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master's thesis
Optimizacija hranjive podloge za održavanje bakterija roda Bacteroides u in vitro uvjetima
Zagreb: University of Zagreb, Faculty of Food Technology and Biotechnology, 2020. urn:nbn:hr:159:088128
Galić, Sanja
University of Zagreb
Faculty of Food Technology and Biotechnology
Department of Biochemical Engineering
Section for Bioinformatics

Institutional repository: Repository of the Faculty of Food Technology and Biotechnology

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Galić, S. (2020). Optimizacija hranjive podloge za održavanje bakterija roda Bacteroides u in vitro uvjetima (Master's thesis). Zagreb: University of Zagreb, Faculty of Food Technology and Biotechnology. Retrieved from https://urn.nsk.hr/urn:nbn:hr:159:088128

Galić, Sanja. "Optimizacija hranjive podloge za održavanje bakterija roda Bacteroides u in vitro uvjetima." Master's thesis, University of Zagreb, Faculty of Food Technology and Biotechnology, 2020. https://urn.nsk.hr/urn:nbn:hr:159:088128

Galić, Sanja. "Optimizacija hranjive podloge za održavanje bakterija roda Bacteroides u in vitro uvjetima." Master's thesis, University of Zagreb, Faculty of Food Technology and Biotechnology, 2020. https://urn.nsk.hr/urn:nbn:hr:159:088128

Galić, S. (2020). 'Optimizacija hranjive podloge za održavanje bakterija roda Bacteroides u in vitro uvjetima', Master's thesis, University of Zagreb, Faculty of Food Technology and Biotechnology, accessed 23 November 2024, https://urn.nsk.hr/urn:nbn:hr:159:088128

Galić S. Optimizacija hranjive podloge za održavanje bakterija roda Bacteroides u in vitro uvjetima [Master's thesis]. Zagreb: University of Zagreb, Faculty of Food Technology and Biotechnology; 2020 [cited 2024 November 23] Available at: https://urn.nsk.hr/urn:nbn:hr:159:088128

S. Galić, "Optimizacija hranjive podloge za održavanje bakterija roda Bacteroides u in vitro uvjetima", Master's thesis, University of Zagreb, Faculty of Food Technology and Biotechnology, Zagreb, 2020. Available at: https://urn.nsk.hr/urn:nbn:hr:159:088128

Metadata
TitleOptimizacija hranjive podloge za održavanje bakterija roda Bacteroides u in vitro uvjetima
Title (english)Optimization of growth medium for maintaining bacteria of genus Bacteroides in vitro
AuthorSanja Galić
MentorJurica Žučko (mentor)
Committee memberJanko Diminić (predsjednik povjerenstva)
GranterUniversity of Zagreb
Faculty of Food Technology and Biotechnology
(Department of Biochemical Engineering)
(Section for Bioinformatics)
Zagreb
Defense date and country2020-07-15, Croatia
Scientific / art field,
discipline and subdiscipline		BIOTECHNICAL SCIENCES
Nutrition
Abstract
Zbog važnosti utjecaja na zdravlje čovjeka crijevna mikrobiota postala je često područje istraživanja. Budući da je provođenje istraživanja na ljudima iznimno kompleksno, sve se više razvijaju in vitro modeli za simulaciju uvjeta u ljudskome probavnom sustavu. Svrha ovog rada bila je u već razvijenom in vitro sustavu testirati utjecaj SIEM (eng. Simulated Illeal Efflux Medium) hranjive podloge na održavanje sastava inokulirane crijevne mikrobiote. Uzgoj crijevne mikrobiote u in vitro sustavu korištenjem SIEM hranjive podloge rezultirao je drastičnim padom udjela pripadnika koljena Bacteroidetes, te je predložena optimizacija hranjive podloge na osnovi podloga koje se koriste za uzgoj bakterija koje pripadaju rodu Bacteroides – najzastupljenijem rodu koljena Bacteroidetes u crijevnoj mikrobioti čovjeka.
Abstract (english)
Due to its importance for human health, the intestinal microbiota has become popular topic of research. Since conducting research on humans is demanding and complex new in vitro models are increasingly being developed to simulate conditions in the human gastrointestinal tract. The aim of this thesis was to test the influence of SIEM (Simulated Illeal Efflux Medium) growth medium on the viability of the inoculated microbiota composition in previously developed in vitro system simulating lower gastrointestinal tract. Cultivation of intestinal microbiota in vitro using the SIEM medium had resulted in drastic decline of members of the Bacteroidetes phylum. Optimization of SIEM growth media was proposed based on growth media used for cultivating bacteria of genus Bacteroides - most abundant genus of Bacteroidetes phylum in human gut microbiota.
Keywords
Keywords (english)
Languagecroatian
URN:NBNurn:nbn:hr:159:088128
Study programmeTitle: Nutrition
Study programme type: university
Study level: graduate
Academic / professional title: magistar/magistra nutricionizma (magistar/magistra nutricionizma)
Type of resourceText
File originBorn digital
Access conditionsOpen access
Terms of useLicence Attribution-NoDerivatives 4.0 International (CC BY-ND 4.0) icon
Created on2020-07-15 10:09:28