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master's thesis
Razvoj lipazom katalizirane sinteze (R)-feniletanola u prirodnom eutektičkom otapalu
Zagreb: University of Zagreb, Faculty of Food Technology and Biotechnology, 2020. urn:nbn:hr:159:475934
Maros, Izabela
University of Zagreb
Faculty of Food Technology and Biotechnology
Department of Biochemical Engineering
Laboratory for Cell Technology, Application and Biotransformations

Institutional repository: Repository of the Faculty of Food Technology and Biotechnology

Cite this document

Maros, I. (2020). Razvoj lipazom katalizirane sinteze (R)-feniletanola u prirodnom eutektičkom otapalu (Master's thesis). Zagreb: University of Zagreb, Faculty of Food Technology and Biotechnology. Retrieved from https://urn.nsk.hr/urn:nbn:hr:159:475934

Maros, Izabela. "Razvoj lipazom katalizirane sinteze (R)-feniletanola u prirodnom eutektičkom otapalu." Master's thesis, University of Zagreb, Faculty of Food Technology and Biotechnology, 2020. https://urn.nsk.hr/urn:nbn:hr:159:475934

Maros, Izabela. "Razvoj lipazom katalizirane sinteze (R)-feniletanola u prirodnom eutektičkom otapalu." Master's thesis, University of Zagreb, Faculty of Food Technology and Biotechnology, 2020. https://urn.nsk.hr/urn:nbn:hr:159:475934

Maros, I. (2020). 'Razvoj lipazom katalizirane sinteze (R)-feniletanola u prirodnom eutektičkom otapalu', Master's thesis, University of Zagreb, Faculty of Food Technology and Biotechnology, accessed 18 February 2025, https://urn.nsk.hr/urn:nbn:hr:159:475934

Maros I. Razvoj lipazom katalizirane sinteze (R)-feniletanola u prirodnom eutektičkom otapalu [Master's thesis]. Zagreb: University of Zagreb, Faculty of Food Technology and Biotechnology; 2020 [cited 2025 February 18] Available at: https://urn.nsk.hr/urn:nbn:hr:159:475934

I. Maros, "Razvoj lipazom katalizirane sinteze (R)-feniletanola u prirodnom eutektičkom otapalu", Master's thesis, University of Zagreb, Faculty of Food Technology and Biotechnology, Zagreb, 2020. Available at: https://urn.nsk.hr/urn:nbn:hr:159:475934

Metadata
TitleRazvoj lipazom katalizirane sinteze (R)-feniletanola u prirodnom eutektičkom otapalu
Title (english)Development of lipase-catalyzed synthesis of (R)-phenylethanol in a natural deep eutectic solvent
AuthorIzabela Maros
MentorMarina Cvjetko Bubalo (mentor)
GranterUniversity of Zagreb
Faculty of Food Technology and Biotechnology
(Department of Biochemical Engineering)
(Laboratory for Cell Technology, Application and Biotransformations)
Zagreb
Defense date and country2020-06-09, Croatia
Scientific / art field,
discipline and subdiscipline		BIOTECHNICAL SCIENCES
Biotechnology
Abstract
Mnogi industrijski procesi koriste velike količine hlapivih, zapaljivih i toksičnih organskih otapala što rezultira onečišćenjem prirode i narušavanjem ekološke ravnoteže. Prirodna eutektička otapala pripadaju novoj generaciji nehalapljivih i stabilnih otapala te su se pokazala su se kao dobra zamjena za tradicionalna organska otapala u kemijskoj i biotehnološkoj industriji. U ovom radu ispitana je mogućnost primjene nekoliko prirodnih eutektičkih otapala u enantioselektivnoj hidrolizi (R, S)-1-feniletil-acetata uz lipazu B kao biokatalizator za pripravu komercijalno zanimljivog kiralnog alkohola (R)-feniletanola. Prirodna eutektička otapala pokazala su se povoljnim otapalima za ispitivanu hidrolizu obzirom na ispitane parametre, pri čemu se otapalo kolin-klorid:etilen glikol pokazalo najpovoljnijim. Optimiranjem reakcijskih uvjeta pronađeni su optimalni uvjeti za hidrolizu, a koji se odnose na omjer enzima i supstrata, temperaturu i udio vode. Osim toga provedena je reciklacija enzima i otapala čime se pokazala mogućnost njihove ponovne upotrebe. Na temelju dobivenih rezultata može se zaključiti kako je primjena biokatalize i prirodnih eutektičkih otapala vrlo učinkovita, ekološki i ekonomski prihvatljiva metoda za proizvodnju (R)-1-feniletanola.
Abstract (english)
Many industrial processes use large quantities of volatile, flammable and toxic organic solvents resulting in pollution of nature and disturbance of ecological balance. Natural deep eutectic solvents belong to the new generation of non-volatile and stabile solvents, and are intensively studied as a replacement for organic solvents in chemical and biotechnological industry. This study examines the possibility of using several natural deep eutectic solvents for enantioselective hydrolysis of (R, S)-1-phenylethyl acetate with lipase B as biocatalyst for the preparation of a commercially interesting chiral alcohol (R)-phenyethanol. All enantioselective reactions in natural deep eutectic solvents were successfully conducted according to all tested parameters, where solvent choline chloride:ethylene glycol was found to be the best choice for reaction tested. The reaction conditions were optimized according to ratio of enzyme and substrate, temperature and water content. In addition, the recycling of enzymes and solvents was carried out, demonstrating the possibility of their reuse. Based on the results obtained, it can be concluded that the application of biocatalysis and natural deep eutectic solvents could be very efficient, environmentally and economically acceptable method for the production of (R)-1-phenylethanol.
Keywords
Keywords (english)
Languagecroatian
URN:NBNurn:nbn:hr:159:475934
Study programmeTitle: Biotechnology; specializations in: Biochemical Engineering
Course: Biochemical Engineering
Study programme type: university
Study level: graduate
Academic / professional title: diplomirani inženjer biotehnologije; smjer biokemijsko inženjerstvo (diplomirani inženjer biotehnologije; smjer biokemijsko inženjerstvo)
Type of resourceText
File originBorn digital
Access conditionsOpen access
Terms of useLicence Attribution-NoDerivatives 4.0 International (CC BY-ND 4.0) icon
Created on2020-06-19 10:29:52