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master's thesis
Fotodinamička inaktivacija u bakterije Escherichia coli
Zagreb: University of Zagreb, Faculty of Food Technology and Biotechnology, 2014. urn:nbn:hr:159:941055
Gašpar, Nataša
University of Zagreb
Faculty of Food Technology and Biotechnology
Department of Biochemical Engineering
Section for Bioinformatics

Institutional repository: Repository of the Faculty of Food Technology and Biotechnology

Cite this document

Gašpar, N. (2014). Fotodinamička inaktivacija u bakterije Escherichia coli (Master's thesis). Zagreb: University of Zagreb, Faculty of Food Technology and Biotechnology. Retrieved from https://urn.nsk.hr/urn:nbn:hr:159:941055

Gašpar, Nataša. "Fotodinamička inaktivacija u bakterije Escherichia coli." Master's thesis, University of Zagreb, Faculty of Food Technology and Biotechnology, 2014. https://urn.nsk.hr/urn:nbn:hr:159:941055

Gašpar, Nataša. "Fotodinamička inaktivacija u bakterije Escherichia coli." Master's thesis, University of Zagreb, Faculty of Food Technology and Biotechnology, 2014. https://urn.nsk.hr/urn:nbn:hr:159:941055

Gašpar, N. (2014). 'Fotodinamička inaktivacija u bakterije Escherichia coli', Master's thesis, University of Zagreb, Faculty of Food Technology and Biotechnology, accessed 24 December 2024, https://urn.nsk.hr/urn:nbn:hr:159:941055

Gašpar N. Fotodinamička inaktivacija u bakterije Escherichia coli [Master's thesis]. Zagreb: University of Zagreb, Faculty of Food Technology and Biotechnology; 2014 [cited 2024 December 24] Available at: https://urn.nsk.hr/urn:nbn:hr:159:941055

N. Gašpar, "Fotodinamička inaktivacija u bakterije Escherichia coli", Master's thesis, University of Zagreb, Faculty of Food Technology and Biotechnology, Zagreb, 2014. Available at: https://urn.nsk.hr/urn:nbn:hr:159:941055

Metadata
TitleFotodinamička inaktivacija u bakterije Escherichia coli
Title (english)Photodynamic inactivation in Escherichia coli
AuthorNataša Gašpar
MentorAntonio Starčević (mentor)
GranterUniversity of Zagreb
Faculty of Food Technology and Biotechnology
(Department of Biochemical Engineering)
(Section for Bioinformatics)
Zagreb
Defense date and country2014-12-16, Croatia
Scientific / art field,
discipline and subdiscipline		BIOTECHNICAL SCIENCES
Biotechnology
Abstract
Cilj ovoga rada je bio analizirati E.coli K-12 mutante u fotodinamičkoj inaktivaciji. Korišteni sojevi potječu iz Keio kolekcije i imaju inaktiviran jedan gen za sintezu dijela lipopolisaharidnog omotača (rfaC, rfaE, lpcA). Glavni cilj rada je bila konstrukcija duplih mutanata koji imaju u isto vrijeme inaktiviran za jedan od spomenutih gena i inaktivaciju za transportni protein TolC. Dotični geni su se pokazali posebno osetljivima u fotodinamičkoj inaktivaciji s klorinom E6. Budući da spomenuti mutanti imaju fenotip ˝deep rough˝ i ne mogu biti inficirani s P1 bakteriofagom, standardna genetička transdukcija posredovana P1 bakteriofagom se ne može koristiti za konstrukciju mutanata, već alternativna metoda bazirana na sustavu λ-crvene rekombinaze. Koristeći dobivene rezultate, odnos između bakterijske stanične površine (lipopolisaharidni omotač) i njegov utjecaj na preživljavanje u fotodinamičkoj inaktivaciji, trebao bi biti razjašnjen. Rezultati ovoga rada trebali bi unaprijediti metodu fotodinamičke inaktivacije u borbi protiv gram-negativnih bakterija.
Abstract (english)
The aim of this thesis is to analyze lipopolysaccharide (LPS) E. coli K-12 mutants in photodynamic inactivation (PDI). The used strains are from the Keio collection and have single knockout genes for the lipopolysaccharide bilayer (rfaC, rfaE, lpcA). Goal of this thesis was to create double knockout mutants which have a knockout for one of these mentioned LPS genes and at the same time a tolC deletion. Those three genes are particularly sensitive in PDI with chlorine E6 (CE6). Since the LPS mutants have a ˝deep rugh˝ phenotype and can`t be infected with P1 bacteriophage, the standard genetic transduction couldn`t be used and different methods had to be performed, the λ-red recombinase system. Using the obtained results, the relationship between the composition of the bacterial cell surface and the survival in the PDI should be clarified. The results of this work should improve the PDI method against the Gram-negative bacteria.
Keywords
Keywords (english)
Languageenglish
URN:NBNurn:nbn:hr:159:941055
Study programmeTitle: Molecular Biotechnology
Study programme type: university
Study level: graduate
Academic / professional title: magistar/magistra inženjer/inženjerka molekularne biotehnologije (magistar/magistra inženjer/inženjerka molekularne biotehnologije)
Type of resourceText
File originBorn digital
Access conditionsAccess restricted to students and staff of home institution
Terms of useLicence In copyright icon
Created on2017-04-26 12:15:57