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master's thesis
Antioksidacijska aktivnost i ukupni fenoli ekstrakata medicinske gljive Ganoderma lucidum
Zagreb: University of Zagreb, Faculty of Food Technology and Biotechnology, 2019. urn:nbn:hr:159:746820
Radišić, Veronika
University of Zagreb
Faculty of Food Technology and Biotechnology
Department of Biochemical Engineering
Laboratory for Biochemical Engineering, Industrial Microbiology and Malting and Brewing Technology

Institutional repository: Repository of the Faculty of Food Technology and Biotechnology

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Radišić, V. (2019). Antioksidacijska aktivnost i ukupni fenoli ekstrakata medicinske gljive Ganoderma lucidum (Master's thesis). Zagreb: University of Zagreb, Faculty of Food Technology and Biotechnology. Retrieved from https://urn.nsk.hr/urn:nbn:hr:159:746820

Radišić, Veronika. "Antioksidacijska aktivnost i ukupni fenoli ekstrakata medicinske gljive Ganoderma lucidum." Master's thesis, University of Zagreb, Faculty of Food Technology and Biotechnology, 2019. https://urn.nsk.hr/urn:nbn:hr:159:746820

Radišić, Veronika. "Antioksidacijska aktivnost i ukupni fenoli ekstrakata medicinske gljive Ganoderma lucidum." Master's thesis, University of Zagreb, Faculty of Food Technology and Biotechnology, 2019. https://urn.nsk.hr/urn:nbn:hr:159:746820

Radišić, V. (2019). 'Antioksidacijska aktivnost i ukupni fenoli ekstrakata medicinske gljive Ganoderma lucidum', Master's thesis, University of Zagreb, Faculty of Food Technology and Biotechnology, accessed 19 November 2024, https://urn.nsk.hr/urn:nbn:hr:159:746820

Radišić V. Antioksidacijska aktivnost i ukupni fenoli ekstrakata medicinske gljive Ganoderma lucidum [Master's thesis]. Zagreb: University of Zagreb, Faculty of Food Technology and Biotechnology; 2019 [cited 2024 November 19] Available at: https://urn.nsk.hr/urn:nbn:hr:159:746820

V. Radišić, "Antioksidacijska aktivnost i ukupni fenoli ekstrakata medicinske gljive Ganoderma lucidum", Master's thesis, University of Zagreb, Faculty of Food Technology and Biotechnology, Zagreb, 2019. Available at: https://urn.nsk.hr/urn:nbn:hr:159:746820

Metadata
TitleAntioksidacijska aktivnost i ukupni fenoli ekstrakata medicinske gljive Ganoderma lucidum
Title (english)Antioxidant activity and total phenolics of medicinal mushroom Ganoderma lucidum extracts
AuthorVeronika Radišić
MentorSunčica Beluhan (mentor)
GranterUniversity of Zagreb
Faculty of Food Technology and Biotechnology
(Department of Biochemical Engineering)
(Laboratory for Biochemical Engineering, Industrial Microbiology and Malting and Brewing Technology)
Zagreb
Defense date and country2019-12-12, Croatia
Scientific / art field,
discipline and subdiscipline		BIOTECHNICAL SCIENCES
Biotechnology
Abstract
Gljiva Ganoderma lucidum je izvor bioaktivnih sastojaka, kao što su triterpeni, polisaharidi, steroidi, fenolni sastojci i glikoproteini. Polisaharidi, posebice -glukani, triterpeni i fenoli posjeduju antioksidacijska svojstva. U ovom radu su proučavani udjeli ukupnih fenolnih spojeva i antioksidacijska aktivnost u vodenim i metanolnim ekstraktima micelija i plodišta (bazidiokarpa) gljive G. lucidum. Za dokazivanje antioksidacijskog kapaciteta ekstrakata, korištene su tri temeljne metode određivanja: DPPH, ABTS i FRAP. Metanolni ekstrakt je pokazao maksimalnu sposobnost vezanja DPPH slobodnih radikala (EC50 = 0,29 mg/mL) i ABTS+ (EC50 = 0,58 mg/mL), dok je u vodenom ekstraktu izmjerena bolja sposobnost vezanja željeznih iona (EC50 = 5 μg/mL). Udjel ukupnih fenolnih spojeva i antioksidacijski kapacitet bio je značajno veći u metanolnim ekstraktima (p < 0,01). Uočena je pozitivna korelacija između koncentracije ukupnih fenola i antioksidacijske aktivnosti. Rezultatima ovog istraživanja je potvrđeno da i micelij i plodište gljive G. lucidum posjeduju osobita antioksidacijska svojstva, te se mogu koristiti kao izvrsni izvor supstancija s antioksidacijskom aktivnošću za terapeutsku primjenu.
Abstract (english)
Fungi of Ganoderma lucidum genus contain bioactive components such as triterpenes, polysaccharides, steroids, phenolic compounds and glycoproteins. Polysaccharides, particularly - glucans, triterpenes and phenolic compounds have antioxidant properties. In this work, the total phenolic contents and antioxidant activity of water and methanolic extracts of G. lucidum in mycelia and mature fruiting body (basidiocarp) were evaluated. Three complementary assays, DPPH, ABTS and FRAP were used to screen the antioxidant capacity of extracts. Both aqueous and methanolic extracts were analyzed for free radical scavenging activity. Methanolic extract showed maximum scavenging activity for DPPH (EC50 = 0.29 mg/mL) and ABTS+ (EC50 = 0.58 mg/mL), whereas aqueous extract had better scavenging for ferric reducing antioxidant power (EC50 = 5 μg/mL). Total phenolic content and total antioxidant capacity were significantly higher in methanolic extract (p < 0.01). A positive correlation existed between the phenolic content and antioxidant activity. Results indicated that both mycelia and mature fruiting body of G. lucidum possess remarkable antioxidant properties, which can be used as excellent sources of antioxidant compounds for therapeutic application.
Keywords
Keywords (english)
Languagecroatian
URN:NBNurn:nbn:hr:159:746820
Study programmeTitle: Bioprocess Engineering
Study programme type: university
Study level: graduate
Academic / professional title: magistar/magistra inženjer/inženjerka bioprocesnog inženjerstva (magistar/magistra inženjer/inženjerka bioprocesnog inženjerstva)
Type of resourceText
File originBorn digital
Access conditionsOpen access
Terms of useLicence Attribution-NoDerivatives 4.0 International (CC BY-ND 4.0) icon
Created on2019-12-31 09:25:03