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undergraduate thesis
Modifikacija genoma kvasca u svrhu označavanja proteina Pir1, Pir3, Pir5 i Spr1 hemaglutininskom oznakom (HA)
Zagreb: University of Zagreb, Faculty of Food Technology and Biotechnology, 2020. urn:nbn:hr:159:578757
Škarupka, Matea
University of Zagreb
Faculty of Food Technology and Biotechnology
Department of Chemistry and Biochemistry
Laboratory for Biochemistry

Institutional repository: Repository of the Faculty of Food Technology and Biotechnology

Cite this document

Škarupka, M. (2020). Modifikacija genoma kvasca u svrhu označavanja proteina Pir1, Pir3, Pir5 i Spr1 hemaglutininskom oznakom (HA) (Undergraduate thesis). Zagreb: University of Zagreb, Faculty of Food Technology and Biotechnology. Retrieved from https://urn.nsk.hr/urn:nbn:hr:159:578757

Škarupka, Matea. "Modifikacija genoma kvasca u svrhu označavanja proteina Pir1, Pir3, Pir5 i Spr1 hemaglutininskom oznakom (HA)." Undergraduate thesis, University of Zagreb, Faculty of Food Technology and Biotechnology, 2020. https://urn.nsk.hr/urn:nbn:hr:159:578757

Škarupka, Matea. "Modifikacija genoma kvasca u svrhu označavanja proteina Pir1, Pir3, Pir5 i Spr1 hemaglutininskom oznakom (HA)." Undergraduate thesis, University of Zagreb, Faculty of Food Technology and Biotechnology, 2020. https://urn.nsk.hr/urn:nbn:hr:159:578757

Škarupka, M. (2020). 'Modifikacija genoma kvasca u svrhu označavanja proteina Pir1, Pir3, Pir5 i Spr1 hemaglutininskom oznakom (HA)', Undergraduate thesis, University of Zagreb, Faculty of Food Technology and Biotechnology, accessed 18 May 2024, https://urn.nsk.hr/urn:nbn:hr:159:578757

Škarupka M. Modifikacija genoma kvasca u svrhu označavanja proteina Pir1, Pir3, Pir5 i Spr1 hemaglutininskom oznakom (HA) [Undergraduate thesis]. Zagreb: University of Zagreb, Faculty of Food Technology and Biotechnology; 2020 [cited 2024 May 18] Available at: https://urn.nsk.hr/urn:nbn:hr:159:578757

M. Škarupka, "Modifikacija genoma kvasca u svrhu označavanja proteina Pir1, Pir3, Pir5 i Spr1 hemaglutininskom oznakom (HA)", Undergraduate thesis, University of Zagreb, Faculty of Food Technology and Biotechnology, Zagreb, 2020. Available at: https://urn.nsk.hr/urn:nbn:hr:159:578757

Metadata
TitleModifikacija genoma kvasca u svrhu označavanja proteina Pir1, Pir3, Pir5 i Spr1 hemaglutininskom oznakom (HA)
Title (english)Yeast genome modification for the purpose of labeling proteins Pir1, Pir3, Pir5 and Spr1 with hemagglutinin tag (HA)
AuthorMatea Škarupka
MentorIgor Stuparević (mentor)
GranterUniversity of Zagreb
Faculty of Food Technology and Biotechnology
(Department of Chemistry and Biochemistry)
(Laboratory for Biochemistry)
Zagreb
Defense date and country2020-07-01, Croatia
Scientific / art field,
discipline and subdiscipline		BIOTECHNICAL SCIENCES
Nutrition
Abstract
Kvasac Saccharomyces cerevisiae je modelni organizam u biokemijskim i genetičkim istraživanjima zbog sličnosti mnogih staničnih procesa sa procesima u višim eukariotima. Stanice kvasca obavija stanična stijenka građena od glukanske mreže, koju čine β-1,3 i β-1,6- glukan, hitin i manoproteini. Građa stanične stijenke mijenja se ovisno o staničnom ciklusu kvasca pa se tako razlikuje u mitozi i mejozi. Prelaskom iz mitoze u mejozu, a zatim i prilikom formiranja sporula, dolazi do značajnih promjena u strukturi stanične stijenke što je posljedica promijenjene ekspresije pojedinih proteina. Cilj ovog rada bio je obilježiti proteine stanične stijenke Pir1, Pir3, Pir5 i Spr1 hemaglutininskom oznakom kako bi olakšali njihovo praćenje u različitim fazama staničnog ciklusa.
Abstract (english)
Yeast Saccharomyces cerevisiae is a model organism in biochemical and genetic research due to the similarity of many cellular processes with those in higher eukaryotes. Yeast cells are surrounded by a cell wall built of a glucan network, made up of β-1,3 and β-1,6-glucan, chitin, and mannoproteins. The structure of the cell wall varies depending on the cell cycle of the yeast, so it differs in mitosis and meiosis. With the transition from mitosis to meiosis, and then spore formation, there are significant changes in cell wall structure as a result of altered expression of individual proteins. The aim of this study was to label cell wall proteins Pir1, Pir3, Pir5 and Spr1 with a hemagglutinin label to facilitate their monitoring at different stages of the cell cycle.
Keywords
Keywords (english)
Languagecroatian
URN:NBNurn:nbn:hr:159:578757
Study programmeTitle: Nutrition
Study programme type: university
Study level: undergraduate
Academic / professional title: sveučilišni/a prvostupnik/prvostupnica (baccalaureus/baccalaurea) nutricionizma (sveučilišni/a prvostupnik/prvostupnica (baccalaureus/baccalaurea) nutricionizma)
Type of resourceText
File originBorn digital
Access conditionsOpen access
Terms of useLicence Attribution-NoDerivatives 4.0 International (CC BY-ND 4.0) icon
Created on2020-10-27 14:48:32