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undergraduate thesis
Usporedba metoda za obilježavanje i detekciju pri hibridizaciji DNA po Southern-u
Zagreb: University of Zagreb, Faculty of Food Technology and Biotechnology, 2020. urn:nbn:hr:159:161534
Lač, Maša
University of Zagreb
Faculty of Food Technology and Biotechnology
Department of Biochemical Engineering
Laboratory for Biology and Microbial Genetics

Institutional repository: Repository of the Faculty of Food Technology and Biotechnology

Cite this document

Lač, M. (2020). Usporedba metoda za obilježavanje i detekciju pri hibridizaciji DNA po Southern-u (Undergraduate thesis). Zagreb: University of Zagreb, Faculty of Food Technology and Biotechnology. Retrieved from https://urn.nsk.hr/urn:nbn:hr:159:161534

Lač, Maša. "Usporedba metoda za obilježavanje i detekciju pri hibridizaciji DNA po Southern-u." Undergraduate thesis, University of Zagreb, Faculty of Food Technology and Biotechnology, 2020. https://urn.nsk.hr/urn:nbn:hr:159:161534

Lač, Maša. "Usporedba metoda za obilježavanje i detekciju pri hibridizaciji DNA po Southern-u." Undergraduate thesis, University of Zagreb, Faculty of Food Technology and Biotechnology, 2020. https://urn.nsk.hr/urn:nbn:hr:159:161534

Lač, M. (2020). 'Usporedba metoda za obilježavanje i detekciju pri hibridizaciji DNA po Southern-u', Undergraduate thesis, University of Zagreb, Faculty of Food Technology and Biotechnology, accessed 15 July 2024, https://urn.nsk.hr/urn:nbn:hr:159:161534

Lač M. Usporedba metoda za obilježavanje i detekciju pri hibridizaciji DNA po Southern-u [Undergraduate thesis]. Zagreb: University of Zagreb, Faculty of Food Technology and Biotechnology; 2020 [cited 2024 July 15] Available at: https://urn.nsk.hr/urn:nbn:hr:159:161534

M. Lač, "Usporedba metoda za obilježavanje i detekciju pri hibridizaciji DNA po Southern-u", Undergraduate thesis, University of Zagreb, Faculty of Food Technology and Biotechnology, Zagreb, 2020. Available at: https://urn.nsk.hr/urn:nbn:hr:159:161534

Metadata
TitleUsporedba metoda za obilježavanje i detekciju pri hibridizaciji DNA po Southern-u
Title (english)Comparison of methods for DNA labelling and detection used in Southern blot hybridization
AuthorMaša Lač
MentorAnamarija Štafa (mentor)
GranterUniversity of Zagreb
Faculty of Food Technology and Biotechnology
(Department of Biochemical Engineering)
(Laboratory for Biology and Microbial Genetics)
Zagreb
Defense date and country2020-07-10, Croatia
Scientific / art field,
discipline and subdiscipline		BIOTECHNICAL SCIENCES
Biotechnology
Abstract
Hibridizacija po Southern-u koristi se za detekciju sekvencija DNA, a temelji se na komplementarnom sparivanju (hibridizaciji) jednolančane obilježene DNA sonde s jednolančanom ciljnom sekvencijom DNA na membrani. Hibridizacija se odvija pri uvjetima koji omogućuju komplementarno sparivanje DNA sonde s ciljnom regijom, uz minimalnu količinu nespecifičnog vezanja sonde na membranu. DNA sonde mogu se obilježavati radioaktivno, koristeći radioizotope (³²P, ³⁵S, ¹²⁵I, ³H) ili neradioaktivno, koristeći specifične molekule poput biotina ili digoksigenina (DIG). Najčešće korištena metoda za detekciju radioaktivno obilježenih sondi je autoradiografija, dok se detekcija neradioaktivno obilježenih sondi temelji na kemiluminiscencijskim i kromogenim reakcijama. U ovom radu uspoređene su različite metode obilježavanja i detekcije pri hibridizaciji po Southern-u te su navedene njihove prednosti i mane.
Abstract (english)
Southern blot is a method used for detection of specific DNA sequences and it is based on the complementary pairing of a labelled single-stranded DNA probe with target single-stranded DNA bound to the membrane. Hybridization occurs under stringent conditions which allow specific binding of a probe with a target sequence with the minimal nonspecific binding of DNA probe to the membrane. Probes can be labelled radioactively, using radioisotopes (³²P, ³⁵S, ¹²⁵I, ³H) or non-radioactively, using specific biotin or digoxigenin (DIG) molecules. Most commonly used detection method for radiolabeled probes is autoradiography, while detection of non-radioactive methods is based on chemiluminescence and chromogenic reactions. In this thesis, different methods for labelling and detection during Southern blot hybridization were compared and their advantages and disadvantages are indicated.
Keywords
Keywords (english)
Languagecroatian
URN:NBNurn:nbn:hr:159:161534
Study programmeTitle: Biotechnology
Study programme type: university
Study level: undergraduate
Academic / professional title: sveučilišni/a prvostupnik/prvostupnica (baccalaureus/baccalaurea) inženjer/inženjerka biotehnologije (sveučilišni/a prvostupnik/prvostupnica (baccalaureus/baccalaurea) inženjer/inženjerka biotehnologije)
Type of resourceText
File originBorn digital
Access conditionsOpen access
Terms of useLicence Attribution-NoDerivatives 4.0 International (CC BY-ND 4.0) icon
Created on2020-07-27 09:35:02