prikaz prve stranice dokumenta Restrikcijska analiza plazmida pRS40cN, pRS52cN, pRS53cN i pRS55cN
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undergraduate thesis
Restrikcijska analiza plazmida pRS40cN, pRS52cN, pRS53cN i pRS55cN
Zagreb: University of Zagreb, Faculty of Food Technology and Biotechnology, 2020. urn:nbn:hr:159:057005
Slišković, Ana
University of Zagreb
Faculty of Food Technology and Biotechnology
Department of Biochemical Engineering
Laboratory for Biology and Microbial Genetics

Institutional repository: Repository of the Faculty of Food Technology and Biotechnology

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Slišković, A. (2020). Restrikcijska analiza plazmida pRS40cN, pRS52cN, pRS53cN i pRS55cN (Undergraduate thesis). Zagreb: University of Zagreb, Faculty of Food Technology and Biotechnology. Retrieved from https://urn.nsk.hr/urn:nbn:hr:159:057005

Slišković, Ana. "Restrikcijska analiza plazmida pRS40cN, pRS52cN, pRS53cN i pRS55cN." Undergraduate thesis, University of Zagreb, Faculty of Food Technology and Biotechnology, 2020. https://urn.nsk.hr/urn:nbn:hr:159:057005

Slišković, Ana. "Restrikcijska analiza plazmida pRS40cN, pRS52cN, pRS53cN i pRS55cN." Undergraduate thesis, University of Zagreb, Faculty of Food Technology and Biotechnology, 2020. https://urn.nsk.hr/urn:nbn:hr:159:057005

Slišković, A. (2020). 'Restrikcijska analiza plazmida pRS40cN, pRS52cN, pRS53cN i pRS55cN', Undergraduate thesis, University of Zagreb, Faculty of Food Technology and Biotechnology, accessed 02 December 2024, https://urn.nsk.hr/urn:nbn:hr:159:057005

Slišković A. Restrikcijska analiza plazmida pRS40cN, pRS52cN, pRS53cN i pRS55cN [Undergraduate thesis]. Zagreb: University of Zagreb, Faculty of Food Technology and Biotechnology; 2020 [cited 2024 December 02] Available at: https://urn.nsk.hr/urn:nbn:hr:159:057005

A. Slišković, "Restrikcijska analiza plazmida pRS40cN, pRS52cN, pRS53cN i pRS55cN", Undergraduate thesis, University of Zagreb, Faculty of Food Technology and Biotechnology, Zagreb, 2020. Available at: https://urn.nsk.hr/urn:nbn:hr:159:057005

Metadata
TitleRestrikcijska analiza plazmida pRS40cN, pRS52cN, pRS53cN i pRS55cN
Title (english)Restriction analysis of plasmids pRS40cN, pRS52cN, pRS53cN and pRS55cN
AuthorAna Slišković
MentorIvan-Krešimir Svetec (mentor)
GranterUniversity of Zagreb
Faculty of Food Technology and Biotechnology
(Department of Biochemical Engineering)
(Laboratory for Biology and Microbial Genetics)
Zagreb
Defense date and country2020-09-01, Croatia
Scientific / art field,
discipline and subdiscipline		BIOTECHNICAL SCIENCES
Biotechnology
Abstract
Pod pojmom restrikcijska analiza, u molekularnoj genetici i genetičkom inženjerstvu, podrazumijeva se analiza DNA korištenjem restrikcijskih enzima, odnosno endonukleaza koje cijepaju DNA u točno određenim sekvencijama. Stoga su ovi enzimi nezamjenjivi u genetičkom inženjerstvu i mnogim molekularno-genetičkim metodama koje imaju primjenu u dijagnostici, filogeniji i identifikaciji organizama. U ovom radu je restrikcijskom analizom potvrđena struktura prethodno konstruiranih plazmida pRS40cN, pRS52cN, pRS53cN i pRS55cN. U tu su svrhu plazmidi najprije izolirani i pročišćeni iz velikih volumena kultura bakterije Eschericha coli, a zatim pocijepani odgovarajućim restrikcijskim enzimima te podvrgnuti elektroforezi u agaroznom gelu. Na temelju rezultata analize potvrđeno je da restrikcijske mape svih plazmida odgovaraju njihovoj stvarnoj strukturi.
Abstract (english)
In molecular genetics and genetic engineering, the term restriction analysis refers to the analysis of DNA molecules using restriction enzymes, endonucleases that cleave DNA in specific nucleotide sequences. Therefore, these enzymes are indispensable in genetic engineering, as well as in many molecular genetic methods that are used in diagnostics, phylogeny and identification of organisms. In this paper, restriction analysis was used to confirm the structure of previously constructed plasmids pRS40cN, pRS52cN, pRS53cN and pRS55cN. For this purpose, the plasmids were first isolated and purified from large volumes of Escherichia coli cultures, then digested with appropriate restriction enzymes and subjected to agarose gel electrophoresis. Based on the results of the analysis, it was confirmed that the restriction maps of all plasmids correspond to their actual structure.
Keywords
Keywords (english)
Languagecroatian
URN:NBNurn:nbn:hr:159:057005
Study programmeTitle: Biotechnology
Study programme type: university
Study level: undergraduate
Academic / professional title: sveučilišni/a prvostupnik/prvostupnica (baccalaureus/baccalaurea) inženjer/inženjerka biotehnologije (sveučilišni/a prvostupnik/prvostupnica (baccalaureus/baccalaurea) inženjer/inženjerka biotehnologije)
Type of resourceText
File originBorn digital
Access conditionsOpen access
Terms of useLicence Attribution-NoDerivatives 4.0 International (CC BY-ND 4.0) icon
Created on2020-10-16 11:22:39