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master's thesis
Optimizacija uzgoja virusa sličnog varijantu 2 u svrhu razvoja cjepiva protiv ptičjeg infektivnog bronhitisa
Zagreb: University of Zagreb, Faculty of Food Technology and Biotechnology, 2020. urn:nbn:hr:159:934593
Ćurčibašić, Valentina
University of Zagreb
Faculty of Food Technology and Biotechnology
Department of Biochemical Engineering
Laboratory for Antibiotic, Enzyme, Probiotic and Starter Cultures Technology

Institutional repository: Repository of the Faculty of Food Technology and Biotechnology

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Ćurčibašić, V. (2020). Optimizacija uzgoja virusa sličnog varijantu 2 u svrhu razvoja cjepiva protiv ptičjeg infektivnog bronhitisa (Master's thesis). Zagreb: University of Zagreb, Faculty of Food Technology and Biotechnology. Retrieved from https://urn.nsk.hr/urn:nbn:hr:159:934593

Ćurčibašić, Valentina. "Optimizacija uzgoja virusa sličnog varijantu 2 u svrhu razvoja cjepiva protiv ptičjeg infektivnog bronhitisa." Master's thesis, University of Zagreb, Faculty of Food Technology and Biotechnology, 2020. https://urn.nsk.hr/urn:nbn:hr:159:934593

Ćurčibašić, Valentina. "Optimizacija uzgoja virusa sličnog varijantu 2 u svrhu razvoja cjepiva protiv ptičjeg infektivnog bronhitisa." Master's thesis, University of Zagreb, Faculty of Food Technology and Biotechnology, 2020. https://urn.nsk.hr/urn:nbn:hr:159:934593

Ćurčibašić, V. (2020). 'Optimizacija uzgoja virusa sličnog varijantu 2 u svrhu razvoja cjepiva protiv ptičjeg infektivnog bronhitisa', Master's thesis, University of Zagreb, Faculty of Food Technology and Biotechnology, accessed 05 November 2024, https://urn.nsk.hr/urn:nbn:hr:159:934593

Ćurčibašić V. Optimizacija uzgoja virusa sličnog varijantu 2 u svrhu razvoja cjepiva protiv ptičjeg infektivnog bronhitisa [Master's thesis]. Zagreb: University of Zagreb, Faculty of Food Technology and Biotechnology; 2020 [cited 2024 November 05] Available at: https://urn.nsk.hr/urn:nbn:hr:159:934593

V. Ćurčibašić, "Optimizacija uzgoja virusa sličnog varijantu 2 u svrhu razvoja cjepiva protiv ptičjeg infektivnog bronhitisa", Master's thesis, University of Zagreb, Faculty of Food Technology and Biotechnology, Zagreb, 2020. Available at: https://urn.nsk.hr/urn:nbn:hr:159:934593

Metadata
TitleOptimizacija uzgoja virusa sličnog varijantu 2 u svrhu razvoja cjepiva protiv ptičjeg infektivnog bronhitisa
Title (english)Optimization of variant-2 like virus cultivation for the development of avian infectious bronchitis vaccine
AuthorValentina Ćurčibašić
MentorBlaženka Kos (mentor)
Committee memberJagoda Šušković (predsjednik povjerenstva)
GranterUniversity of Zagreb
Faculty of Food Technology and Biotechnology
(Department of Biochemical Engineering)
(Laboratory for Antibiotic, Enzyme, Probiotic and Starter Cultures Technology)
Zagreb
Defense date and country2020-07-01, Croatia
Scientific / art field,
discipline and subdiscipline		BIOTECHNICAL SCIENCES
Biotechnology
Abstract
Ptičji infektivni bronhitis je bolest koja uzrokuje probleme u peradarskoj industriji diljem svijeta. Bolest napada respiratorni sustav pilića čime izaziva velike ekonomske gubitke u peradarskoj industriji. Uzročnik bolesti je virus infektivnog bronhitisa koji je podložan čestim mutacijama i genomskim rekombinacijama. Zbog širokog spektra različitih serotipova i genotipova virusa potrebno je razvijati nova cjepiva. Optimizacija uzgoja živog cjepiva, glavnog alata u kontroli širenja infektivnog bronhitisa, soja virusa sličnog varijantu 2, ispitana je metodom dizajna eksperimenta. U istraživanje su bila uključena tri različita čimbenika: starost embrija u SPF jajima, veličina inokuluma i trajanje inkubacije. Količina virusa izražava se kao logaritam recipročnog razrjeđenja doze koja izaziva 50% promjena na inficiranoj staničnoj kulturi. Zajednički učinak primijenjenog titra inokuluma i trajanja inkubacije je imao najveći utjecaj na postignuti titar virusa sličnog varijantu 2.
Abstract (english)
The avian infectious bronchitis is disease that causes problems in poultry industry. A disease affecting the respiratory system of chickens and is associated with huge economic losses in poultry industry. Avian infectious bronchitis is caused by infectious bronchitis virus with great ability for mutation and genomic recombination. Because of many different serotypes and genotypes of virus the development of new vaccines is necessary. Optimization of variant 2-like virus cultivation as live vaccine, the most important method for controlling infectious bronchitis, was performed by the experimental design methodology. The experiments were performed by studying the effects of three different factors: the age of the embryos, the initial inoculum and the duration of virus incubation. The amount of virus was expressed as the logarithm of reciprocal dose dilution causing 50% change of infected cells. The greatest effect on the variant 2-like virus titer was achieved by the combined effect of the inoculum titer and incubation time.
Keywords
Keywords (english)
Languagecroatian
URN:NBNurn:nbn:hr:159:934593
Study programmeTitle: Molecular Biotechnology
Study programme type: university
Study level: graduate
Academic / professional title: magistar/magistra inženjer/inženjerka molekularne biotehnologije (magistar/magistra inženjer/inženjerka molekularne biotehnologije)
Type of resourceText
File originBorn digital
Access conditionsOpen access
Terms of useLicence Attribution-NoDerivatives 4.0 International (CC BY-ND 4.0) icon
Created on2020-07-01 10:12:04