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master's thesis
Uzgoj ljubičastih nesumpornih bakterija u bioreaktoru s miješalom bez izvora svjetlosti na različitim izvorima ugljika
Zagreb: University of Zagreb, Faculty of Food Technology and Biotechnology, 2019. urn:nbn:hr:159:361338
Madaras, Manda
University of Zagreb
Faculty of Food Technology and Biotechnology
Department of Biochemical Engineering
Laboratory for Biochemical Engineering, Industrial Microbiology and Malting and Brewing Technology

Institutional repository: Repository of the Faculty of Food Technology and Biotechnology

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Madaras, M. (2019). Uzgoj ljubičastih nesumpornih bakterija u bioreaktoru s miješalom bez izvora svjetlosti na različitim izvorima ugljika (Master's thesis). Zagreb: University of Zagreb, Faculty of Food Technology and Biotechnology. Retrieved from https://urn.nsk.hr/urn:nbn:hr:159:361338

Madaras, Manda. "Uzgoj ljubičastih nesumpornih bakterija u bioreaktoru s miješalom bez izvora svjetlosti na različitim izvorima ugljika." Master's thesis, University of Zagreb, Faculty of Food Technology and Biotechnology, 2019. https://urn.nsk.hr/urn:nbn:hr:159:361338

Madaras, Manda. "Uzgoj ljubičastih nesumpornih bakterija u bioreaktoru s miješalom bez izvora svjetlosti na različitim izvorima ugljika." Master's thesis, University of Zagreb, Faculty of Food Technology and Biotechnology, 2019. https://urn.nsk.hr/urn:nbn:hr:159:361338

Madaras, M. (2019). 'Uzgoj ljubičastih nesumpornih bakterija u bioreaktoru s miješalom bez izvora svjetlosti na različitim izvorima ugljika', Master's thesis, University of Zagreb, Faculty of Food Technology and Biotechnology, accessed 17 June 2024, https://urn.nsk.hr/urn:nbn:hr:159:361338

Madaras M. Uzgoj ljubičastih nesumpornih bakterija u bioreaktoru s miješalom bez izvora svjetlosti na različitim izvorima ugljika [Master's thesis]. Zagreb: University of Zagreb, Faculty of Food Technology and Biotechnology; 2019 [cited 2024 June 17] Available at: https://urn.nsk.hr/urn:nbn:hr:159:361338

M. Madaras, "Uzgoj ljubičastih nesumpornih bakterija u bioreaktoru s miješalom bez izvora svjetlosti na različitim izvorima ugljika", Master's thesis, University of Zagreb, Faculty of Food Technology and Biotechnology, Zagreb, 2019. Available at: https://urn.nsk.hr/urn:nbn:hr:159:361338

Metadata
TitleUzgoj ljubičastih nesumpornih bakterija u bioreaktoru s miješalom bez izvora svjetlosti na različitim izvorima ugljika
Title (english)Cultivation of purple non-sulfur bacteria in a stirred tank bioreactor under dark conditions with different carbon sources
AuthorManda Madaras
MentorMario Novak (mentor)
GranterUniversity of Zagreb
Faculty of Food Technology and Biotechnology
(Department of Biochemical Engineering)
(Laboratory for Biochemical Engineering, Industrial Microbiology and Malting and Brewing Technology)
Zagreb
Defense date and country2019-10-22, Croatia
Scientific / art field,
discipline and subdiscipline		BIOTECHNICAL SCIENCES
Biotechnology
Abstract
Ljubičaste bakterije se kroz zadnjih nekoliko desetljeća prezentiraju kao modelni organizmi za istraživanje i shvaćanje fotosintetskih prokariota zahvaljujući njihovoj iznimnoj sposobnosti prilagodbe raznim uvjetima okoline. Također, ljubičaste nesumporne bakterije su naročito zanimljive s komercijalnog aspekta s obzirom na činjenicu da proizvode veliki niz proizvoda kao što su vodik, pigmenti, biokemikalije, biopolimeri i mnoge druge. U ovom radu se ispitivao aerobni kemoheterotrofni rast, potrošnja supstrata, te sinteza pigmenata bakterija Rhodobacter azotoformans JCM 9340 i Rhodovulum adriaticum DSM 2781 u bioreaktoru s miješalom bez prisutnosti svjetla uz različite izvore ugljika. Kao izvori ugljika su se koristile glukoza i ksiloza, te hidrolizat lignocelulozne biomase dobiven kiselinskom predobradom u visokotlačnom reaktoru. Uzgoj Rhodobacter azotoformans JCM 9340 je pokazao slabiji rast, te sintezu pigmenata tijekom uzgoja na glukozi i ksilozi, dok je Rhodovulum adriaticum DSM 2781 najveću produktivnost 0,154 g L-¹h-¹ procesa postigao tijekom uzgoja na predobrađenoj lignoceluloznoj biomasi. Također, tijekom tog procesa se postigao i najveći prinos biomase bakterije 3,7 g L-¹. Najviše vrijednosti koncentracije pigmenata, prikazane kao vrijednosti bakterioklorofila a, su postignute tijekom uzgoja Rhodovulum adriaticum DSM 2781 na glukozi i ksilozi 8,599 mg L-¹.
Abstract (english)
In the last couple of decades, purple non-sulfur bacteria have presented themselves as a model organism for researching and understanding of photosynthetic prokaryotes thanks to their excellent capability of adapting to different environmental conditions. These bacteria are also interesting in a commercial aspect due to their capability of producing a grand array of products such as biohydrogen, pigments, biochemicals, biopolymers etc. In this thesis, aerobic chemoheterotrophic growth, substrate consumption and pigment biosynthesis of bacteria Rhodovulum adriaticum DSM 2781 and Rhodobacter azotoformans JCM 9340 in a stirred tank bioreactor under dark conditions with different carbon sources, was studied. Glucose, xylose and lignocellulosic hydrolysates, obtained by acidic pretreatment in highpressure reactor, were used as carbon sources for cultivation. Results obtained by Rhodobacter azotoformans JCM 9340 showed poor growth and photosynthetic pigments synthesis on glucose and xylose, while results obtained by Rhodovulum adriaticum DSM 2781 showed great success. The highest bioprocess productivity of 0.154 g L-¹ h-¹ was observed during cultivation on pretreated lignocellulosic biomass. During that process, the highest yield of biomass of 3.7 g L-¹ was achieved, as well. The most successful medium for pigment biosynthesis, shown as bacteriochlorophyll a concentration, was glucose/xylose medium reaching 8.599 mg L-¹ of bacteriochlorophyll a.
Keywords
Keywords (english)
Languagecroatian
URN:NBNurn:nbn:hr:159:361338
Study programmeTitle: Bioprocess Engineering
Study programme type: university
Study level: graduate
Academic / professional title: magistar/magistra inženjer/inženjerka bioprocesnog inženjerstva (magistar/magistra inženjer/inženjerka bioprocesnog inženjerstva)
Type of resourceText
File originBorn digital
Access conditionsOpen access
Terms of useLicence Attribution-NoDerivatives 4.0 International (CC BY-ND 4.0) icon
Created on2019-12-11 13:52:07