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master's thesis
Uloga klatrina u infekciji adenovirusnim vektorima tipa 5, 26 i 35
Zagreb: University of Zagreb, Faculty of Food Technology and Biotechnology, 2021. urn:nbn:hr:159:844386
Lindić, Petra
University of Zagreb
Faculty of Food Technology and Biotechnology
Department of Biochemical Engineering
Laboratory for Biology and Microbial Genetics

Institutional repository: Repository of the Faculty of Food Technology and Biotechnology

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Lindić, P. (2021). Uloga klatrina u infekciji adenovirusnim vektorima tipa 5, 26 i 35 (Master's thesis). Zagreb: University of Zagreb, Faculty of Food Technology and Biotechnology. Retrieved from https://urn.nsk.hr/urn:nbn:hr:159:844386

Lindić, Petra. "Uloga klatrina u infekciji adenovirusnim vektorima tipa 5, 26 i 35." Master's thesis, University of Zagreb, Faculty of Food Technology and Biotechnology, 2021. https://urn.nsk.hr/urn:nbn:hr:159:844386

Lindić, Petra. "Uloga klatrina u infekciji adenovirusnim vektorima tipa 5, 26 i 35." Master's thesis, University of Zagreb, Faculty of Food Technology and Biotechnology, 2021. https://urn.nsk.hr/urn:nbn:hr:159:844386

Lindić, P. (2021). 'Uloga klatrina u infekciji adenovirusnim vektorima tipa 5, 26 i 35', Master's thesis, University of Zagreb, Faculty of Food Technology and Biotechnology, accessed 22 November 2024, https://urn.nsk.hr/urn:nbn:hr:159:844386

Lindić P. Uloga klatrina u infekciji adenovirusnim vektorima tipa 5, 26 i 35 [Master's thesis]. Zagreb: University of Zagreb, Faculty of Food Technology and Biotechnology; 2021 [cited 2024 November 22] Available at: https://urn.nsk.hr/urn:nbn:hr:159:844386

P. Lindić, "Uloga klatrina u infekciji adenovirusnim vektorima tipa 5, 26 i 35", Master's thesis, University of Zagreb, Faculty of Food Technology and Biotechnology, Zagreb, 2021. Available at: https://urn.nsk.hr/urn:nbn:hr:159:844386

Metadata
TitleUloga klatrina u infekciji adenovirusnim vektorima tipa 5, 26 i 35
Title (english)Role of clatrin expression in infection with adenovirus vectors type 5, 26 and 35
AuthorPetra Lindić
MentorAndreja Ambriović Ristov (mentor)
Committee memberKristina Radošević (član povjerenstva)
GranterUniversity of Zagreb
Faculty of Food Technology and Biotechnology
(Department of Biochemical Engineering)
(Laboratory for Biology and Microbial Genetics)
Zagreb
Defense date and country2021-01-20, Croatia
Scientific / art field,
discipline and subdiscipline		BIOTECHNICAL SCIENCES
Biotechnology
Abstract
Adenovirusni vektori imaju potencijalno translacijsku i komercijalnu vrijednost, a trenutno se istražuju kao vektori za prijenos nukleinskih kiselina i vakcinaciju. Adenovirusni vektor najčešće korišten u kliničkim istraživanjima je adenovirus tip 5 (AdV5) koji je do sada ujedino i najbolje istražen adenovirus. Prema literaturnim podacima, poznato je da je klatrin uključen u endocitozu AdV5 te smo u ovom radu smanjenjem ekspresije klatrina željeli ispitati da li istu ili sličnu ulogu klatrin ima i u uspješnosti transdukcije adenovirusa tip 26 (AdV26) i adenovirusa tip 35 (AdV35), dva niskoseroprevalentna adenovirusa koji su se pokazali kao obećavajući kandidat za razvoj novih vektorskih vakcina. Klatrin je utišan u A549 stanicama te je nakon određivanja uspješnosti transdukcije adenovirusa, pokazano da smanjenjem količine klatrina dolazi do smanjenja uspješnosti transdukcije s AdV5 i povećanja uspješnosti transdukcije s AdV26. Smanjenje ekspresije klatrina nije imalo značajniji utjecaj na uspješnost transdukcije s AdV35. Rezultati dobiveni u ovom istraživanju ukazuju na važnost klatrinom posredovane endocitoze u uspješnosti transdukcije AdV26 što do sada nije bilo poznato iz literature.
Abstract (english)
Adenovirus based vectors have potential translational and commercial values and are currently studied as vectors for DNA transfer and vaccination. The adenovirus vector most commonly used in clinical trials is adenovirus type 5 (AdV5) which is by far the best researched adenovirus. According to the literature, it is known that clathrin is involved in AdV5 endocytosis, and in this study, we wanted to examine whether clathrin plays the same or similar role in the success of adenovirus type 26 (AdV26) and adenovirus type 35 (AdV35) transduction efficiency, two low seroprevalent adenoviruses which proved to be very promising as a vector vaccine. Clathrin was silenced in A549 cells and after determining the success of adenovirus transduction, it was shown that decreasing the amount of clathrin leads to a decreased transduction efficiency with AdV5 and increased transduction efficiency with AdV26. Decreased clathrin expression did not have a significant effect on the transduction efficiency with AdV35. The results obtained in this study indicate the importance of clathrin-mediated endocytosis in the success of AdV26 transduction, which has not been known from the literature so far.
Keywords
Keywords (english)
Languagecroatian
URN:NBNurn:nbn:hr:159:844386
Study programmeTitle: Molecular Biotechnology
Study programme type: university
Study level: graduate
Academic / professional title: magistar/magistra inženjer/inženjerka molekularne biotehnologije (magistar/magistra inženjer/inženjerka molekularne biotehnologije)
Type of resourceText
File originBorn digital
Access conditionsOpen access
Terms of useLicence Attribution-NoDerivatives 4.0 International (CC BY-ND 4.0) icon
Created on2021-03-17 13:26:34