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master's thesis
Istraživanje biokompatibilnosti izvanstaničnog matriksa jetre i elektroispredanih nosača od poli-ε-kaprolaktona
Zagreb: University of Zagreb, Faculty of Food Technology and Biotechnology, 2019. urn:nbn:hr:159:672761
Ivančić, Fran
University of Zagreb
Faculty of Food Technology and Biotechnology
Department of Biochemical Engineering
Laboratory for Cell Technology, Application and Biotransformations

Institutional repository: Repository of the Faculty of Food Technology and Biotechnology

Cite this document

Ivančić, F. (2019). Istraživanje biokompatibilnosti izvanstaničnog matriksa jetre i elektroispredanih nosača od poli-ε-kaprolaktona (Master's thesis). Zagreb: University of Zagreb, Faculty of Food Technology and Biotechnology. Retrieved from https://urn.nsk.hr/urn:nbn:hr:159:672761

Ivančić, Fran. "Istraživanje biokompatibilnosti izvanstaničnog matriksa jetre i elektroispredanih nosača od poli-ε-kaprolaktona." Master's thesis, University of Zagreb, Faculty of Food Technology and Biotechnology, 2019. https://urn.nsk.hr/urn:nbn:hr:159:672761

Ivančić, Fran. "Istraživanje biokompatibilnosti izvanstaničnog matriksa jetre i elektroispredanih nosača od poli-ε-kaprolaktona." Master's thesis, University of Zagreb, Faculty of Food Technology and Biotechnology, 2019. https://urn.nsk.hr/urn:nbn:hr:159:672761

Ivančić, F. (2019). 'Istraživanje biokompatibilnosti izvanstaničnog matriksa jetre i elektroispredanih nosača od poli-ε-kaprolaktona', Master's thesis, University of Zagreb, Faculty of Food Technology and Biotechnology, accessed 06 May 2024, https://urn.nsk.hr/urn:nbn:hr:159:672761

Ivančić F. Istraživanje biokompatibilnosti izvanstaničnog matriksa jetre i elektroispredanih nosača od poli-ε-kaprolaktona [Master's thesis]. Zagreb: University of Zagreb, Faculty of Food Technology and Biotechnology; 2019 [cited 2024 May 06] Available at: https://urn.nsk.hr/urn:nbn:hr:159:672761

F. Ivančić, "Istraživanje biokompatibilnosti izvanstaničnog matriksa jetre i elektroispredanih nosača od poli-ε-kaprolaktona", Master's thesis, University of Zagreb, Faculty of Food Technology and Biotechnology, Zagreb, 2019. Available at: https://urn.nsk.hr/urn:nbn:hr:159:672761

Metadata
TitleIstraživanje biokompatibilnosti izvanstaničnog matriksa jetre i elektroispredanih nosača od poli-ε-kaprolaktona
Title (english)Biocompatibility study of extracellular liver matrix and electrospun poli-ε-caprolactone scaffolds
AuthorFran Ivančić
MentorIgor Slivac (mentor)
GranterUniversity of Zagreb
Faculty of Food Technology and Biotechnology
(Department of Biochemical Engineering)
(Laboratory for Cell Technology, Application and Biotransformations)
Zagreb
Defense date and country2019-09-23, Croatia
Scientific / art field,
discipline and subdiscipline		BIOTECHNICAL SCIENCES
Biotechnology
Abstract
Razvojem tehnika tkivnog inženjerstva omogućila se izrada nosača poželjnih mehaničkih i bioaktivnih svojstava potrebnih za regeneraciju tkiva. Dok alternativu predstavljaju tehnike transplantacijske medicine, metode tkivnog inženjerstva prednjače u in vitro modelima za istraživanje raka te razvoja farmaceutika. Cilj ovog rada bio je ispitati i usporediti biokompatibilnost elektroispredanog sintetskog i kompozitnog nosača sa prirodnim nosačem, nacijepljenih s HeLa stanicama. Nosači su bili izrađeni od polikaprolaktona (PCL), PCL-a kombiniranog sa svilinim fibroinom (PCL-SF) te izvanstaničnog matriksa svinjske jetre (ECM). U svrhu istraživanja, testovi biokompatibilnosti nosača sastojali su se od analize uspješnosti nacjepljivanja stanica te dinamike rasta i transfekcije. Kroz sva tri testa, najbolje rezultate je dao nosač od PCL-SF, dok su najmanje uspješni bili oni na ECM nosaču. Rezultati s primjenom kulture HeLa stanica u izradi modelnog tkiva pokazuju da je tehnologija nano-vlakana napredna tehnika u stvaranju staničnih nosača koji uspješno oponašaju potporanj prirodnih tkiva.
Abstract (english)
The development of tissue engineering enabled the production of cell scaffolds with superior mechanical and bioactive characteristics needed for tissue regeneration. While regenerative medicine offers an acceptable alternative, current methods in tissue engineering still lead the way in in vitro cancer model research as well as drug development. The aim of this study was to test and compare biocompatibility of HeLa seeded, electrospun polymeric synthetic and composite scaffolds with a natural scaffold. The scaffolds were made of polycaprolacton (PCL), PCL composite integrated with silk fibroin protein (PCL-SF) and extracellular matrix isolated from pork liver (ECM). In studying the biocompatibility of scaffolds, three tests were performed: cell seeding efficiency, growth dynamics and transfection efficiency. PCL-SF proved the most successful in all three tests, while porcine ECM performed the worst. HeLa culture results indicate the nano-fibre technology as an advanced technique that is indeed able to successfully mimic the 3D architecture of native tissues.
Keywords
Keywords (english)
Languagecroatian
URN:NBNurn:nbn:hr:159:672761
Study programmeTitle: Bioprocess Engineering
Study programme type: university
Study level: graduate
Academic / professional title: magistar/magistra inženjer/inženjerka bioprocesnog inženjerstva (magistar/magistra inženjer/inženjerka bioprocesnog inženjerstva)
Type of resourceText
File originBorn digital
Access conditionsOpen access
Terms of useLicence Attribution-NoDerivatives 4.0 International (CC BY-ND 4.0) icon
Created on2020-02-13 14:58:57