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master's thesis
Genotoksični učinak terapijskih doza Δ9-tetrahidrokanabinola i irinotekana na humane stanične linije karcinoma crijeva i jetre
Zagreb: University of Zagreb, Faculty of Food Technology and Biotechnology, 2021. urn:nbn:hr:159:333785
Genc, Ana - Marija
University of Zagreb
Faculty of Food Technology and Biotechnology
Department of Biochemical Engineering
Laboratory for Biology and Microbial Genetics

Institutional repository: Repository of the Faculty of Food Technology and Biotechnology

Cite this document

Genc, A. M. (2021). Genotoksični učinak terapijskih doza Δ9-tetrahidrokanabinola i irinotekana na humane stanične linije karcinoma crijeva i jetre (Master's thesis). Zagreb: University of Zagreb, Faculty of Food Technology and Biotechnology. Retrieved from https://urn.nsk.hr/urn:nbn:hr:159:333785

Genc, Ana Marija. "Genotoksični učinak terapijskih doza Δ9-tetrahidrokanabinola i irinotekana na humane stanične linije karcinoma crijeva i jetre." Master's thesis, University of Zagreb, Faculty of Food Technology and Biotechnology, 2021. https://urn.nsk.hr/urn:nbn:hr:159:333785

Genc, Ana Marija. "Genotoksični učinak terapijskih doza Δ9-tetrahidrokanabinola i irinotekana na humane stanične linije karcinoma crijeva i jetre." Master's thesis, University of Zagreb, Faculty of Food Technology and Biotechnology, 2021. https://urn.nsk.hr/urn:nbn:hr:159:333785

Genc, A. M. (2021). 'Genotoksični učinak terapijskih doza Δ9-tetrahidrokanabinola i irinotekana na humane stanične linije karcinoma crijeva i jetre', Master's thesis, University of Zagreb, Faculty of Food Technology and Biotechnology, accessed 10 September 2024, https://urn.nsk.hr/urn:nbn:hr:159:333785

Genc AM. Genotoksični učinak terapijskih doza Δ9-tetrahidrokanabinola i irinotekana na humane stanične linije karcinoma crijeva i jetre [Master's thesis]. Zagreb: University of Zagreb, Faculty of Food Technology and Biotechnology; 2021 [cited 2024 September 10] Available at: https://urn.nsk.hr/urn:nbn:hr:159:333785

A. M. Genc, "Genotoksični učinak terapijskih doza Δ9-tetrahidrokanabinola i irinotekana na humane stanične linije karcinoma crijeva i jetre", Master's thesis, University of Zagreb, Faculty of Food Technology and Biotechnology, Zagreb, 2021. Available at: https://urn.nsk.hr/urn:nbn:hr:159:333785

Metadata
TitleGenotoksični učinak terapijskih doza Δ9-tetrahidrokanabinola i irinotekana na humane stanične linije karcinoma crijeva i jetre
Title (english)Genotoxic effect of therapeutic doses of Δ9-tetrahydrocannabinol and irinotecan on human colon and liver cancer cell lines
AuthorAna - Marija Genc
MentorKsenija Durgo (mentor)
Committee memberDraženka Komes (predsjednik povjerenstva)
GranterUniversity of Zagreb
Faculty of Food Technology and Biotechnology
(Department of Biochemical Engineering)
(Laboratory for Biology and Microbial Genetics)
Zagreb
Defense date and country2021-01-29, Croatia
Scientific / art field,
discipline and subdiscipline		BIOTECHNICAL SCIENCES
Interdisciplinary Technical Sciences
Abstract
U većini zemalja svijeta, maligna su oboljenja po učestalosti drugi uzrok smrtnosti stanovništva. O samoj vrsti tumora i njegovoj uznapredovalosti ovisi način liječenja, koji najčešće zahtijeva multimodalni pristup. Irinotekan je jedan od najčešće primjenjivanih citostatika za liječenje metastatskog kolorektalnog karcinoma. Δ9-tetrahidrokanabinol (THC) je glavna aktivna komponenta konoplje čija se uporaba u medicinske svrhe aktivno razmatra posljednjih nekoliko desetljeća. Cilj ovog rada je istražiti citotoksičnost, genotoksičnost i prooksidacijski učinak različitih koncentracija irinotekana i THC-a. Kao test sustavi korištene su humana stanična linija hepatocelularnog karcinoma jetre (HepG2) i humana stanična linija adenokarcinoma epitela debelog crijeva (Caco-2) koje su bile izložene djelovanju irinotekana i THC-a tijekom 2, 4, 24 i 48 sati. Dokazano je da irinotekan i THC djeluju citotoksično ovisno o duljini tretmana stanica i primijenjenoj koncentraciji. Najveći prooksidacijski učinak zabilježen je na obje stanične linije nakon 4 sata, dok genotoksično djelovanje ponajviše uvjetuju više koncentracije irinotekana.
Abstract (english)
In most countries, cancer is the second leading cause of death. The type of tumor and the stage of the disease will depend on the exact treatment, which usually involves a combination of therapies. Irinotecan is the key anti-cancer drug for the treatment of metastatic colorectal cancer. Δ9-tetrahydrocannabinol (THC) is the main active compound in the Cannabis plant which has been increasingly used in medicine over the past decades. The aim of this work is to investigate the cytotoxicity, genotoxicity and pro-oxidative effects of different concentrations of irinotecan and THC. As a test system, the human hepatocellular carcinoma cell line (HepG2) and the human colon adenocarcinoma cell line (Caco-2) were exposed to irinotecan and THC during 2, 4, 24, and 48 hours. It has been shown that the cytotoxicity of irinotecan and THC depends on the duration of cell treatments and concentration used. The highest pro-oxidative effect was observed at 4 hours for both cell lines, while genotoxic activity was mostly caused by higher concentrations of irinotecan.
Keywords
Keywords (english)
Languagecroatian
URN:NBNurn:nbn:hr:159:333785
Study programmeTitle: Nutrition
Study programme type: university
Study level: graduate
Academic / professional title: magistar/magistra nutricionizma (magistar/magistra nutricionizma)
Type of resourceText
File originBorn digital
Access conditionsOpen access
Terms of useLicence Attribution-NoDerivatives 4.0 International (CC BY-ND 4.0) icon
Created on2021-03-17 12:28:06