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master's thesis
Optimizacija uvjeta fototrofnog uzgoja i karakterizacija dijatomeje izolirane iz Jadranskog mora
Zagreb: University of Zagreb, Faculty of Food Technology and Biotechnology, 2019. urn:nbn:hr:159:879237
Brajković, Antonija
University of Zagreb
Faculty of Food Technology and Biotechnology
Department of Biochemical Engineering
Laboratory for Biochemical Engineering, Industrial Microbiology and Malting and Brewing Technology

Institutional repository: Repository of the Faculty of Food Technology and Biotechnology

Cite this document

Brajković, A. (2019). Optimizacija uvjeta fototrofnog uzgoja i karakterizacija dijatomeje izolirane iz Jadranskog mora (Master's thesis). Zagreb: University of Zagreb, Faculty of Food Technology and Biotechnology. Retrieved from https://urn.nsk.hr/urn:nbn:hr:159:879237

Brajković, Antonija. "Optimizacija uvjeta fototrofnog uzgoja i karakterizacija dijatomeje izolirane iz Jadranskog mora." Master's thesis, University of Zagreb, Faculty of Food Technology and Biotechnology, 2019. https://urn.nsk.hr/urn:nbn:hr:159:879237

Brajković, Antonija. "Optimizacija uvjeta fototrofnog uzgoja i karakterizacija dijatomeje izolirane iz Jadranskog mora." Master's thesis, University of Zagreb, Faculty of Food Technology and Biotechnology, 2019. https://urn.nsk.hr/urn:nbn:hr:159:879237

Brajković, A. (2019). 'Optimizacija uvjeta fototrofnog uzgoja i karakterizacija dijatomeje izolirane iz Jadranskog mora', Master's thesis, University of Zagreb, Faculty of Food Technology and Biotechnology, accessed 19 December 2024, https://urn.nsk.hr/urn:nbn:hr:159:879237

Brajković A. Optimizacija uvjeta fototrofnog uzgoja i karakterizacija dijatomeje izolirane iz Jadranskog mora [Master's thesis]. Zagreb: University of Zagreb, Faculty of Food Technology and Biotechnology; 2019 [cited 2024 December 19] Available at: https://urn.nsk.hr/urn:nbn:hr:159:879237

A. Brajković, "Optimizacija uvjeta fototrofnog uzgoja i karakterizacija dijatomeje izolirane iz Jadranskog mora", Master's thesis, University of Zagreb, Faculty of Food Technology and Biotechnology, Zagreb, 2019. Available at: https://urn.nsk.hr/urn:nbn:hr:159:879237

Metadata
TitleOptimizacija uvjeta fototrofnog uzgoja i karakterizacija dijatomeje izolirane iz Jadranskog mora
Title (english)Optimization of phototrophic cultivation conditions and characterization of diatom isolated from the Adriatic sea
AuthorAntonija Brajković
MentorMirela Ivančić - Šantek (mentor)
GranterUniversity of Zagreb
Faculty of Food Technology and Biotechnology
(Department of Biochemical Engineering)
(Laboratory for Biochemical Engineering, Industrial Microbiology and Malting and Brewing Technology)
Zagreb
Defense date and country2019-09-20, Croatia
Scientific / art field,
discipline and subdiscipline		BIOTECHNICAL SCIENCES
Biotechnology
Abstract
Dijatomeje su prepoznate kao izvrsna sirovina za proizvodnju obnovljivih biogoriva, prehrambenih, farmaceutskih i nutraceutskih proizvoda. U ovom istraživanju optimiran je sastav podloge za fototrofni uzgoj dijatomeje izolirane iz Jadranskog mora. Najveća specifična brzina rasta od 0,371 dan -1 i koncentracije biomase od 0,47 g L-1 postignuta je na deset puta koncentriranoj f/2 podlozi. Proveden je polukontinuirani uzgoj dijatomeje kako bi se povećala gustoća fototrofne kulture. Maksimalna produktivnost biomase od 0,061 g L-1 d-1 i produktivnost biosinteze lipida od 14,64 mg L-1 d-1 postignuta je treći dan nakon prve prihrane. Najveća produktivnost biosinteze eikosapentaenoične kiseline od 3,28 mg L-1 dan-1 postignuta je u četvrtom danu uzgoja. Biomasa je stanica sadržavala klorofile i karotenoide, od kojih je najzastupljeniji bio fukoksantin. Metanolski i heksanski ekstrakti dijatomeje pokazali su snažan antibakterijski učinak na rast Staphylococcus aureus i Pseudomonas aeruroginosa.
Abstract (english)
Diatoms are being recognised as an excellent source for production of renewable biofuels, food, pharmaceutical and nutraceutical products. In the present research, composition of the growth media for phototrophic growth of diatom isolated from Adriatic Sea was optimized. The highest specific rate and biomass concentration of 0,371 day -1 and 0,47 g L-1, respectively, was achieved in ten times concentrated f/2 media. In order to increase cell density of phototrophic culture, repeated batch cultivation of diatom was conduct. The maximum biomass and lipid productivity of 0,061 g L-1 d-1 and 14,64 mg L-1 d-1, respectively, was reached third day after the first culture feed. The highest productivity of eicosapentaenoic acid of 3,28 mg L-1 dan-1 was obtained fourth day of cultivation. Cell biomass contained chlorophylls and carotenoids, with fucoxanthin as the most abundant pigment. Methanol and hexane extracts of diatoms showed strong antibacterial activity against Staphylococcus aureus and Pseudomonas aeruginosa.
Keywords
Keywords (english)
Languagecroatian
URN:NBNurn:nbn:hr:159:879237
Study programmeTitle: Bioprocess Engineering
Study programme type: university
Study level: graduate
Academic / professional title: magistar/magistra inženjer/inženjerka bioprocesnog inženjerstva (magistar/magistra inženjer/inženjerka bioprocesnog inženjerstva)
Type of resourceText
File originBorn digital
Access conditionsOpen access
Terms of useLicence Attribution-NoDerivatives 4.0 International (CC BY-ND 4.0) icon
Created on2020-02-13 14:34:43