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undergraduate thesis
Proizvodnja i izdvajanje ksilitola iz hidrolizata otpadne trave
Zagreb: University of Zagreb, Faculty of Food Technology and Biotechnology, 2020. urn:nbn:hr:159:143665
Lilek, Stela
University of Zagreb
Faculty of Food Technology and Biotechnology
Department of Biochemical Engineering
Laboratory for Biochemical Engineering, Industrial Microbiology and Malting and Brewing Technology

Institutional repository: Repository of the Faculty of Food Technology and Biotechnology

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Lilek, S. (2020). Proizvodnja i izdvajanje ksilitola iz hidrolizata otpadne trave (Undergraduate thesis). Zagreb: University of Zagreb, Faculty of Food Technology and Biotechnology. Retrieved from https://urn.nsk.hr/urn:nbn:hr:159:143665

Lilek, Stela. "Proizvodnja i izdvajanje ksilitola iz hidrolizata otpadne trave." Undergraduate thesis, University of Zagreb, Faculty of Food Technology and Biotechnology, 2020. https://urn.nsk.hr/urn:nbn:hr:159:143665

Lilek, Stela. "Proizvodnja i izdvajanje ksilitola iz hidrolizata otpadne trave." Undergraduate thesis, University of Zagreb, Faculty of Food Technology and Biotechnology, 2020. https://urn.nsk.hr/urn:nbn:hr:159:143665

Lilek, S. (2020). 'Proizvodnja i izdvajanje ksilitola iz hidrolizata otpadne trave', Undergraduate thesis, University of Zagreb, Faculty of Food Technology and Biotechnology, accessed 20 November 2024, https://urn.nsk.hr/urn:nbn:hr:159:143665

Lilek S. Proizvodnja i izdvajanje ksilitola iz hidrolizata otpadne trave [Undergraduate thesis]. Zagreb: University of Zagreb, Faculty of Food Technology and Biotechnology; 2020 [cited 2024 November 20] Available at: https://urn.nsk.hr/urn:nbn:hr:159:143665

S. Lilek, "Proizvodnja i izdvajanje ksilitola iz hidrolizata otpadne trave", Undergraduate thesis, University of Zagreb, Faculty of Food Technology and Biotechnology, Zagreb, 2020. Available at: https://urn.nsk.hr/urn:nbn:hr:159:143665

Metadata
TitleProizvodnja i izdvajanje ksilitola iz hidrolizata otpadne trave
Title (english)Production and extraction of xylitol from waste grass hydrolysates
AuthorStela Lilek
MentorAntonija Trontel (mentor)
GranterUniversity of Zagreb
Faculty of Food Technology and Biotechnology
(Department of Biochemical Engineering)
(Laboratory for Biochemical Engineering, Industrial Microbiology and Malting and Brewing Technology)
Zagreb
Defense date and country2020-09-01, Croatia
Scientific / art field,
discipline and subdiscipline		BIOTECHNICAL SCIENCES
Biotechnology
Abstract
Ksilitol je industrijski važan poliol koji se zbog svojih svojstava koristi kao zamjena za šećer te se dodaje u različite proizvode. U ovom radu proveden je šaržni uzgoj plijesni Mucor indicus DSM 2185 na hidrolizatu otpadne trave s ciljem proizvodnje ksilitola. Nakon provedenog procesa proizvodnje ksilitola centrifugiranjem su izdvojeni biomasa i supernatant. Efikasnost procesa proizvodnje ksilitola iznosila je 42,16 % uz prinos ksilitola od 14,57 g Lˉ¹. Supernatant je korišten za izdvajanje ksilitola kristalizacijom. Maksimalni prinos procesa kristalizacije iznosio je oko 30%. Biomasa ove plijesni predstavlja važan nusproizvod, te je korištena za izolaciju hitina i hitozana. Nakon provedenog postupka izolacije hitina i hitozana, iz biomase plijesni dobiveno je 20,56% hitina te 9,90% hitozana određeno na suhu tvar. Dodatno, određen je i sastav masnih kiselina ove oleaginozne plijesni. Ukupni udio lipida u biomasi plijesni iznosi svega 5,90 % izraženo na suhu tvar, te je plinskom kromatografijom identificirano i kvantificirano 13 estera masnih kiselina, od kojih najveći udio imaju oleinska (C18:1 cis9), linolenska (C18:2 cis 9,12) i γ-linolenska kiselina (C18:3 cis 6,9,12).
Abstract (english)
Xylitol is an industrially important polyol which, due to its properties, is used as a substitute for sucrose and is added to various products. In this paper, batch cultivation of mold Mucor indicus DSM 2185, with the aim of xylitol production, on waste grass hydrolyzate was performed. After the xylitol production process, biomass and supernatant were separated by centrifugation. The efficiency of the xylitol production process was 42,16% with a xylitol yield of 14,57 g Lˉ¹. The supernatant was used to separate the xylitol by crystallization. The maximum yield of the crystallization process was about 30%. The biomass of this mold is an important by-product, and, therefore, has been used to isolate chitin and chitosan. After the isolation procedure of chitin and chitosan, 20.56% of chitin and 9.90% of chitosan was isolated from biomass. Additionally, the fatty acid composition of this oleaginous mold was determined. The total lipid content in biomass was only 5.90%. Gas chromatographic method was used for identification and quantification of 13 fatty acid esters, from which oleic (C18: 1 cis9), linolenic (C18: 2 cis 9, 12) and γ-linolenic acid (C18: 3 cis 6,9,12) have highest concentration.
Keywords
Keywords (english)
Languagecroatian
URN:NBNurn:nbn:hr:159:143665
Study programmeTitle: Biotechnology
Study programme type: university
Study level: undergraduate
Academic / professional title: sveučilišni/a prvostupnik/prvostupnica (baccalaureus/baccalaurea) inženjer/inženjerka biotehnologije (sveučilišni/a prvostupnik/prvostupnica (baccalaureus/baccalaurea) inženjer/inženjerka biotehnologije)
Type of resourceText
File originBorn digital
Access conditionsOpen access
Terms of useLicence Attribution-NoDerivatives 4.0 International (CC BY-ND 4.0) icon
Created on2020-10-14 12:41:00