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undergraduate thesis
Klonogena analiza nesteroidnih protuupalnih lijekova
Zagreb: University of Zagreb, Faculty of Food Technology and Biotechnology, 2020. urn:nbn:hr:159:090245
Butumović, Lara
University of Zagreb
Faculty of Food Technology and Biotechnology
Department of Biochemical Engineering
Laboratory for Cell Technology, Application and Biotransformations

Institutional repository: Repository of the Faculty of Food Technology and Biotechnology

Cite this document

Butumović, L. (2020). Klonogena analiza nesteroidnih protuupalnih lijekova (Undergraduate thesis). Zagreb: University of Zagreb, Faculty of Food Technology and Biotechnology. Retrieved from https://urn.nsk.hr/urn:nbn:hr:159:090245

Butumović, Lara. "Klonogena analiza nesteroidnih protuupalnih lijekova." Undergraduate thesis, University of Zagreb, Faculty of Food Technology and Biotechnology, 2020. https://urn.nsk.hr/urn:nbn:hr:159:090245

Butumović, Lara. "Klonogena analiza nesteroidnih protuupalnih lijekova." Undergraduate thesis, University of Zagreb, Faculty of Food Technology and Biotechnology, 2020. https://urn.nsk.hr/urn:nbn:hr:159:090245

Butumović, L. (2020). 'Klonogena analiza nesteroidnih protuupalnih lijekova', Undergraduate thesis, University of Zagreb, Faculty of Food Technology and Biotechnology, accessed 05 August 2024, https://urn.nsk.hr/urn:nbn:hr:159:090245

Butumović L. Klonogena analiza nesteroidnih protuupalnih lijekova [Undergraduate thesis]. Zagreb: University of Zagreb, Faculty of Food Technology and Biotechnology; 2020 [cited 2024 August 05] Available at: https://urn.nsk.hr/urn:nbn:hr:159:090245

L. Butumović, "Klonogena analiza nesteroidnih protuupalnih lijekova", Undergraduate thesis, University of Zagreb, Faculty of Food Technology and Biotechnology, Zagreb, 2020. Available at: https://urn.nsk.hr/urn:nbn:hr:159:090245

Metadata
TitleKlonogena analiza nesteroidnih protuupalnih lijekova
Title (english)Clonogenic analysis of non-steroidal anti-inflammatory drugs
AuthorLara Butumović
MentorKristina Radošević (mentor)
GranterUniversity of Zagreb
Faculty of Food Technology and Biotechnology
(Department of Biochemical Engineering)
(Laboratory for Cell Technology, Application and Biotransformations)
Zagreb
Defense date and country2020-09-01, Croatia
Scientific / art field,
discipline and subdiscipline		BIOTECHNICAL SCIENCES
Biotechnology
Abstract
Nesteroidni protuupalni lijekovi (NSAID, eng. non-steroidal anti-inflammatory drugs) u posljednje se vrijeme sve više istražuju zbog niza farmakoloških djelovanja, posebno kao potencijalni antikancerogeni lijekovi. Sposobni su inhibicijom enzima ciklooksigenaze umanjiti ili potpuno zaustaviti proliferaciju stanica, koja može dovesti do razvoja karcinoma. No, njihov mehanizam djelovanja još nije potpuno objašnjen zbog čega je potrebno provoditi dodatna ispitivanja vezana uz sigurnost, učinkovitost i toksičnost, ali i zbog pojave nuspojava uzrokovanih primjenom ovih lijekova. U ovom radu ispitivan je učinak pet različitih nesteroidnih protuupalnih lijekova (ibuprofen, vedaprofen, fluniksin, diklofenak i tolfenamatna kiselina) koristeći se in vitro metodom i kulturom stanica. Određivana je vijabilnost HeLa i HaCaT stanica nakon tretmana navedenim nesteroidnim protuupalnim lijekovima primjenom MTS kolorimetrijske metode i antikancerogena sposobnost NSAID-a primjenom klonogenog testa. Iz dobivenih rezultata može se zaključiti kako navedeni lijekovi posjeduju antiproliferatni učinak na tumorsku staničnu liniju, ali imaju utjecaja i na normalnu staničnu liniju, zbog čega su potrebna daljna istraživanja u tu svrhu. Inhibitorni učinak ovisan je o koncentraciji ispitivanog lijeka.
Abstract (english)
Lately, non-steroidal anti-inflammatory drugs (NSAID) have been more researched due to their pharmacological activity, especially it's potential as anticancer drugs. NSAIDs are capable of inhibiting the cyclooxygenase enzyme, by which they can completely stop the cell proliferation. Cell proliferation is the key process that contributes to development of tumor growth. But, their mechanism of action is not completely explained which raises the need to implement additional safety, efficiency and toxicological researches because of side effects caused by these drugs. In this work, five different non-steroidal anti-inflammatory drugs (ibuprofen, vedaprofen, flunixine, diclofenac and tolfenamatic acid) were tested using the in vitro method and cell culture. The viability of HeLa and HaCaT cells treated by selected NSAIDs was determined by the colorimetric MTS method and the anticancer ability using a clonogenic test. Obtained results indicate that tested drugs possess antiproliferative effect on the tumor cell line, as well as on the normal cell line. Observed inhibitory effect is dose dependent.
Keywords
Keywords (english)
Languagecroatian
URN:NBNurn:nbn:hr:159:090245
Study programmeTitle: Biotechnology
Study programme type: university
Study level: undergraduate
Academic / professional title: sveučilišni/a prvostupnik/prvostupnica (baccalaureus/baccalaurea) inženjer/inženjerka biotehnologije (sveučilišni/a prvostupnik/prvostupnica (baccalaureus/baccalaurea) inženjer/inženjerka biotehnologije)
Type of resourceText
File originBorn digital
Access conditionsOpen access
Terms of useLicence Attribution-NoDerivatives 4.0 International (CC BY-ND 4.0) icon
Created on2020-11-06 11:23:51