prikaz prve stranice dokumenta Regulation of the urokinase-type plasminogen activator system in mammalian cell lines
  Download
PDF 949.34 KB
master's thesis
Regulation of the urokinase-type plasminogen activator system in mammalian cell lines
Zagreb: University of Zagreb, Faculty of Food Technology and Biotechnology, 2020. urn:nbn:hr:159:592804
Marinić, Laura
University of Zagreb
Faculty of Food Technology and Biotechnology
Department of Chemistry and Biochemistry
Laboratory for Biochemistry

Institutional repository: Repository of the Faculty of Food Technology and Biotechnology

Cite this document

Marinić, L. (2020). Regulation of the urokinase-type plasminogen activator system in mammalian cell lines (Master's thesis). Zagreb: University of Zagreb, Faculty of Food Technology and Biotechnology. Retrieved from https://urn.nsk.hr/urn:nbn:hr:159:592804

Marinić, Laura. "Regulation of the urokinase-type plasminogen activator system in mammalian cell lines." Master's thesis, University of Zagreb, Faculty of Food Technology and Biotechnology, 2020. https://urn.nsk.hr/urn:nbn:hr:159:592804

Marinić, Laura. "Regulation of the urokinase-type plasminogen activator system in mammalian cell lines." Master's thesis, University of Zagreb, Faculty of Food Technology and Biotechnology, 2020. https://urn.nsk.hr/urn:nbn:hr:159:592804

Marinić, L. (2020). 'Regulation of the urokinase-type plasminogen activator system in mammalian cell lines', Master's thesis, University of Zagreb, Faculty of Food Technology and Biotechnology, accessed 03 May 2024, https://urn.nsk.hr/urn:nbn:hr:159:592804

Marinić L. Regulation of the urokinase-type plasminogen activator system in mammalian cell lines [Master's thesis]. Zagreb: University of Zagreb, Faculty of Food Technology and Biotechnology; 2020 [cited 2024 May 03] Available at: https://urn.nsk.hr/urn:nbn:hr:159:592804

L. Marinić, "Regulation of the urokinase-type plasminogen activator system in mammalian cell lines", Master's thesis, University of Zagreb, Faculty of Food Technology and Biotechnology, Zagreb, 2020. Available at: https://urn.nsk.hr/urn:nbn:hr:159:592804

Metadata
TitleRegulation of the urokinase-type plasminogen activator system in mammalian cell lines
Title (english)Regulacija sustava urokinaznog plazminogenskog aktivatora u staničnim linijama sisavaca
AuthorLaura Marinić
MentorMaja Matulić (mentor)
GranterUniversity of Zagreb
Faculty of Food Technology and Biotechnology
(Department of Chemistry and Biochemistry)
(Laboratory for Biochemistry)
Zagreb
Defense date and country2020-09-21, Croatia
Scientific / art field,
discipline and subdiscipline		BIOTECHNICAL SCIENCES
Biotechnology
Abstract
The urokinase-type plasminogen activator system consists of urokinase plasminogen activator (uPA), urokinase type plasminogen activator receptor (uPAR) and plasminogen activator inhibitor-1 (PAI-1). The main role of urokinase is the conversion of plasminogen to plasmin, extracellular protease involved in the degradation of extracellular matrix. uPA system has a role in number of physiological processes, as well as in the metastatic processes. The aim of the study was to compare the urokinase activity and its regulation in different cell lines, under basal conditions and after treatment with several chemotherapeutical agents. Glioblastoma A1235 cells increased the uPA activity after treatment with alkylating agent, retinoic acid and sodium salicylate. Breast cancer cells MDA-MB-231 downregulated urokinase activity after sodium salicylate treatment and increased after treatment with retinoic acid, as well as fibroblast cell line. Dynamin inhibitor inhibited the urokinase activity of A1235 and MDA-MB-231 cells. HEK-293 cells did not change uPA activity after different treatments. In conclusion, the uPA system mechanisms and its regulation are cell-type specific.
Abstract (croatian)
Sustav urokinaznoga plazminogenskoga aktivatora sastoji se od urokinaznog plazminogenskog aktivatora (uPA), receptora urokinaznog plazminogenskog aktivatora (uPAR) i inhibitora plazminogenskog aktivatora-1 (PAI-1). Glavna uloga urokinaze je pretvorba plazminogena u plazmin, izvanstanične proteaze uključene u razgradnju izvanstaničnog matriksa. Uz niz fizioloških procesa, uPA sustav djeluje i kao regulator mikro-okruženja tumora te je uključen u procese metastaziranja. Cilj rada bio je usporediti aktivnost urokinaze i njezinu regulaciju u različitim staničnim linijama, pod bazalnim uvjetima i nakon tretmana s nekoliko kemoterapeutika. Stanice glioblastoma A1235 povećale su aktivnost uPA nakon tretmana alkilirajućim agensom, retinoičnom kiselinom i natrijevim salicilatom. Stanice karcinoma dojke MDA-MB-231 smanjile su aktivnost urokinaze nakon tretmana natrijevim salicilatom, a povećale nakon tretmana retinoičnom kiselinom, kao i stanična linija fibroblasta. Inhibitor dinamina inhibirao je urokinaznu aktivnost stanica A1235 i MDA-MB-231. HEK 293 stanice nisu promijenile uPA aktivnost nakon različitih tretmana. Zaključno, mehanizmi uPA sustava i njegova regulacija stanično su specifični.
Keywords
Keywords (english)
Languageenglish
URN:NBNurn:nbn:hr:159:592804
Study programmeTitle: Molecular Biotechnology
Study programme type: university
Study level: graduate
Academic / professional title: magistar/magistra inženjer/inženjerka molekularne biotehnologije (magistar/magistra inženjer/inženjerka molekularne biotehnologije)
Type of resourceText
File originBorn digital
Access conditionsOpen access
Terms of useLicence In copyright icon
Created on2021-01-21 10:34:47