoderma edule i Glycymeris glycymeris." Master's thesis, University of Zagreb, Faculty of Food Technology and Biotechnology, 2019. https://urn.nsk.hr/urn:nbn:hr:159:711303

Cedilak, A. (2019). 'Detekcija i karakterizacija trim mobilnih elemenata u vrstama školjkaša cerastoderma edule i Glycymeris glycymeris', Master's thesis, University of Zagreb, Faculty of Food Technology and Biotechnology, accessed 03 December 2024, https://urn.nsk.hr/urn:nbn:hr:159:711303

Cedilak A. Detekcija i karakterizacija trim mobilnih elemenata u vrstama školjkaša cerastoderma edule i Glycymeris glycymeris [Master's thesis]. Zagreb: University of Zagreb, Faculty of Food Technology and Biotechnology; 2019 [cited 2024 December 03] Available at: https://urn.nsk.hr/urn:nbn:hr:159:711303

A. Cedilak, "Detekcija i karakterizacija trim mobilnih elemenata u vrstama školjkaša cerastoderma edule i Glycymeris glycymeris", Master's thesis, University of Zagreb, Faculty of Food Technology and Biotechnology, Zagreb, 2019. Available at: https://urn.nsk.hr/urn:nbn:hr:159:711303

Metadata
TitleDetekcija i karakterizacija trim mobilnih elemenata u vrstama školjkaša cerastoderma edule i Glycymeris glycymeris
Title (english)Detection and characterization of trim mobile elements in bivalve SPECIES Cerastoderma edule and Glycymeris glycymeris
AuthorAndrea Cedilak
MentorIvan-Krešimir Svetec (mentor)
GranterUniversity of Zagreb
Faculty of Food Technology and Biotechnology
(Department of Biochemical Engineering)
(Laboratory for Biology and Microbial Genetics)
Zagreb
Defense date and country2019-01-25, Croatia
Scientific / art field,
discipline and subdiscipline		BIOTECHNICAL SCIENCES
Interdisciplinary Technical Sciences
Abstract
TRIM su mobilni elementi koji spadaju u skupinu neautonomnih retrotranspozona i do sada su dokazani u iznimno malom broju animalnih genoma. Školjkaši su skupina organizama još uvijek slabo okarakterizirana na genomskoj razini, no u najnovije vrijeme postali su predmetom istraţivanja jer je prepoznata njihova gospodarska i ekološka vaţnost. U sklopu ovog rada provedena su istraţivanja u svrhu detekcije i karakterizacije mobilnih elemenata TRIM u vrstama školjkaša Cerastoderma edule i Glycymeris glycymeris te njihova usporedba s elementima iz drugih vrsta školjkaša koji sadrţe ove retroelemente. Postojanje elemenata dokazano je analizom otisaka kolonija, odnosno bioinformatičkom analizom sekvenciranih uzoraka. Dobiven je postotak sličnosti iznad 90% unutar vrste, odnosno 75% izmeĎu ove dvije vrste. U drugom djelu ovog rada po prvi put je odreĎivan udio TRIM elemenata u genomu za svih 11 vrsta korištenih u ovom radu te je kapljičnom analizom dobiveno da kod svih vrsta iznosi <1% genoma.
Abstract (english)
TRIM mobile elements belong to the group of non-autonomous retrotransposons whose presence has been observed in very few animal genomes. Bivalves are a group of organisms still poorly explored at the genome level that have recently become the research object of interest due to their economic and ecological importance. Within this work, detection and characterization of TRIM mobile elements in the bivalve species Cerastoderma edule and Glycymeris glycymeris was carried out, followed by the comparison with elements from other bivalve species containing these retroelements. The existence of elements has been proved with the colony lift, actually with bioinformatics analysis of sequenced samples. There is a percentage of similarity above 90% within the species, or 75% between these two species. In the second part of this paper, for the first time, a determined fraction of the TRIM elements in the genome for all 11 species used in this paper was obtained with dot blot analysis and it was obtained that in all species is <1% of the genome.
Keywords
Keywords (english)
Languagecroatian
URN:NBNurn:nbn:hr:159:711303
Study programmeTitle: Molecular Biotechnology
Study programme type: university
Study level: graduate
Academic / professional title: magistar/magistra inženjer/inženjerka molekularne biotehnologije (magistar/magistra inženjer/inženjerka molekularne biotehnologije)
Type of resourceText
File originBorn digital
Access conditionsOpen access
Terms of useLicence In copyright icon
Created on2019-12-06 13:41:06