prikaz prve stranice dokumenta Uklanjanje aflatoksina M1 i metala iz mlijeka primjenom bakterija mliječne kiseline i β-glukana
  Download
PDF 3.57 MB
doctoral thesis
Uklanjanje aflatoksina M1 i metala iz mlijeka primjenom bakterija mliječne kiseline i β-glukana
Zagreb: University of Zagreb, Faculty of Food Technology and Biotechnology, 2020. urn:nbn:hr:159:386095
Kuharić, Željka
University of Zagreb
Faculty of Food Technology and Biotechnology
Department of Biochemical Engineering
Laboratory for General Microbiology and Food Microbiology

Institutional repository: Repository of the Faculty of Food Technology and Biotechnology

Cite this document

Kuharić, Ž. (2020). Uklanjanje aflatoksina M1 i metala iz mlijeka primjenom bakterija mliječne kiseline i β-glukana (Doctoral thesis). Zagreb: University of Zagreb, Faculty of Food Technology and Biotechnology. Retrieved from https://urn.nsk.hr/urn:nbn:hr:159:386095

Kuharić, Željka. "Uklanjanje aflatoksina M1 i metala iz mlijeka primjenom bakterija mliječne kiseline i β-glukana." Doctoral thesis, University of Zagreb, Faculty of Food Technology and Biotechnology, 2020. https://urn.nsk.hr/urn:nbn:hr:159:386095

Kuharić, Željka. "Uklanjanje aflatoksina M1 i metala iz mlijeka primjenom bakterija mliječne kiseline i β-glukana." Doctoral thesis, University of Zagreb, Faculty of Food Technology and Biotechnology, 2020. https://urn.nsk.hr/urn:nbn:hr:159:386095

Kuharić, Ž. (2020). 'Uklanjanje aflatoksina M1 i metala iz mlijeka primjenom bakterija mliječne kiseline i β-glukana', Doctoral thesis, University of Zagreb, Faculty of Food Technology and Biotechnology, accessed 30 November 2024, https://urn.nsk.hr/urn:nbn:hr:159:386095

Kuharić Ž. Uklanjanje aflatoksina M1 i metala iz mlijeka primjenom bakterija mliječne kiseline i β-glukana [Doctoral thesis]. Zagreb: University of Zagreb, Faculty of Food Technology and Biotechnology; 2020 [cited 2024 November 30] Available at: https://urn.nsk.hr/urn:nbn:hr:159:386095

Ž. Kuharić, "Uklanjanje aflatoksina M1 i metala iz mlijeka primjenom bakterija mliječne kiseline i β-glukana", Doctoral thesis, University of Zagreb, Faculty of Food Technology and Biotechnology, Zagreb, 2020. Available at: https://urn.nsk.hr/urn:nbn:hr:159:386095

Metadata
TitleUklanjanje aflatoksina M1 i metala iz mlijeka primjenom bakterija mliječne kiseline i β-glukana
Title (english)Removing of aflatoxin M1 and metals from milk using lactic acid bacteria and β-glucan
AuthorŽeljka Kuharić
MentorKsenija Markov (mentor)
Committee memberJadranka Frece (član povjerenstva)
GranterUniversity of Zagreb
Faculty of Food Technology and Biotechnology
(Department of Biochemical Engineering)
(Laboratory for General Microbiology and Food Microbiology)
Zagreb
Defense date and country2020-11-09, Croatia
Scientific / art field,
discipline and subdiscipline		BIOTECHNICAL SCIENCES
Biotechnology
Universal decimal classification
(UDC)		615 - Pharmacology. Therapeutics. Toxicology
Abstract
Većina živežnih namirnica, pa tako i mlijeko i mliječni proizvodi, sadrže potencijalno opasne tvari, poput aflatoksina M1 (AFM1) i metala. Zbog zabrinutosti o toksičnosti ovih kontaminanata od iznimne je važnosti kontrolirati njihovu prisutnost i koncentraciju u hrani. Obzirom na navedeno, cilj ovog rada bio je primjena biofiksatora (bakterije mliječne kiseline (BMK) i β-glukan) i razvoj postupaka detoksifikacije AFM1 i metala iz mlijeka. Rezultati istraživanja su pokazali da različiti sojevi BMK u ovisnosti o parametrima (žive, mrtve ili liofilizirane stanice i vrijeme trajanja pokusa) pokazuju različitu učinkovitost vezanja AFM1 i pojedinih metala i predstavljaju alternativu postojećim metodama detoksifikacije. Nadalje, dokazano je da β-glukan izoliran iz kvasca (0,01% i 0,005%) ima sposobnost vezanja AFM1 i pojedinih metala, a samim time i mogućnost uklanjanja navedenih kontaminanata iz mlijeka. Predloženi postupci (filtracija i centrifugiranje) uklanjanja kompleksa biofiksator-toksikant pokazali su se učinkoviti u laboratorijskim uvjetima.
Abstract (english)
Most food products, including milk and dairy products, contain certain potentially dangerous substances, such as aflatoxin M1 (AFM1) and metals. Due to concerns about the toxicity of these contaminants, it is very important to control their presence and quantity in food. In view of above mentioned, the aim of this work was to apply biofixators (lactic acid bacteria (LAB) and β-glucan) and to develop the detoxification processes of AFM1 and metals from milk. The results of this research have shown that different strains of LAB depending on the parameters (viable, heat-treated or lyophilized cells and the duration of the experiment) show different binding efficiency of AFM1 and individual metals and represent an alternative to existing detoxification methods. Furthermore, it has been shown that β-glucan isolated from yeast (0,01% and 0,005%) has the ability to bind AFM1 and individual metals, and thus the ability to remove these contaminants from milk. The proposed procedures (filtration and centrifugation) for removing the biofixator-toxicant complex have been shown to be effective under laboratory conditions.
Keywords
Keywords (english)
Languagecroatian
URN:NBNurn:nbn:hr:159:386095
Study programmeTitle: Postgraduate PhD Study in Biotechnology and Bioprocess Engineering
Study programme type: university
Study level: postgraduate
Academic / professional title: doktor/doktorica znanosti (doktor/doktorica znanosti)
Type of resourceText
Extent141 str. : 30 cm.
File originBorn digital
Access conditionsOpen access
Terms of useLicence Attribution-NoDerivatives 4.0 International (CC BY-ND 4.0) icon
Created on2021-01-29 12:25:38