The flavocytochrome cellobiose dehydrogenase (CDH) is a fungal redox enzyme involved in oxidative cellulose degradation. CDH is a versatile biocatalyst that can be used as biorecognition element in electrochemical lactose and glucose sensors or in biocatalysis. In thiswork the production of CDH from the fungus Phanerochaete sordida, a well-known basidiomycete with high lignin-degrading abilities, was studied. Significant secretion of CDH was found when the fungus was grown on cellulose as sole carbon source. However, production and purification is challenging due to the slow growth of the fungus and low abundance of the enzyme in crude supernatants (~2.6% w/w of total protein). Therefore, different cultivation strategies were examined in order to optimize enzyme yields. Research was done in conventional shaken flasks with and without baffles. Additionally, the WAVE bioreactorsystem consisting of a disposable cellbag placed on a rocking device was evaluated as alternative cultivation system for filamentous fungi. Fungal growth in the WAVE bioreactor was relatively fast because of high oxygen transfer rate and minimum shear stress for the mycelium. CDH from Phanerochaete sordida was successfully purified by a two-step chromatography procedure. Kinetic constants show excellent specific activity towards lactose and cellobiose, whereas almost no conversion of glucose is observed (KM>3 M), which makes the enzyme an interesting candidate for lactose or cellobiose biosensors.