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master's thesis
Obrazac ekspresije proteina HuD u prenatalnoj moždanoj kori čovjeka
Zagreb: University of Zagreb, Faculty of Food Technology and Biotechnology, 2020. urn:nbn:hr:159:625801
Vukoja, Iva
University of Zagreb
Faculty of Food Technology and Biotechnology
Department of Biochemical Engineering
Laboratory for Biology and Microbial Genetics

Institutional repository: Repository of the Faculty of Food Technology and Biotechnology

Cite this document

Vukoja, I. (2020). Obrazac ekspresije proteina HuD u prenatalnoj moždanoj kori čovjeka (Master's thesis). Zagreb: University of Zagreb, Faculty of Food Technology and Biotechnology. Retrieved from https://urn.nsk.hr/urn:nbn:hr:159:625801

Vukoja, Iva. "Obrazac ekspresije proteina HuD u prenatalnoj moždanoj kori čovjeka." Master's thesis, University of Zagreb, Faculty of Food Technology and Biotechnology, 2020. https://urn.nsk.hr/urn:nbn:hr:159:625801

Vukoja, Iva. "Obrazac ekspresije proteina HuD u prenatalnoj moždanoj kori čovjeka." Master's thesis, University of Zagreb, Faculty of Food Technology and Biotechnology, 2020. https://urn.nsk.hr/urn:nbn:hr:159:625801

Vukoja, I. (2020). 'Obrazac ekspresije proteina HuD u prenatalnoj moždanoj kori čovjeka', Master's thesis, University of Zagreb, Faculty of Food Technology and Biotechnology, accessed 23 November 2024, https://urn.nsk.hr/urn:nbn:hr:159:625801

Vukoja I. Obrazac ekspresije proteina HuD u prenatalnoj moždanoj kori čovjeka [Master's thesis]. Zagreb: University of Zagreb, Faculty of Food Technology and Biotechnology; 2020 [cited 2024 November 23] Available at: https://urn.nsk.hr/urn:nbn:hr:159:625801

I. Vukoja, "Obrazac ekspresije proteina HuD u prenatalnoj moždanoj kori čovjeka", Master's thesis, University of Zagreb, Faculty of Food Technology and Biotechnology, Zagreb, 2020. Available at: https://urn.nsk.hr/urn:nbn:hr:159:625801

Metadata
TitleObrazac ekspresije proteina HuD u prenatalnoj moždanoj kori čovjeka
Title (english)Expression pattern of the HuD protein in the cerebral cortex during human prenatal development
AuthorIva Vukoja
MentorReno Hrašćan (mentor)
Committee memberKristina Radošević (član povjerenstva)
GranterUniversity of Zagreb
Faculty of Food Technology and Biotechnology
(Department of Biochemical Engineering)
(Laboratory for Biology and Microbial Genetics)
Zagreb
Defense date and country2020-09-23, Croatia
Scientific / art field,
discipline and subdiscipline		BIOTECHNICAL SCIENCES
Biotechnology
Abstract
Moždana kora čovjeka je odgovorna za percepciju osjetila, motoriku, emocije, te više kognitivne funkcije, poput razmišljanja, govora, pamćenja, itd. Razvoj moždane kore obuhvaća složene histogenetske procese kao što su proliferacija, diferencijacija, migracija neurona, te sinaptogeneza. Stoga prenatalni razvoj moždane kore obuhvaća nastanak privremenih fetalnih zona u stijenci fetalnog telencefalona: ventrikularna zona, subventrikularna zona, intermedijalna zona, subplate zona, kortikalna ploča i marginalna zona. Pravilan razvoj moždane kore uključuje strogo kontrolirane transkripcijske i post-transkripcijske mehanizme, pri čemu je važnu ulogu pokazao RNA-vezujući protein HuD. Iako je mehanizam djelovanja prethodno proučavan, obrazac ekspresije ovog RNA-vezujućeg proteina u svim privremeno fetalnim zonama ostaje nepoznat. Koristeći metode in situ hibridizacije u ovom dipomskom radu, pokazali smo obrazac ekspresije ELAVL4 mRNA između 10. i 21. tjedna nakon začeća u svim fetalnim zonama. Metoda imunofluorescencije je dala uvid u obrazac ekspresije proteina HuD, koji se pokazao najintenzivnije eksprimiranim u subventrikularnoj zoni i kortikalnoj ploči tijekom ranog fetalnog razdoblja, dok je tijekom srednje fetalnog razdoblja došlo do smanjenja ekspresije u svim zonama, osim u kortikalnoj ploči. Rezultati su pokazali dinamičnost u obrascu ekspresije ELAVL4 mRNA, te usporedbom s obrascem ekspresije proteina HuD dovode do zaključka da je translacija ELAVL4 mRNA strogo regulirana tijekom razvoja moždane kore.
Abstract (english)
Human cerebral cortex is responsible for sensory perception, voluntary movement, emotions, cognitive functions such as thinking, speech, memory, etc. Development of cerebral cortex includes complex histgenetic events such as proliferation, differentiation, neuronal migration and synaptogenesis. Therefore, prenatal development of the cerebral cortex includes formation of temporary fetal zones: ventricular zone, subventricular zone, intermediate zone, subplate zone, cortical plate and marginal zone. To develop properly, the cerebral cortex is regulated by post-transcriptional mechanisms of which important role has RNA binding protein HuD. Even though important HuD molecular interactions and mechanism have been previously studied, its expression pattern in fetal zones remains unknown. Here, using in situ hybridization method, we showed expression pattern of ELAVL4 mRNA between 10 and 21 post conceptual week throughout all fetal zones. Utilizing immunofluorescence, gave us an insight in expression pattern of the HuD protein, that showed highest expression in subventricular zone and cortical plate during early fetal period, while expression pattern during mid fetal period was reduced throughtout cerebral wall except in cortical plate. Therefore, our results showed a dinamic expression pattern of ELAVL4 mRNA. Comparison with the expression pattern of HuD protein leads to conclusion that ELAVL4 mRNA translation is strictly regulated during proper development of cerebral cortex
Keywords
Keywords (english)
Languagecroatian
URN:NBNurn:nbn:hr:159:625801
Study programmeTitle: Molecular Biotechnology
Study programme type: university
Study level: graduate
Academic / professional title: magistar/magistra inženjer/inženjerka molekularne biotehnologije (magistar/magistra inženjer/inženjerka molekularne biotehnologije)
Type of resourceText
File originBorn digital
Access conditionsOpen access
Terms of useLicence In copyright icon
Created on2021-01-25 10:01:26