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undergraduate thesis
Vezanje peptida na enzim dipeptidil-peptidazu III (DPP III) metodom izotermne titracijske kalorimetrije (ITC)
Zagreb: University of Zagreb, Faculty of Food Technology and Biotechnology, 2020. urn:nbn:hr:159:593995
Čerkez, Iva
University of Zagreb
Faculty of Food Technology and Biotechnology
Department of Chemistry and Biochemistry
Laboratory for Physical Chemistry and Corrosion

Institutional repository: Repository of the Faculty of Food Technology and Biotechnology

Cite this document

Čerkez, I. (2020). Vezanje peptida na enzim dipeptidil-peptidazu III (DPP III) metodom izotermne titracijske kalorimetrije (ITC) (Undergraduate thesis). Zagreb: University of Zagreb, Faculty of Food Technology and Biotechnology. Retrieved from https://urn.nsk.hr/urn:nbn:hr:159:593995

Čerkez, Iva. "Vezanje peptida na enzim dipeptidil-peptidazu III (DPP III) metodom izotermne titracijske kalorimetrije (ITC)." Undergraduate thesis, University of Zagreb, Faculty of Food Technology and Biotechnology, 2020. https://urn.nsk.hr/urn:nbn:hr:159:593995

Čerkez, Iva. "Vezanje peptida na enzim dipeptidil-peptidazu III (DPP III) metodom izotermne titracijske kalorimetrije (ITC)." Undergraduate thesis, University of Zagreb, Faculty of Food Technology and Biotechnology, 2020. https://urn.nsk.hr/urn:nbn:hr:159:593995

Čerkez, I. (2020). 'Vezanje peptida na enzim dipeptidil-peptidazu III (DPP III) metodom izotermne titracijske kalorimetrije (ITC)', Undergraduate thesis, University of Zagreb, Faculty of Food Technology and Biotechnology, accessed 15 July 2024, https://urn.nsk.hr/urn:nbn:hr:159:593995

Čerkez I. Vezanje peptida na enzim dipeptidil-peptidazu III (DPP III) metodom izotermne titracijske kalorimetrije (ITC) [Undergraduate thesis]. Zagreb: University of Zagreb, Faculty of Food Technology and Biotechnology; 2020 [cited 2024 July 15] Available at: https://urn.nsk.hr/urn:nbn:hr:159:593995

I. Čerkez, "Vezanje peptida na enzim dipeptidil-peptidazu III (DPP III) metodom izotermne titracijske kalorimetrije (ITC)", Undergraduate thesis, University of Zagreb, Faculty of Food Technology and Biotechnology, Zagreb, 2020. Available at: https://urn.nsk.hr/urn:nbn:hr:159:593995

Metadata
TitleVezanje peptida na enzim dipeptidil-peptidazu III (DPP III) metodom izotermne titracijske kalorimetrije (ITC)
Title (english)Isothermal titration calorimetry (ITC) studies of the binding of peptides to enzyme dipeptidyl-peptidase III (DPP III)
AuthorIva Čerkez
MentorFilip Šupljika (mentor)
GranterUniversity of Zagreb
Faculty of Food Technology and Biotechnology
(Department of Chemistry and Biochemistry)
(Laboratory for Physical Chemistry and Corrosion)
Zagreb
Defense date and country2020-09-01, Croatia
Scientific / art field,
discipline and subdiscipline		BIOTECHNICAL SCIENCES
Biotechnology
Abstract
Izotermna titracijska kalorimetrija je kalorimetrijska metoda koja služi za određivanje termodinamičkih parametara interakcija u otopini mjerenjem promjene topline tijekom kemijske reakcije. Aminopeptidaza dipeptidil-peptidaza III je enzim s bitnom funkcijom u brojnim fiziološkim procesima u organizmu, poput obrane od oksidativnog stresa i nastanka nekih tumora, a također je i eksperimentalno dokazana njena uloga u završnim fazama razgradnje bjelančevina. U ovom radu je prikazano praćenje vezanja nekih peptida na ovaj enzim metodom izotermne titracijske kalorimetrije, te je promatrano njihovo potencijalno inhibitorsko djelovanje. Zaključak je da krupni arilni supstituent pridonosi inhibitorskom efektu. Dipeptidil-2-naftilamidni supstrati te oligopeptidi spadaju u skupinu dobrih supstrata za ovaj enzim.
Abstract (english)
Isothermal titration calorimetry is a calorimetric method that is used to determine relevant thermodynamic parameters of certain interactions in solution, by measuring heat changes during chemical reactions. Aminopeptidase dipeptidyl-peptidase III is an enzyme carrying important functions in numerous physiological processes in organism. For instance, the enzyme takes part in defense mechanisms regarding oxidative stress as well as in mechanisms that prevent occurrence of certain tumors. Its role in final stages of protein degradation is also experimentally proven. This thesis discusses monitoring of binding of certain peptides on the above mentioned enzyme as well as impacts of binding, such as peptide’s potential inhibitory action. Both processes are monitored through isothermal titration calorimetry. It is concluded that big aryl substituents contribute to the inhibitory effect. Furthermore, dipeptidyl-2-naphthylamides and oligopeptides belong to the group of good substrates for the enzyme discussed in this thesis.
Keywords
Keywords (english)
Languagecroatian
URN:NBNurn:nbn:hr:159:593995
Study programmeTitle: Biotechnology
Study programme type: university
Study level: undergraduate
Academic / professional title: sveučilišni/a prvostupnik/prvostupnica (baccalaureus/baccalaurea) inženjer/inženjerka biotehnologije (sveučilišni/a prvostupnik/prvostupnica (baccalaureus/baccalaurea) inženjer/inženjerka biotehnologije)
Type of resourceText
File originBorn digital
Access conditionsOpen access
Terms of useLicence Attribution-NoDerivatives 4.0 International (CC BY-ND 4.0) icon
Created on2020-11-06 11:50:31