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undergraduate thesis
Izlaganje heterolognih proteina na površini stanica metilotrofnih kvasaca Pichia pastoris i Hansenula polymorpha
Zagreb: University of Zagreb, Faculty of Food Technology and Biotechnology, 2020. urn:nbn:hr:159:869582
Rušanac, Anamarija
University of Zagreb
Faculty of Food Technology and Biotechnology
Department of Chemistry and Biochemistry
Laboratory for Biochemistry

Institutional repository: Repository of the Faculty of Food Technology and Biotechnology

Cite this document

Rušanac, A. (2020). Izlaganje heterolognih proteina na površini stanica metilotrofnih kvasaca Pichia pastoris i Hansenula polymorpha (Undergraduate thesis). Zagreb: University of Zagreb, Faculty of Food Technology and Biotechnology. Retrieved from https://urn.nsk.hr/urn:nbn:hr:159:869582

Rušanac, Anamarija. "Izlaganje heterolognih proteina na površini stanica metilotrofnih kvasaca Pichia pastoris i Hansenula polymorpha." Undergraduate thesis, University of Zagreb, Faculty of Food Technology and Biotechnology, 2020. https://urn.nsk.hr/urn:nbn:hr:159:869582

Rušanac, Anamarija. "Izlaganje heterolognih proteina na površini stanica metilotrofnih kvasaca Pichia pastoris i Hansenula polymorpha." Undergraduate thesis, University of Zagreb, Faculty of Food Technology and Biotechnology, 2020. https://urn.nsk.hr/urn:nbn:hr:159:869582

Rušanac, A. (2020). 'Izlaganje heterolognih proteina na površini stanica metilotrofnih kvasaca Pichia pastoris i Hansenula polymorpha', Undergraduate thesis, University of Zagreb, Faculty of Food Technology and Biotechnology, accessed 30 December 2024, https://urn.nsk.hr/urn:nbn:hr:159:869582

Rušanac A. Izlaganje heterolognih proteina na površini stanica metilotrofnih kvasaca Pichia pastoris i Hansenula polymorpha [Undergraduate thesis]. Zagreb: University of Zagreb, Faculty of Food Technology and Biotechnology; 2020 [cited 2024 December 30] Available at: https://urn.nsk.hr/urn:nbn:hr:159:869582

A. Rušanac, "Izlaganje heterolognih proteina na površini stanica metilotrofnih kvasaca Pichia pastoris i Hansenula polymorpha", Undergraduate thesis, University of Zagreb, Faculty of Food Technology and Biotechnology, Zagreb, 2020. Available at: https://urn.nsk.hr/urn:nbn:hr:159:869582

Metadata
TitleIzlaganje heterolognih proteina na površini stanica metilotrofnih kvasaca Pichia pastoris i Hansenula polymorpha
Title (english)Surface display of heterologous proteins in methylotrophic yeasts Pichia pastoris and Hansenula polymorpha
AuthorAnamarija Rušanac
MentorRenata Teparić (mentor)
GranterUniversity of Zagreb
Faculty of Food Technology and Biotechnology
(Department of Chemistry and Biochemistry)
(Laboratory for Biochemistry)
Zagreb
Defense date and country2020-09-15, Croatia
Scientific / art field,
discipline and subdiscipline		BIOTECHNICAL SCIENCES
Biotechnology
Abstract
U novije vrijeme, veliki interes za heterologne proteine dovodi do potrebe za pronalaskom efikasnih tehnika za njihovu proizvodnju. Tehnika izlaganja heterologih proteina na površini stanične stijenke kvasca je značajan predmet istraživanja u znanosti i industriji. Njezinom primjenom omogućava se istovremena proizvodnja i sekrecija heterolognih proteina te njihova imobilizacija na površini stanice i primijena kvasaca kao staničnih biokatalizatora. Osnovni nedostatak metode je relativno niska koncentracija imobiliziranih proteina na površini stanica. Ta je činjenica glavni razlog sve većeg broja istraživanja u svrhu poboljšanja metode. Nastoje se pronaći optimalni sustavi za izlaganje heterolognih proteina te pogodni domaćini. Uz do sada najviše istraženi kvasac S. cerevisiae, sve veća važnost se pridaje metilotrofnim kvascima. Cilj ovog rada je prikazati nekonvencionalne metilotrofne kvasce, P. pastoris i H. polymorpha, kao pogodne domaćine za proizvodnju heterolognih proteina te njihovo izlaganje na površini stanica.
Abstract (english)
In recent times, the great interest in heterologous proteins has led to the need of finding efficient techniques for their production. Yeast surface display of heterologous proteins has become a significant subject of research in science and industry. Its application enables the simultaneous production and secretion of heterologous proteins, as well as their immobilization on the cell surface and the use of yeast as whole-cell biocatalysts. The main disadvantage of the method is the relatively low concentration of immobilized proteins on the cell surface. This fact is the main reason for the progress of research in order to improve the method. Great effort has been put into finding the optimal systems for surface display of heterologous proteins and finding the suitable host. In addition to the most researched yeast S. cerevisiae so far, increasing importance is given to methylotrophic yeast. The aim of this study is to present non-conventional methylotrophic yeasts, P. pastoris and H. polymorpha, as suitable hosts for the production of heterologous proteins and their display on the cell surface.
Keywords
Keywords (english)
Languagecroatian
URN:NBNurn:nbn:hr:159:869582
Study programmeTitle: Biotechnology
Study programme type: university
Study level: undergraduate
Academic / professional title: sveučilišni/a prvostupnik/prvostupnica (baccalaureus/baccalaurea) inženjer/inženjerka biotehnologije (sveučilišni/a prvostupnik/prvostupnica (baccalaureus/baccalaurea) inženjer/inženjerka biotehnologije)
Type of resourceText
File originBorn digital
Access conditionsOpen access
Terms of useLicence Attribution-NoDerivatives 4.0 International (CC BY-ND 4.0) icon
Created on2020-11-11 12:37:28