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master's thesis
Povezanost jednostrukog nukleotidnog polimorfizma rs17817449 u ljudskom genu FTO s tjelesnom masom u žena Zagrebačke županije
Zagreb: University of Zagreb, Faculty of Food Technology and Biotechnology, 2016. urn:nbn:hr:159:899852
Huđek, Ana
University of Zagreb
Faculty of Food Technology and Biotechnology
Department of Biochemical Engineering
Laboratory for Biology and Microbial Genetics

Institutional repository: Repository of the Faculty of Food Technology and Biotechnology

Cite this document

Huđek, A. (2016). Povezanost jednostrukog nukleotidnog polimorfizma rs17817449 u ljudskom genu FTO s tjelesnom masom u žena Zagrebačke županije (Master's thesis). Zagreb: University of Zagreb, Faculty of Food Technology and Biotechnology. Retrieved from https://urn.nsk.hr/urn:nbn:hr:159:899852

Huđek, Ana. "Povezanost jednostrukog nukleotidnog polimorfizma rs17817449 u ljudskom genu FTO s tjelesnom masom u žena Zagrebačke županije." Master's thesis, University of Zagreb, Faculty of Food Technology and Biotechnology, 2016. https://urn.nsk.hr/urn:nbn:hr:159:899852

Huđek, Ana. "Povezanost jednostrukog nukleotidnog polimorfizma rs17817449 u ljudskom genu FTO s tjelesnom masom u žena Zagrebačke županije." Master's thesis, University of Zagreb, Faculty of Food Technology and Biotechnology, 2016. https://urn.nsk.hr/urn:nbn:hr:159:899852

Huđek, A. (2016). 'Povezanost jednostrukog nukleotidnog polimorfizma rs17817449 u ljudskom genu FTO s tjelesnom masom u žena Zagrebačke županije', Master's thesis, University of Zagreb, Faculty of Food Technology and Biotechnology, accessed 18 January 2025, https://urn.nsk.hr/urn:nbn:hr:159:899852

Huđek A. Povezanost jednostrukog nukleotidnog polimorfizma rs17817449 u ljudskom genu FTO s tjelesnom masom u žena Zagrebačke županije [Master's thesis]. Zagreb: University of Zagreb, Faculty of Food Technology and Biotechnology; 2016 [cited 2025 January 18] Available at: https://urn.nsk.hr/urn:nbn:hr:159:899852

A. Huđek, "Povezanost jednostrukog nukleotidnog polimorfizma rs17817449 u ljudskom genu FTO s tjelesnom masom u žena Zagrebačke županije", Master's thesis, University of Zagreb, Faculty of Food Technology and Biotechnology, Zagreb, 2016. Available at: https://urn.nsk.hr/urn:nbn:hr:159:899852

Metadata
TitlePovezanost jednostrukog nukleotidnog polimorfizma rs17817449 u ljudskom genu FTO s tjelesnom masom u žena Zagrebačke županije
Title (english)Association study of FTO gene single nucleotide polymorphism rs17817449 with body mass in women from the Zagreb region
AuthorAna Huđek
MentorVišnja Bačun-Družina (mentor)
GranterUniversity of Zagreb
Faculty of Food Technology and Biotechnology
(Department of Biochemical Engineering)
(Laboratory for Biology and Microbial Genetics)
Zagreb
Defense date and country2016-07-04, Croatia
Scientific / art field,
discipline and subdiscipline		BIOTECHNICAL SCIENCES
Biotechnology
Abstract
U današnjem industrijaliziranom svijetu, pretilost postaje jedna od glavnih prijetnji za zdravlje odraslih ljudi, adolescenata i djece jer nepovoljno utječe na kvalitetu života i očekivani životni vijek ljudi te potiče razvoj različitih bolesti. Etiologija pretilosti je vrlo složena i veliki broj kombinacija okolišnih faktora i faktora načina života može uzrokovati različite genetičke polimorfizme. Gen FTO (engl. Fat mass and obesity-associated gene) ističe se kao značajniji genetički uzročnik poligenske pretilosti i faktor utjecaja na indeks tjelesne mase (engl. Body Mass Indeks, BMI). Cilj ovog rada bio je utvrditi povezanost jednostrukog nukleotidnog polimorfizma (engl. Single Nucleotide Polymorphism, SNP) rs17817449 u genu FTO s BMI-om i razlikom u sastavu mikroorganizama usne šupljine u žena Zagrebačke županije normalnog BMI-a i BMI-a većeg od 30 kg m-2, a što je već prethodno dokazano za populaciju Europe, Koreje, Sjeverne Amerike i Indije bez istraživanja mikrobioma usta. Ovo istraživanje je prvo takve vrste provedeno samo na ženskoj populaciji u kontinentalnoj Hrvatskoj. Za genotipizaciju je korištena metoda lančane reakcije polimeraze u stvarnom vremenu i uočena je veća učestalost rizičnog alela G polimorfizma rs17817449 u skupini pretilih žena te njegova povezanost s BMI-om i povišenim krvnim tlakom. Također, uočene su razlike u mikrobiomu usne šupljine te razlike u životnim i prehrambenim navikama između ispitivanih skupina žena. Dobiveni rezultati trebali bi pridonijeti boljem razumijevanju uloge polimorfizma rs17817449 gena FTO, mikrobioma usta i načina života u razvoju pretilosti kod žena te samim time pomoći u razvoju terapijskih strategija u borbi protiv pretilosti i bolesti uzrokovanih pretilošću.
Abstract (english)
In today's industrialized world, obesity has become one of the major threats to the health of adults, adolescents and children. Obesity adversely affects the quality of life and life expectancy of people as well as the development of various diseases. The etiology of obesity is very complex, and any combination of environmental factors and lifestyle factors can cause a variety of genetic polymorphisms. FTO (Fat mass and obesity-associated) gene is the most significant genetic cause of polygenic obesity and factor than can affect the body mass index (BMI). The aim of this study was to show the connection between FTO gene rs17817449 SNP with BMI and the difference in the oral composition of microorganisms in women from the Zagreb region with normal BMI and BMI greater than 30 kg m-2, what has already been proven for the population of Europe, Korea, North America and India without oral microbiome research. This study is the first research on the female population in the continental Croatia. For genotyping was used real time PCR method. In DNA of obese women from Zagreb region was observed a higher frequency of the FTO gene rs17817449 SNP risk allele G and its association with BMI and hypertension. Furthermore, differences were detected in the oral microbiome and in the dietary habits between the two test groups. The results should contribute to a better understanding of the role FTO gene rs17817449 SNP, oral microbiome and lifestyle in the development of obesity and help in developing strategies for treating obesity.
Keywords
Keywords (english)
Languagecroatian
URN:NBNurn:nbn:hr:159:899852
Study programmeTitle: Molecular Biotechnology
Study programme type: university
Study level: graduate
Academic / professional title: magistar/magistra inženjer/inženjerka molekularne biotehnologije (magistar/magistra inženjer/inženjerka molekularne biotehnologije)
Type of resourceText
File originBorn digital
Access conditionsOpen access
Terms of useLicence In copyright icon
Created on2017-04-26 11:26:40