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master's thesis
Simultana saharifikacija i fermentacija predtretiranih kukuruznih oklasaka pomoću plijesni Mucor indicus
Zagreb: University of Zagreb, Faculty of Food Technology and Biotechnology, 2019. urn:nbn:hr:159:271684
Jedrejčić, Petra
University of Zagreb
Faculty of Food Technology and Biotechnology
Department of Biochemical Engineering
Laboratory for Biochemical Engineering, Industrial Microbiology and Malting and Brewing Technology

Institutional repository: Repository of the Faculty of Food Technology and Biotechnology

Cite this document

Jedrejčić, P. (2019). Simultana saharifikacija i fermentacija predtretiranih kukuruznih oklasaka pomoću plijesni Mucor indicus (Master's thesis). Zagreb: University of Zagreb, Faculty of Food Technology and Biotechnology. Retrieved from https://urn.nsk.hr/urn:nbn:hr:159:271684

Jedrejčić, Petra. "Simultana saharifikacija i fermentacija predtretiranih kukuruznih oklasaka pomoću plijesni Mucor indicus." Master's thesis, University of Zagreb, Faculty of Food Technology and Biotechnology, 2019. https://urn.nsk.hr/urn:nbn:hr:159:271684

Jedrejčić, Petra. "Simultana saharifikacija i fermentacija predtretiranih kukuruznih oklasaka pomoću plijesni Mucor indicus." Master's thesis, University of Zagreb, Faculty of Food Technology and Biotechnology, 2019. https://urn.nsk.hr/urn:nbn:hr:159:271684

Jedrejčić, P. (2019). 'Simultana saharifikacija i fermentacija predtretiranih kukuruznih oklasaka pomoću plijesni Mucor indicus', Master's thesis, University of Zagreb, Faculty of Food Technology and Biotechnology, accessed 02 January 2025, https://urn.nsk.hr/urn:nbn:hr:159:271684

Jedrejčić P. Simultana saharifikacija i fermentacija predtretiranih kukuruznih oklasaka pomoću plijesni Mucor indicus [Master's thesis]. Zagreb: University of Zagreb, Faculty of Food Technology and Biotechnology; 2019 [cited 2025 January 02] Available at: https://urn.nsk.hr/urn:nbn:hr:159:271684

P. Jedrejčić, "Simultana saharifikacija i fermentacija predtretiranih kukuruznih oklasaka pomoću plijesni Mucor indicus", Master's thesis, University of Zagreb, Faculty of Food Technology and Biotechnology, Zagreb, 2019. Available at: https://urn.nsk.hr/urn:nbn:hr:159:271684

Metadata
TitleSimultana saharifikacija i fermentacija predtretiranih kukuruznih oklasaka pomoću plijesni Mucor indicus
Title (english)Simultaneous saccharification and fermentation of pretreated corn cobs by fungus Mucor indicus
AuthorPetra Jedrejčić
MentorMario Novak (mentor)
GranterUniversity of Zagreb
Faculty of Food Technology and Biotechnology
(Department of Biochemical Engineering)
(Laboratory for Biochemical Engineering, Industrial Microbiology and Malting and Brewing Technology)
Zagreb
Defense date and country2019-09-19, Croatia
Scientific / art field,
discipline and subdiscipline		BIOTECHNICAL SCIENCES
Biotechnology
Abstract
U ovom radu proveden je proces simultane enzimske saharifikacije i fermentacije ispranog i usitnjenog čvrstog dijela zaostalog nakon kiselinske predobrade kukuruznih oklasaka. U cilju uspješne provedbe procesa istražen je utjecaj dva različita enzimska pripravka (Cellulase Blend-a i Viscozyme-a) u koncentraciji od 10 % (vol/vol) te njihove kombinacije, 2 % (vol/vol) Cellulase Blend i 5 % (vol/vol) Viscozyme na iskorištenje hidrolize glukana, ksilana i ukupnih ugljikohidrata korištene sirovine. Najviši stupanj hidrolize glukana (100 %) i ukupnih ugljikohidrata (89,90 %) dobiven je uz 10 % (vol/vol) Cellulase Blend na 40 °C u hranjivoj podlozi, a ksilana (93,51 %) uz 10 % (vol/vol) Viscozyme pri 40 °C u puferu. Kako bi se ispitao utjecaj temperature i podloge na uspješnost procesa proizvodnje bioetanola pomoću plijesni M. indicus proveden je aerobni uzgoj u Erlenmayer tikvicama tehnikom simultane enzimske saharifikacije i fermentacije na 30 i 40 °C u puferu i podlozi. Najveća uspješnost procesa proizvodnje etanola zabilježena je kod uzgoja u podlozi uz dodatak 4 % (vol/vol) Cellulase Blend-a pri 30 °C, a produktivnost je iznosila 0,0939 g L-1 h-1. U istim uvjetima te uz dodatak istog enzimskog pripravka koncentracije 4 % (vol/vol) proveden je uzgoj M. indicus u bioreaktoru s miješalom, a produktivnost je nakon 22 h uzgoja iznosila 0,1432 g L-1 h-1. Nakon uzgoja provedena je izolacija glukozamina.
Abstract (english)
In this paper, the procedure of simultaneous saccharification and fermentation of washed and shredded solid residues after dilute acid pretreatment of corn cobs was performed. In order to successfully carry out the process, the effect of two different enzyme blends (Cellulase Blend and Viscozyme at a concentrations of 10 % (vol/vol)) and their combination, 2 % (vol/vol) Cellulase Blend and 5 % (vol/vol) Viscozyme) on the efficiency of hydrolysis of glucan, xylan and total carbohydrates of the raw material used was investigated. The highest degree of hydrolysis of glucan (100 %) and total carbohydrates (89.90 %) was obtained with a 10 % (vol/vol) Cellulase Blend at 40 °C in the matrix which simulates the broth, and xylan (93.51 %) with 10 % (vol/vol) Viscozyme at 40 °C in acetate buffer. In order to examine the influence of temperature and media on the success of the bioethanol production process by M. indicus, aerobic cultivation was performed in Erlenmayer flasks by the technique of simultaneous enzymatic saccharification and fermentation at 30 and 40 °C in two different broths. The highest bioprocess productivity of 0.0939 g L-1 h-1 was observed in broth with the addition of 4 % (vol/vol) Cellulase Blend at 30 °C. M. indicus was grown in a stirred tank bioreactor under the same conditions and with the addition of the same enzyme blend at a concentration of 4 % (vol/vol). After 22 hours of cultivation the productivity reached value of 0.1432 g L-1 h-1. The isolation of glucosamine was performed after cultivation.
Keywords
Keywords (english)
Languagecroatian
URN:NBNurn:nbn:hr:159:271684
Study programmeTitle: Bioprocess Engineering
Study programme type: university
Study level: graduate
Academic / professional title: magistar/magistra inženjer/inženjerka bioprocesnog inženjerstva (magistar/magistra inženjer/inženjerka bioprocesnog inženjerstva)
Type of resourceText
File originBorn digital
Access conditionsOpen access
Terms of useLicence Attribution-NoDerivatives 4.0 International (CC BY-ND 4.0) icon
Created on2020-01-28 14:19:40