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undergraduate thesis
Metode genetičke transformacije kvasaca
Zagreb: University of Zagreb, Faculty of Food Technology and Biotechnology, 2020. urn:nbn:hr:159:513574
Jurić, Gabrijela
University of Zagreb
Faculty of Food Technology and Biotechnology
Department of Biochemical Engineering
Laboratory for Biology and Microbial Genetics

Institutional repository: Repository of the Faculty of Food Technology and Biotechnology

Cite this document

Jurić, G. (2020). Metode genetičke transformacije kvasaca (Undergraduate thesis). Zagreb: University of Zagreb, Faculty of Food Technology and Biotechnology. Retrieved from https://urn.nsk.hr/urn:nbn:hr:159:513574

Jurić, Gabrijela. "Metode genetičke transformacije kvasaca." Undergraduate thesis, University of Zagreb, Faculty of Food Technology and Biotechnology, 2020. https://urn.nsk.hr/urn:nbn:hr:159:513574

Jurić, Gabrijela. "Metode genetičke transformacije kvasaca." Undergraduate thesis, University of Zagreb, Faculty of Food Technology and Biotechnology, 2020. https://urn.nsk.hr/urn:nbn:hr:159:513574

Jurić, G. (2020). 'Metode genetičke transformacije kvasaca', Undergraduate thesis, University of Zagreb, Faculty of Food Technology and Biotechnology, accessed 15 July 2024, https://urn.nsk.hr/urn:nbn:hr:159:513574

Jurić G. Metode genetičke transformacije kvasaca [Undergraduate thesis]. Zagreb: University of Zagreb, Faculty of Food Technology and Biotechnology; 2020 [cited 2024 July 15] Available at: https://urn.nsk.hr/urn:nbn:hr:159:513574

G. Jurić, "Metode genetičke transformacije kvasaca", Undergraduate thesis, University of Zagreb, Faculty of Food Technology and Biotechnology, Zagreb, 2020. Available at: https://urn.nsk.hr/urn:nbn:hr:159:513574

Metadata
TitleMetode genetičke transformacije kvasaca
Title (english)Yeasts transformation methods
AuthorGabrijela Jurić
MentorIvan-Krešimir Svetec (mentor)
GranterUniversity of Zagreb
Faculty of Food Technology and Biotechnology
(Department of Biochemical Engineering)
(Laboratory for Biology and Microbial Genetics)
Zagreb
Defense date and country2020-09-15, Croatia
Scientific / art field,
discipline and subdiscipline		BIOTECHNICAL SCIENCES
Interdisciplinary Technical Sciences
Abstract
Pod pojmom genetička transformacija podrazumijeva se laboratorijski postupak unošenja DNA u neku stanicu što rezultira promjenom genotipa i fenotipa transformirane stanice. Ovaj postupak se koristi u genetičkom inženjerstvu, a od iznimnog je značaja u biotehnologiji jer omogućava konstrukciju sojeva koji se koriste kao radni organizmi u biotehnološkim procesima. U ovom radu diskutirane su prednosti i mane metoda koje se najčešće koriste za transformaciju kvasaca kao što su metoda protoplastiranja, metoda pomoću litijevog acetata, elektroporacija, metoda pomoću staklenih kuglica, biolistička transformacija te transformacija pomoću bakterije Agrobacterium tumefaciens. Osim toga dane su najvažnije karakteristike vektora (molekula DNA) koje se koriste za ekspresiju heterolognih proteina i modifikaciju kvaščevog genoma te karakteristike pojedinih nekonvencionalnih kvasaca i primjeri njihove primjene u biotehnologiji.
Abstract (english)
The term genetic transformation refers to a laboratory procedure of introducing DNA into a cell, which results in a change of the genotype and phenotype of the transformed cell. This procedure has been used in genetic engineering, and is of great importance in biotechnology because it allows the construction of strains, which are used as working organisms in biotechnological processes. This paper discusses the advantages and disadvantages of the commonly used yeast transformation methods such as spheroplast method, lithium acetate method, electroporation, glass beads method, biolistic transformation and Agrobacterium tumefaciens mediated transformation. In addition, the most important characteristics of vectors (DNA molecules) used for the expression of heterologous proteins and modification of the yeast genome are given, as well as the characteristics of several unconventional yeasts and examples of their application in biotechnology.
Keywords
Keywords (english)
Languagecroatian
URN:NBNurn:nbn:hr:159:513574
Study programmeTitle: Biotechnology
Study programme type: university
Study level: undergraduate
Academic / professional title: sveučilišni/a prvostupnik/prvostupnica (baccalaureus/baccalaurea) inženjer/inženjerka biotehnologije (sveučilišni/a prvostupnik/prvostupnica (baccalaureus/baccalaurea) inženjer/inženjerka biotehnologije)
Type of resourceText
File originBorn digital
Access conditionsOpen access
Terms of useLicence Attribution-NoDerivatives 4.0 International (CC BY-ND 4.0) icon
Created on2020-10-14 10:26:24