prikaz prve stranice dokumenta Rast i produktivnost CHO DP-12 stanica u kemijski definiranim hranjivim medijima
  Download
PDF 491.81 KB
undergraduate thesis
Rast i produktivnost CHO DP-12 stanica u kemijski definiranim hranjivim medijima
Zagreb: University of Zagreb, Faculty of Food Technology and Biotechnology, 2020. urn:nbn:hr:159:258410
Jelić, Ana
University of Zagreb
Faculty of Food Technology and Biotechnology
Department of Biochemical Engineering
Laboratory for Cell Technology, Application and Biotransformations

Institutional repository: Repository of the Faculty of Food Technology and Biotechnology

Cite this document

Jelić, A. (2020). Rast i produktivnost CHO DP-12 stanica u kemijski definiranim hranjivim medijima (Undergraduate thesis). Zagreb: University of Zagreb, Faculty of Food Technology and Biotechnology. Retrieved from https://urn.nsk.hr/urn:nbn:hr:159:258410

Jelić, Ana. "Rast i produktivnost CHO DP-12 stanica u kemijski definiranim hranjivim medijima." Undergraduate thesis, University of Zagreb, Faculty of Food Technology and Biotechnology, 2020. https://urn.nsk.hr/urn:nbn:hr:159:258410

Jelić, Ana. "Rast i produktivnost CHO DP-12 stanica u kemijski definiranim hranjivim medijima." Undergraduate thesis, University of Zagreb, Faculty of Food Technology and Biotechnology, 2020. https://urn.nsk.hr/urn:nbn:hr:159:258410

Jelić, A. (2020). 'Rast i produktivnost CHO DP-12 stanica u kemijski definiranim hranjivim medijima', Undergraduate thesis, University of Zagreb, Faculty of Food Technology and Biotechnology, accessed 06 May 2024, https://urn.nsk.hr/urn:nbn:hr:159:258410

Jelić A. Rast i produktivnost CHO DP-12 stanica u kemijski definiranim hranjivim medijima [Undergraduate thesis]. Zagreb: University of Zagreb, Faculty of Food Technology and Biotechnology; 2020 [cited 2024 May 06] Available at: https://urn.nsk.hr/urn:nbn:hr:159:258410

A. Jelić, "Rast i produktivnost CHO DP-12 stanica u kemijski definiranim hranjivim medijima", Undergraduate thesis, University of Zagreb, Faculty of Food Technology and Biotechnology, Zagreb, 2020. Available at: https://urn.nsk.hr/urn:nbn:hr:159:258410

Metadata
TitleRast i produktivnost CHO DP-12 stanica u kemijski definiranim hranjivim medijima
Title (english)Growth and Productivity of CHO DP-12 Cells in Chemically Defined Media
AuthorAna Jelić
MentorIgor Slivac (mentor)
GranterUniversity of Zagreb
Faculty of Food Technology and Biotechnology
(Department of Biochemical Engineering)
(Laboratory for Cell Technology, Application and Biotransformations)
Zagreb
Defense date and country2020-09-01, Croatia
Scientific / art field,
discipline and subdiscipline		BIOTECHNICAL SCIENCES
Biotechnology
Abstract
Kulture CHO (eng. Chinese Hamster Ovary) stanica za proizvodnju rekombinantnih proteina zahtijevaju hranjive medije koji potiču rast stanica i sintezu proizvoda. Takvi mediji moraju osigurati visoku vijabilnost i koncentraciju stanica, a ujedno stimulirati sintezu i izlučivanje proizvoda izvan stanice. U ovom radu ispitivan je utjecaj kemijski definiranih medija Dynamis, CD CHO, EX-CELL i PowerCHO na rast i produktivnost stanične linije CHO DP-12 u suspenzijskim kulturama uzgajanim u tikvicama (eng. shake-flask). U svrhu mjerenja koncentracije i vijabilnosti stanica u kulturi korišten je hemocitometar i bojilo tripan-plavo. Koncentracije supstrata (glukoze) i metabolita (laktata) u mediju mjerene su spektrofotometrijski. Procjena produktivnosti staničnih kultura analizirana je prema prisutnosti rekombinantnog humanog monoklonskog protutijela anti-IL8 detektiranog elektroforezom (SDS-PAGE) u medijima u kojima su uzgajane stanice. Rezultati pokazuju da se najbolji rast i najveća produktivnost postižu u mediju Dynamis, dok u medijima CD CHO i EX-CELL nije postignut zadovoljavajući rast stanica što znači da je za uzgoj u njima potrebna stupnjevita prilagodba.
Abstract (english)
The culture of CHO cells (Chinese Hamster Ovary) requires media that support growth and recombinant protein production. Such media must support high viable cell densities while ensuring synthesis of desired products. The aim of this study was to examine the effect of chemically defined media: Dynamis, CD CHO, EX-CELL and PowerCHO, on growth and productivity of CHO DP-12 cell line in suspension culture in shake-flasks. Cell concentration and viability were determined with hemocytometer and trypan blue. Spectrophotometry was used for measuring glucose and lactate concentrations in media. Production of recombinant human anti-IL8 monoclonal antibody was evaluated using SDS-PAGE. The study showed that the best growth and highest productivity were achieved in Dynamis medium. CD CHO and EX-CELL media did not result in satisfactory cell growth indicating that gradual adaptation to these media is required.
Keywords
Keywords (english)
Languagecroatian
URN:NBNurn:nbn:hr:159:258410
Study programmeTitle: Biotechnology
Study programme type: university
Study level: undergraduate
Academic / professional title: sveučilišni/a prvostupnik/prvostupnica (baccalaureus/baccalaurea) inženjer/inženjerka biotehnologije (sveučilišni/a prvostupnik/prvostupnica (baccalaureus/baccalaurea) inženjer/inženjerka biotehnologije)
Type of resourceText
File originBorn digital
Access conditionsOpen access
Terms of useLicence Attribution-NoDerivatives 4.0 International (CC BY-ND 4.0) icon
Created on2020-11-03 13:08:26