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master's thesis
Utjecaj eutektičkih otapala na enzimski kataliziranu hidrolizu (R,S)-1-feniletil-acetata
Zagreb: University of Zagreb, Faculty of Food Technology and Biotechnology, 2019. urn:nbn:hr:159:362261
Kapitanović, Josipa
University of Zagreb
Faculty of Food Technology and Biotechnology
Department of Biochemical Engineering
Laboratory for Cell Technology, Application and Biotransformations

Institutional repository: Repository of the Faculty of Food Technology and Biotechnology

Cite this document

Kapitanović, J. (2019). Utjecaj eutektičkih otapala na enzimski kataliziranu hidrolizu (R,S)-1-feniletil-acetata (Master's thesis). Zagreb: University of Zagreb, Faculty of Food Technology and Biotechnology. Retrieved from https://urn.nsk.hr/urn:nbn:hr:159:362261

Kapitanović, Josipa. "Utjecaj eutektičkih otapala na enzimski kataliziranu hidrolizu (R,S)-1-feniletil-acetata." Master's thesis, University of Zagreb, Faculty of Food Technology and Biotechnology, 2019. https://urn.nsk.hr/urn:nbn:hr:159:362261

Kapitanović, Josipa. "Utjecaj eutektičkih otapala na enzimski kataliziranu hidrolizu (R,S)-1-feniletil-acetata." Master's thesis, University of Zagreb, Faculty of Food Technology and Biotechnology, 2019. https://urn.nsk.hr/urn:nbn:hr:159:362261

Kapitanović, J. (2019). 'Utjecaj eutektičkih otapala na enzimski kataliziranu hidrolizu (R,S)-1-feniletil-acetata', Master's thesis, University of Zagreb, Faculty of Food Technology and Biotechnology, accessed 28 June 2024, https://urn.nsk.hr/urn:nbn:hr:159:362261

Kapitanović J. Utjecaj eutektičkih otapala na enzimski kataliziranu hidrolizu (R,S)-1-feniletil-acetata [Master's thesis]. Zagreb: University of Zagreb, Faculty of Food Technology and Biotechnology; 2019 [cited 2024 June 28] Available at: https://urn.nsk.hr/urn:nbn:hr:159:362261

J. Kapitanović, "Utjecaj eutektičkih otapala na enzimski kataliziranu hidrolizu (R,S)-1-feniletil-acetata", Master's thesis, University of Zagreb, Faculty of Food Technology and Biotechnology, Zagreb, 2019. Available at: https://urn.nsk.hr/urn:nbn:hr:159:362261

Metadata
TitleUtjecaj eutektičkih otapala na enzimski kataliziranu hidrolizu (R,S)-1-feniletil-acetata
Title (english)The effect of deep eutectic solvents on lipase catalyzed hydrolysis of (R,S)-1-phenylethylcetate
AuthorJosipa Kapitanović
MentorMarina Cvjetko Bubalo (mentor)
GranterUniversity of Zagreb
Faculty of Food Technology and Biotechnology
(Department of Biochemical Engineering)
(Laboratory for Cell Technology, Application and Biotransformations)
Zagreb
Defense date and country2019-09-20, Croatia
Scientific / art field,
discipline and subdiscipline		BIOTECHNICAL SCIENCES
Biotechnology
Abstract
Posljednjih nekoliko godina raste broj istraživanja usmjerenih na mogućnost primjene eutektičkih otapala u različitim biotransformacijskim procesima. Glavne prednosti njihove primjene u odnosu na ostala otapala su biorazgradivost, netoksičnost, nehlapljivost te jednostavna priprava. U ovom radu ispitana je mogućnost primjene imobilizirane lipaze te hidrolitičkih enzima iz narančine kore kao biokatalizatora u hidrolizi (R,S)-1-feniletil-acetata u prirodnom eutektičkom otapalu kolin-klorid:etilen-glikol (ChClEG) s različitim udjelima vode. Uspoređujući postotak iskorištenja hidrolize i enantiomernog viška, utvrđeno je da je najpogodnije otapalo ChClEG s 50 % udjela vode (w/w). Također, ispitana je skladišna stabilnost imobilizirane lipaze B te hidrolitičkih enzima narančine kore kroz vremenski period od 21 dan u eutektičkom otapalu u ChClEG. Iz dobivenih vrijednosti rezidualne aktivnosti utvrđeno je da su hidrolitički enzimi iz narančine kore stabilniji u ChClEG nego u puferu, dok je lipaza stabilnija u puferu nego u ChClEG s 50 % udjela vode (w/w).
Abstract (english)
In the last few years, there has been an increasing number of studies focused on the possibilities of using eutectic solvents in various biotransformation processes. The main advantages of their application over other solvents are biodegradability, non-toxicity, non-volatility and easy synthesis. This work examines the possibility of using immobilized lipase and hydrolytic enzymes from orange peel as biocatalysts in the hydrolysis of (R,S)-1-phenylethyl acetate in a natural deep eutectic solvent choline chloride:ethylene glycol with different water contents. Comparing the percentage of utilization of hydrolysis and enantiomeric excess, it was found that the most suitable solvent is ChClEG with 50% water content (w/w). Also, the storage thermal stability of immobilized lipase and hydrolytic enzymes from orange peel over a period of 21 days was examined. From the obtained residual activity values, it was found that the hydrolytic enzymes from the orange peel were more stable in ChClEG than in buffer, while lipase was more stable in buffer than in ChClEG with 50% water content (w/w).
Keywords
Keywords (english)
Languagecroatian
URN:NBNurn:nbn:hr:159:362261
Study programmeTitle: Molecular Biotechnology
Study programme type: university
Study level: graduate
Academic / professional title: magistar/magistra inženjer/inženjerka molekularne biotehnologije (magistar/magistra inženjer/inženjerka molekularne biotehnologije)
Type of resourceText
File originBorn digital
Access conditionsOpen access
Terms of useLicence Attribution-NoDerivatives 4.0 International (CC BY-ND 4.0) icon
Created on2020-01-28 13:16:29