prikaz prve stranice dokumenta Heterologna proizvodnja enzima koji razgrađuju bioplastiku pomoću nekonvencionalnog kvasca Yarrowia lipolytica
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master's thesis
Heterologna proizvodnja enzima koji razgrađuju bioplastiku pomoću nekonvencionalnog kvasca Yarrowia lipolytica
Zagreb: University of Zagreb, Faculty of Food Technology and Biotechnology, 2020. urn:nbn:hr:159:465984
Perić, Ana Marija
University of Zagreb
Faculty of Food Technology and Biotechnology
Department of Biochemical Engineering
Laboratory for Biology and Microbial Genetics

Institutional repository: Repository of the Faculty of Food Technology and Biotechnology

Cite this document

Perić, A. M. (2020). Heterologna proizvodnja enzima koji razgrađuju bioplastiku pomoću nekonvencionalnog kvasca Yarrowia lipolytica (Master's thesis). Zagreb: University of Zagreb, Faculty of Food Technology and Biotechnology. Retrieved from https://urn.nsk.hr/urn:nbn:hr:159:465984

Perić, Ana Marija. "Heterologna proizvodnja enzima koji razgrađuju bioplastiku pomoću nekonvencionalnog kvasca Yarrowia lipolytica." Master's thesis, University of Zagreb, Faculty of Food Technology and Biotechnology, 2020. https://urn.nsk.hr/urn:nbn:hr:159:465984

Perić, Ana Marija. "Heterologna proizvodnja enzima koji razgrađuju bioplastiku pomoću nekonvencionalnog kvasca Yarrowia lipolytica." Master's thesis, University of Zagreb, Faculty of Food Technology and Biotechnology, 2020. https://urn.nsk.hr/urn:nbn:hr:159:465984

Perić, A. M. (2020). 'Heterologna proizvodnja enzima koji razgrađuju bioplastiku pomoću nekonvencionalnog kvasca Yarrowia lipolytica', Master's thesis, University of Zagreb, Faculty of Food Technology and Biotechnology, accessed 24 September 2024, https://urn.nsk.hr/urn:nbn:hr:159:465984

Perić AM. Heterologna proizvodnja enzima koji razgrađuju bioplastiku pomoću nekonvencionalnog kvasca Yarrowia lipolytica [Master's thesis]. Zagreb: University of Zagreb, Faculty of Food Technology and Biotechnology; 2020 [cited 2024 September 24] Available at: https://urn.nsk.hr/urn:nbn:hr:159:465984

A. M. Perić, "Heterologna proizvodnja enzima koji razgrađuju bioplastiku pomoću nekonvencionalnog kvasca Yarrowia lipolytica", Master's thesis, University of Zagreb, Faculty of Food Technology and Biotechnology, Zagreb, 2020. Available at: https://urn.nsk.hr/urn:nbn:hr:159:465984

Metadata
TitleHeterologna proizvodnja enzima koji razgrađuju bioplastiku pomoću nekonvencionalnog kvasca Yarrowia lipolytica
Title (english)Heterologous production of enzymes degrading bioplastic by non-conventional yeast Yarrowia lipolytica
AuthorAna Marija Perić
MentorAnamarija Štafa (mentor)
Committee memberIgor Stuparević (član povjerenstva)
GranterUniversity of Zagreb
Faculty of Food Technology and Biotechnology
(Department of Biochemical Engineering)
(Laboratory for Biology and Microbial Genetics)
Zagreb
Defense date and country2020-11-26, Croatia
Scientific / art field,
discipline and subdiscipline		BIOTECHNICAL SCIENCES
Biotechnology
Abstract
Bioplastika se pojavila kao zamjena za konvencionalnu plastiku, a alifatski poliesteri predstavljaju najbitniju skupinu biorazgradivih polimera. Na razgradnju bioplastike utječu i okolišni uvjeti i svojstva samog materijala, a enzimska hidroliza predstavlja obećavajući izbor za poboljšanje stope razgradnje. U ovom radu konstruirani su sojevi kvasca Yarrowia lipolytica koji eksprimiraju enzime koji bi mogli razgrađivati bioplastiku. Konstruirani sojevi eksprimiraju gene za: Fs kutinazu (iz Fusarium solani) i Tr kutinazu (iz Trichoderma resei), PETazu i modificiranu PETazu R280 (iz Ideonella sakaiensis) te nativnu lipazu 2. Sposobnost biorazgradnje analizirana je na krutim hranjivim podlogama koje su sadržavale alifatske poliestere, polikaprolakton (PCL) ili poli(butilen-sukcinat)-ko-(butilenadipat) (PBSA), a konstruirani sojevi mogu koristiti PCL kao jedini izvor ugljika. Na podlogama s PCLom Fs kutinaza i lipaza 2 imale su veću aktivnost na 25°C, a Tr kutinaza na 37°C. Na podlogama s PBSA-om, jedino je Tr kutinaza imala enzimsku aktivnost.
Abstract (english)
Bioplastic has emerged as a substitute for conventional plastic and aliphatic polyesters are the most important group of biodegradable polymers. Bioplastic degradation depends on both environmental conditions and material properties and enzymatic hydrolysis is a promising option to improve the degradation rate. In this thesis, Yarrowia lipolytica strains, expressing selected bioplasticdegrading enzymes, were constructed. Constructed strains expressed: Fs cutinase (from Fusarium solani) and Tr cutinase (from Trichoderma resei), PETase and engineered PETaseR280 (from Ideonella sakaiensis) and native lipase 2. The biodegradation capability was analyzed by performing plate assays on two different aliphatic polyesters, poly(ε-caprolactone) (PCL) and poly(butylene succinate)-co- (butylene adipate) (PBSA) and constructed strains were able to utilize PCL as a sole carbon source. On PCL plates Fs cutinase and lipase 2 had higher activity at 25°C while Tr cutinase displayed higher activity at 37°C. However, only Tr cutinase had enzymatic activity on PBSA plates.
Keywords
Keywords (english)
Languagecroatian
URN:NBNurn:nbn:hr:159:465984
Study programmeTitle: Molecular Biotechnology
Study programme type: university
Study level: graduate
Academic / professional title: magistar/magistra inženjer/inženjerka molekularne biotehnologije (magistar/magistra inženjer/inženjerka molekularne biotehnologije)
Type of resourceText
File originBorn digital
Access conditionsAccess restricted to students and staff of home institution
Terms of useLicence In copyright icon
Created on2020-12-08 13:51:13