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undergraduate thesis
Metoda slučajnog umnažanja polimorfne DNA (RAPD)
Zagreb: University of Zagreb, Faculty of Food Technology and Biotechnology, 2020. urn:nbn:hr:159:991774
Ladan, Paola
University of Zagreb
Faculty of Food Technology and Biotechnology
Department of Biochemical Engineering
Laboratory for Biology and Microbial Genetics

Institutional repository: Repository of the Faculty of Food Technology and Biotechnology

Cite this document

Ladan, P. (2020). Metoda slučajnog umnažanja polimorfne DNA (RAPD) (Undergraduate thesis). Zagreb: University of Zagreb, Faculty of Food Technology and Biotechnology. Retrieved from https://urn.nsk.hr/urn:nbn:hr:159:991774

Ladan, Paola. "Metoda slučajnog umnažanja polimorfne DNA (RAPD)." Undergraduate thesis, University of Zagreb, Faculty of Food Technology and Biotechnology, 2020. https://urn.nsk.hr/urn:nbn:hr:159:991774

Ladan, Paola. "Metoda slučajnog umnažanja polimorfne DNA (RAPD)." Undergraduate thesis, University of Zagreb, Faculty of Food Technology and Biotechnology, 2020. https://urn.nsk.hr/urn:nbn:hr:159:991774

Ladan, P. (2020). 'Metoda slučajnog umnažanja polimorfne DNA (RAPD)', Undergraduate thesis, University of Zagreb, Faculty of Food Technology and Biotechnology, accessed 02 December 2024, https://urn.nsk.hr/urn:nbn:hr:159:991774

Ladan P. Metoda slučajnog umnažanja polimorfne DNA (RAPD) [Undergraduate thesis]. Zagreb: University of Zagreb, Faculty of Food Technology and Biotechnology; 2020 [cited 2024 December 02] Available at: https://urn.nsk.hr/urn:nbn:hr:159:991774

P. Ladan, "Metoda slučajnog umnažanja polimorfne DNA (RAPD)", Undergraduate thesis, University of Zagreb, Faculty of Food Technology and Biotechnology, Zagreb, 2020. Available at: https://urn.nsk.hr/urn:nbn:hr:159:991774

Metadata
TitleMetoda slučajnog umnažanja polimorfne DNA (RAPD)
Title (english)Random Amplification of Polymorphic DNA (RAPD) method
AuthorPaola Ladan
MentorIvan-Krešimir Svetec (mentor)
GranterUniversity of Zagreb
Faculty of Food Technology and Biotechnology
(Department of Biochemical Engineering)
(Laboratory for Biology and Microbial Genetics)
Zagreb
Defense date and country2020-09-15, Croatia
Scientific / art field,
discipline and subdiscipline		BIOTECHNICAL SCIENCES
Biotechnology
Abstract
Danas su u primjeni mnoge metode koje se osnivaju na metodi PCR (Polymerase Chain Reaction, lančana reakcija polimerazom), a jedna od njih je RAPD-PCR ili RAPD (Random Amplification of Polymorphic DNA, metoda slučajnog umnažanja polimorfne DNA). Reakcijske otopine za provođenje metode PCR i metode RAPD sadrže iste komponente, ali uvjeti provođenja reakcije su različiti. Metoda PCR se koristi za umnažanje točno određenog dijela DNA iz nekog genoma, a metodom RAPD umnaža se veći broj različitih fragmenata DNA. Stoga se usporedbom RAPD profila (umnoženih fragmenata) može donijeti zaključak o srodnosti analiziranih organizama odnosno postojanju polimorfizama u njihovim genomima. Glavne prednosti metode su niska cijena i kratko vrijeme potrebno za provođenje analize, a mana je što jako male promjene u uvjetima reakcije mogu značajno utjecati na reproducibilnost rezultata analize.
Abstract (english)
Many methods based on PCR (Polymerase Chain Reaction) are being used nowadays. One of them is RAPD-PCR or RAPD (Random Amplification of Polymorphic DNA). PCR and RAPD reaction solutions consist of same components but conditions in which the reaction is carried out differ in these two methods. PCR is used to amplify a specific part of a DNA from a genome, while RAPD enables the amplification of many different DNA fragments. By comparing RAPD profiles (amplified products), a conclusion about similarity between different organisms or the existence of polymorphisms in their genomes can be made. Main advantages of the method are low price and short analysis time, while its disadvantage is the significant influence of reaction conditions on the reproducibility of results.
Keywords
Keywords (english)
Languagecroatian
URN:NBNurn:nbn:hr:159:991774
Study programmeTitle: Biotechnology
Study programme type: university
Study level: undergraduate
Academic / professional title: sveučilišni/a prvostupnik/prvostupnica (baccalaureus/baccalaurea) inženjer/inženjerka biotehnologije (sveučilišni/a prvostupnik/prvostupnica (baccalaureus/baccalaurea) inženjer/inženjerka biotehnologije)
Type of resourceText
File originBorn digital
Access conditionsOpen access
Terms of useLicence Attribution-NoDerivatives 4.0 International (CC BY-ND 4.0) icon
Created on2020-10-26 11:46:47