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undergraduate thesis
Utjecaj katalitičkih aktivnosti podjedinice RNA egzosoma Dis3 na vijabilnost stanica i stabilnost stanične stijenke kvasca Saccharomyces cerevisiae
Zagreb: University of Zagreb, Faculty of Food Technology and Biotechnology, 2020. urn:nbn:hr:159:897244
Štrbac, Lucija
University of Zagreb
Faculty of Food Technology and Biotechnology
Department of Chemistry and Biochemistry
Laboratory for Biochemistry

Institutional repository: Repository of the Faculty of Food Technology and Biotechnology

Cite this document

Štrbac, L. (2020). Utjecaj katalitičkih aktivnosti podjedinice RNA egzosoma Dis3 na vijabilnost stanica i stabilnost stanične stijenke kvasca Saccharomyces cerevisiae (Undergraduate thesis). Zagreb: University of Zagreb, Faculty of Food Technology and Biotechnology. Retrieved from https://urn.nsk.hr/urn:nbn:hr:159:897244

Štrbac, Lucija. "Utjecaj katalitičkih aktivnosti podjedinice RNA egzosoma Dis3 na vijabilnost stanica i stabilnost stanične stijenke kvasca Saccharomyces cerevisiae." Undergraduate thesis, University of Zagreb, Faculty of Food Technology and Biotechnology, 2020. https://urn.nsk.hr/urn:nbn:hr:159:897244

Štrbac, Lucija. "Utjecaj katalitičkih aktivnosti podjedinice RNA egzosoma Dis3 na vijabilnost stanica i stabilnost stanične stijenke kvasca Saccharomyces cerevisiae." Undergraduate thesis, University of Zagreb, Faculty of Food Technology and Biotechnology, 2020. https://urn.nsk.hr/urn:nbn:hr:159:897244

Štrbac, L. (2020). 'Utjecaj katalitičkih aktivnosti podjedinice RNA egzosoma Dis3 na vijabilnost stanica i stabilnost stanične stijenke kvasca Saccharomyces cerevisiae', Undergraduate thesis, University of Zagreb, Faculty of Food Technology and Biotechnology, accessed 12 January 2025, https://urn.nsk.hr/urn:nbn:hr:159:897244

Štrbac L. Utjecaj katalitičkih aktivnosti podjedinice RNA egzosoma Dis3 na vijabilnost stanica i stabilnost stanične stijenke kvasca Saccharomyces cerevisiae [Undergraduate thesis]. Zagreb: University of Zagreb, Faculty of Food Technology and Biotechnology; 2020 [cited 2025 January 12] Available at: https://urn.nsk.hr/urn:nbn:hr:159:897244

L. Štrbac, "Utjecaj katalitičkih aktivnosti podjedinice RNA egzosoma Dis3 na vijabilnost stanica i stabilnost stanične stijenke kvasca Saccharomyces cerevisiae", Undergraduate thesis, University of Zagreb, Faculty of Food Technology and Biotechnology, Zagreb, 2020. Available at: https://urn.nsk.hr/urn:nbn:hr:159:897244

Metadata
TitleUtjecaj katalitičkih aktivnosti podjedinice RNA egzosoma Dis3 na vijabilnost stanica i stabilnost stanične stijenke kvasca Saccharomyces cerevisiae
Title (english)Influence of catalytic activities of RNA exosome subunit Dis3 on cell viability and cell wall stability of yeast Saccharomyces cerevisiae
AuthorLucija Štrbac
MentorIgor Stuparević (mentor)
GranterUniversity of Zagreb
Faculty of Food Technology and Biotechnology
(Department of Chemistry and Biochemistry)
(Laboratory for Biochemistry)
Zagreb
Defense date and country2020-09-01, Croatia
Scientific / art field,
discipline and subdiscipline		BIOTECHNICAL SCIENCES
Biotechnology
Abstract
Eukariotski RNA egzosom je esencijalan i evolucijski očuvani proteinski kompleks koji degradira i procesira RNA molekule u 3'-5' smjeru. Sastoji se od katalitički-inaktivne jezgre koja ulazi u interakciju s dvije ribonukleazne podjedinice, Dis3 i Rrp6, čije specifične uloge nisu u potpunosti razjašnjene. Podjedinica Dis3 esencijalna je za vijabilnost stanica te posjeduje endoribonukleaznu i egzoribonukleaznu aktivnost. Prethodno je pokazano kako inaktivacija egzoribonukleazne domene Dis3 rezultira sporijim rastom i osjetljivosti na povišenu temperaturu. Rezultati ovog rada pokazuju kako je sporiji rast ovog mutanta pri povišenoj temperaturi posljedica oslabljene stanične stijenke, zbog čega u hipoosmolarnom okruženju dolazi do pucanja stanica. U skladu s navedenim, letalnost i aberantnu morfologiju stanica ovog mutanta u potpunosti anulira osmotska stabilizacija hranjive podloge. Osim toga, stanice ovog mutanta osjetljivije su na agense koji oštećuju staničnu stijenku već pri fiziološkoj temperaturi. Destabilizacija stanične stijenke prilikom inaktivacije egzoribonukleazne, ali ne i endoribonukleazne domene Dis3 vjerojatno proizlazi iz poremećaja u regulaciji metabolizma RNA.
Abstract (english)
The eukaryotic RNA exosome is an essential and conserved protein complex that degrades or processes RNA substrates in the 3'-5' direction. It comprises a non-catalytic core that is associated with two ribonucleases, Dis3 and Rrp6, whose specific functions are not fully understood. Dis3 is essential for viability and has both endoribonuclease and exoribonuclease activity. It was previously shown that disruption of Dis3 exoribonuclease activity by a point mutation results in slow growth and temperature sensitivity. Our results show that the growth defect that these mutant cells display at high temperature is due to a weakened cell wall, which leads to bursting of cells under hypoosmolar conditions. In agreement with this, lethality and aberrant morphology of this mutant can be completely rescued by osmotically stabilizing the growth medium. Furthermore, these mutant cells show increased sensitivity to compounds that act as cell wall stressors already at physiological temperature. Cell wall destabilization upon disruption of Dis3 exoribonuclease, but not endoribonuclease activity, is most probably due to dysregulation of RNA metabolism.
Keywords
Keywords (english)
Languagecroatian
URN:NBNurn:nbn:hr:159:897244
Study programmeTitle: Biotechnology
Study programme type: university
Study level: undergraduate
Academic / professional title: sveučilišni/a prvostupnik/prvostupnica (baccalaureus/baccalaurea) inženjer/inženjerka biotehnologije (sveučilišni/a prvostupnik/prvostupnica (baccalaureus/baccalaurea) inženjer/inženjerka biotehnologije)
Type of resourceText
File originBorn digital
Access conditionsOpen access
Terms of useLicence Attribution-NoDerivatives 4.0 International (CC BY-ND 4.0) icon
Created on2020-11-03 14:06:28