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master's thesis
In vitro istraživanje adjuvantnog učinka quisinostata na citotoksičnost 5-fluorouracila na tumorske stanice mliječne žlijezde miša
Zagreb: University of Zagreb, Faculty of Food Technology and Biotechnology, 2019. urn:nbn:hr:159:032637
Moslavac, Leonina
University of Zagreb
Faculty of Food Technology and Biotechnology
Department of Biochemical Engineering
Laboratory for Biology and Microbial Genetics

Institutional repository: Repository of the Faculty of Food Technology and Biotechnology

Cite this document

Moslavac, L. (2019). In vitro istraživanje adjuvantnog učinka quisinostata na citotoksičnost 5-fluorouracila na tumorske stanice mliječne žlijezde miša (Master's thesis). Zagreb: University of Zagreb, Faculty of Food Technology and Biotechnology. Retrieved from https://urn.nsk.hr/urn:nbn:hr:159:032637

Moslavac, Leonina. "In vitro istraživanje adjuvantnog učinka quisinostata na citotoksičnost 5-fluorouracila na tumorske stanice mliječne žlijezde miša." Master's thesis, University of Zagreb, Faculty of Food Technology and Biotechnology, 2019. https://urn.nsk.hr/urn:nbn:hr:159:032637

Moslavac, Leonina. "In vitro istraživanje adjuvantnog učinka quisinostata na citotoksičnost 5-fluorouracila na tumorske stanice mliječne žlijezde miša." Master's thesis, University of Zagreb, Faculty of Food Technology and Biotechnology, 2019. https://urn.nsk.hr/urn:nbn:hr:159:032637

Moslavac, L. (2019). 'In vitro istraživanje adjuvantnog učinka quisinostata na citotoksičnost 5-fluorouracila na tumorske stanice mliječne žlijezde miša', Master's thesis, University of Zagreb, Faculty of Food Technology and Biotechnology, accessed 18 February 2025, https://urn.nsk.hr/urn:nbn:hr:159:032637

Moslavac L. In vitro istraživanje adjuvantnog učinka quisinostata na citotoksičnost 5-fluorouracila na tumorske stanice mliječne žlijezde miša [Master's thesis]. Zagreb: University of Zagreb, Faculty of Food Technology and Biotechnology; 2019 [cited 2025 February 18] Available at: https://urn.nsk.hr/urn:nbn:hr:159:032637

L. Moslavac, "In vitro istraživanje adjuvantnog učinka quisinostata na citotoksičnost 5-fluorouracila na tumorske stanice mliječne žlijezde miša", Master's thesis, University of Zagreb, Faculty of Food Technology and Biotechnology, Zagreb, 2019. Available at: https://urn.nsk.hr/urn:nbn:hr:159:032637

Metadata
TitleIn vitro istraživanje adjuvantnog učinka quisinostata na citotoksičnost 5-fluorouracila na tumorske stanice mliječne žlijezde miša
Title (english)In vitro study of the adjuvant effect of quisinostat on the cytotoxicity of 5-fluorouracile on mouse mammary gland tumor cells
AuthorLeonina Moslavac
MentorLidija Šver (mentor)
GranterUniversity of Zagreb
Faculty of Food Technology and Biotechnology
(Department of Biochemical Engineering)
(Laboratory for Biology and Microbial Genetics)
Zagreb
Defense date and country2019-09-25, Croatia
Scientific / art field,
discipline and subdiscipline		BIOTECHNICAL SCIENCES
Biotechnology
Abstract
Tumori su osnovni predmet istraživanja velike većine eksperimentalnih radova zbog pojave rastuće smrtnosti, neovisno o dobi i spolu. Tumor dojke najzastupljeniji je oblik kod ženske populacije te je kao takav predmet istraživanja ovog rada, s ciljem ispitivanja osjetljivosti stanica tumora mliječne žlijezde miša na primijenjeni lijek 5-fluorouracil (5-FU) te pomoćno sredstvo quisinostat (inhibitor histon deacetilaza), u obliku 2D i 3D staničnih kultura. Pritom, odreĎena je koncentracija lijeka koja daje približno 50% preživljenja stanica te efikasnost istovremenog tretmana s 5-FU i qusinostatom te predtretmana qusinostatom uz dodatak lijeka. Ispitan je utjecaj različitih koncentracija lijeka 5-FU te koncentracije qusinostata. Za odreĎivanje 2D staničnih kultura korišten je resazurin test, dok su za 3D stanične kulture korišteni CellTox™ Green Cytotoxicity, RealTime-Glo MT Cell Viability i CellTiter-Glo 3D Cell Viability test. Zaključno, 3D stanična kultura pokazala je veću osjetljivost na istovremeni tretman u odnosu na 2D staničnu kulturu, koji se pokazao kao najbolja opcija primjene u tretiranju stanica tumora mliječne žlijezde miša.
Abstract (english)
Tumors are the main subject of research in the vast majority of experimental studies due to the onset of increasing mortality, regardless of age and gender. Breast cancer is the most prevalent form in the female population, and as such is the subject of research in this study, with the aim of examining the sensitivity of mouse mammary gland tumor cells to the drug 5-fluorouracile (5-FU) and the adjuvant quisinostat (histone deacetylase inhibitor), in the form of 2D and 3D cell cultures. The concentration of the drug giving approximately 50% cell survival and the efficacy of combination treatment with 5-FU and quisinostat and pretreatment with qusinostat with drug addition were determined. The effect of different concentrations of 5-FU and the concentrations of qusinostat were examined. Resazurin assay was used to determine viability in 2D cell cultures, whereas CellTox ™ Green Cytotoxicity, RealTime-Glo MT Cell Viability and CellTiter-Glo 3D Cell Viability Assay were used for 3D cell cultures. To conclude, 3D cell culture showed greater sensitivity to combination treatment than 2D cell culture, which has proven to be the best application option in the treatment of mouse mammary gland tumor cells.
Keywords
Keywords (english)
Languagecroatian
URN:NBNurn:nbn:hr:159:032637
Study programmeTitle: Molecular Biotechnology
Study programme type: university
Study level: graduate
Academic / professional title: magistar/magistra inženjer/inženjerka molekularne biotehnologije (magistar/magistra inženjer/inženjerka molekularne biotehnologije)
Type of resourceText
File originBorn digital
Access conditionsOpen access
Terms of useLicence Attribution-NoDerivatives 4.0 International (CC BY-ND 4.0) icon
Created on2020-02-13 11:59:07