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undergraduate thesis
Primjena eutektičkih otapala u reakcijama kataliziranim alkohol dehidrogenazom
Zagreb: University of Zagreb, Faculty of Food Technology and Biotechnology, 2020. urn:nbn:hr:159:286923
Biškup, Marija
University of Zagreb
Faculty of Food Technology and Biotechnology
Department of Biochemical Engineering
Laboratory for Cell Technology, Application and Biotransformations

Institutional repository: Repository of the Faculty of Food Technology and Biotechnology

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Biškup, M. (2020). Primjena eutektičkih otapala u reakcijama kataliziranim alkohol dehidrogenazom (Undergraduate thesis). Zagreb: University of Zagreb, Faculty of Food Technology and Biotechnology. Retrieved from https://urn.nsk.hr/urn:nbn:hr:159:286923

Biškup, Marija. "Primjena eutektičkih otapala u reakcijama kataliziranim alkohol dehidrogenazom." Undergraduate thesis, University of Zagreb, Faculty of Food Technology and Biotechnology, 2020. https://urn.nsk.hr/urn:nbn:hr:159:286923

Biškup, Marija. "Primjena eutektičkih otapala u reakcijama kataliziranim alkohol dehidrogenazom." Undergraduate thesis, University of Zagreb, Faculty of Food Technology and Biotechnology, 2020. https://urn.nsk.hr/urn:nbn:hr:159:286923

Biškup, M. (2020). 'Primjena eutektičkih otapala u reakcijama kataliziranim alkohol dehidrogenazom', Undergraduate thesis, University of Zagreb, Faculty of Food Technology and Biotechnology, accessed 05 November 2024, https://urn.nsk.hr/urn:nbn:hr:159:286923

Biškup M. Primjena eutektičkih otapala u reakcijama kataliziranim alkohol dehidrogenazom [Undergraduate thesis]. Zagreb: University of Zagreb, Faculty of Food Technology and Biotechnology; 2020 [cited 2024 November 05] Available at: https://urn.nsk.hr/urn:nbn:hr:159:286923

M. Biškup, "Primjena eutektičkih otapala u reakcijama kataliziranim alkohol dehidrogenazom", Undergraduate thesis, University of Zagreb, Faculty of Food Technology and Biotechnology, Zagreb, 2020. Available at: https://urn.nsk.hr/urn:nbn:hr:159:286923

Metadata
TitlePrimjena eutektičkih otapala u reakcijama kataliziranim alkohol dehidrogenazom
Title (english)Application of deep eutectic solvents in alcohol dehydrogenase-catalyzed reactions
AuthorMarija Biškup
MentorMarina Cvjetko Bubalo (mentor)
GranterUniversity of Zagreb
Faculty of Food Technology and Biotechnology
(Department of Biochemical Engineering)
(Laboratory for Cell Technology, Application and Biotransformations)
Zagreb
Defense date and country2020-09-15, Croatia
Scientific / art field,
discipline and subdiscipline		BIOTECHNICAL SCIENCES
Biotechnology
Abstract
Zahvaljujući sposobnosti kataliziranja redukcija karbonilnih grupa u prokiralnim spojevima do kiralnih alkohola, koji su važni međuprodukti u proizvodnji lijekova i kemikalija, dehidrogenaze su enzimi od velike industrijske važnosti. U novije vrijeme cilj je industrijsku proizvodnju uskladiti s načelima zelene kemije, stoga se velike količine hlapljivih, zapaljivih i toksičnih organskih otapala nastoje zamijeniti sigurnijim otapalima za ljudsko zdravlje i okoliš. Nova generacija takvih otapala su eutektička otapala koje karakteriziraju niska hlapljivost, biorazgradivost i jednostavna sinteza. Cilj ovog rada bio je ispitati mogućnosti primjene eutektičkih otapala u reakcijama kataliziranim alkohol dehidrogenazom. Pripravljena su različita eutektička otapala, s kolin-kloridom u sastavu, u kojima je ispitana aktivnost te su praćene promjene u konformaciji enzima alkohol dehidrogenaze. U istim je otapalima također praćena stabilnost koenzima NADH i NAD+ koji su potrebni za aktivnost alkohol dehidrogenaze. Eutektička otapala na bazi kolin-klorida s ureom u sastavu najbolje su stabilizirala oba oblika nikotinamidnog koenzima, no niti jedno eutektičko otapalo nije se pokazalo kao povoljan medij za odvijanje enzimske reakcije alkohol dehidrogenaze.
Abstract (english)
Due to their ability to catalyze the reduction of carbonyl groups in prochiral compounds to chiral alcohols, which are important intermediates in the production of pharmaceuticals and chemicals, dehydrogenases are enzymes of great industrial importance. Recently, a tendency is to coordinate industrial production with the principles of green chemistry, so large amounts of volatile, flammable and toxic organic solvents are being replaced by solvents which are safer for human health and the environment. A new generation of such solvents is deep eutectic solvents characterized by low volatility, biodegradability, and easy synthesis. The aim of this study was to investigate the possibilities of using deep eutectic solvents in alcohol dehydrogenase-catalyzed reactions. Various choline-chloride based deep eutectic solvents were prepared in which enzyme activity was measured and changes in the conformation of the enzyme alcohol dehydrogenase were monitored. In the same solvents, the stability of the coenzymes NADH and NAD+ required for alcohol dehydrogenase activity was also monitored. Choline-chloride based deep eutectic solvents containing urea best stabilized both forms of nicotinamide coenzyme, but neither deep eutectic solvent proved to be a suitable medium for the enzymatic reaction of alcohol dehydrogenase.
Keywords
Keywords (english)
Languagecroatian
URN:NBNurn:nbn:hr:159:286923
Study programmeTitle: Biotechnology
Study programme type: university
Study level: undergraduate
Academic / professional title: sveučilišni/a prvostupnik/prvostupnica (baccalaureus/baccalaurea) inženjer/inženjerka biotehnologije (sveučilišni/a prvostupnik/prvostupnica (baccalaureus/baccalaurea) inženjer/inženjerka biotehnologije)
Type of resourceText
File originBorn digital
Access conditionsOpen access
Terms of useLicence Attribution-NoDerivatives 4.0 International (CC BY-ND 4.0) icon
Created on2020-10-22 13:24:13