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undergraduate thesis
Međudjelovanje mRNA-vezujućeg proteina Ssd1 i 3'-5' egzoribonukleaze Rrp6 u održavanju stabilnosti stanične stijenke kvasca Saccharomyces cerevisiae
Zagreb: University of Zagreb, Faculty of Food Technology and Biotechnology, 2021. urn:nbn:hr:159:834967
Bekavac, Nikša
University of Zagreb
Faculty of Food Technology and Biotechnology
Department of Chemistry and Biochemistry
Laboratory for Biochemistry

Institutional repository: Repository of the Faculty of Food Technology and Biotechnology

Cite this document

Bekavac, N. (2021). Međudjelovanje mRNA-vezujućeg proteina Ssd1 i 3'-5' egzoribonukleaze Rrp6 u održavanju stabilnosti stanične stijenke kvasca Saccharomyces cerevisiae (Undergraduate thesis). Zagreb: University of Zagreb, Faculty of Food Technology and Biotechnology. Retrieved from https://urn.nsk.hr/urn:nbn:hr:159:834967

Bekavac, Nikša. "Međudjelovanje mRNA-vezujućeg proteina Ssd1 i 3'-5' egzoribonukleaze Rrp6 u održavanju stabilnosti stanične stijenke kvasca Saccharomyces cerevisiae." Undergraduate thesis, University of Zagreb, Faculty of Food Technology and Biotechnology, 2021. https://urn.nsk.hr/urn:nbn:hr:159:834967

Bekavac, Nikša. "Međudjelovanje mRNA-vezujućeg proteina Ssd1 i 3'-5' egzoribonukleaze Rrp6 u održavanju stabilnosti stanične stijenke kvasca Saccharomyces cerevisiae." Undergraduate thesis, University of Zagreb, Faculty of Food Technology and Biotechnology, 2021. https://urn.nsk.hr/urn:nbn:hr:159:834967

Bekavac, N. (2021). 'Međudjelovanje mRNA-vezujućeg proteina Ssd1 i 3'-5' egzoribonukleaze Rrp6 u održavanju stabilnosti stanične stijenke kvasca Saccharomyces cerevisiae', Undergraduate thesis, University of Zagreb, Faculty of Food Technology and Biotechnology, accessed 25 December 2024, https://urn.nsk.hr/urn:nbn:hr:159:834967

Bekavac N. Međudjelovanje mRNA-vezujućeg proteina Ssd1 i 3'-5' egzoribonukleaze Rrp6 u održavanju stabilnosti stanične stijenke kvasca Saccharomyces cerevisiae [Undergraduate thesis]. Zagreb: University of Zagreb, Faculty of Food Technology and Biotechnology; 2021 [cited 2024 December 25] Available at: https://urn.nsk.hr/urn:nbn:hr:159:834967

N. Bekavac, "Međudjelovanje mRNA-vezujućeg proteina Ssd1 i 3'-5' egzoribonukleaze Rrp6 u održavanju stabilnosti stanične stijenke kvasca Saccharomyces cerevisiae", Undergraduate thesis, University of Zagreb, Faculty of Food Technology and Biotechnology, Zagreb, 2021. Available at: https://urn.nsk.hr/urn:nbn:hr:159:834967

Metadata
TitleMeđudjelovanje mRNA-vezujućeg proteina Ssd1 i 3'-5' egzoribonukleaze Rrp6 u održavanju stabilnosti stanične stijenke kvasca Saccharomyces cerevisiae
Title (english)Interplay of mRNA-binding protein Ssd1 and 3'-5' exoribonuclease Rrp6 in yeast cell wall stability maintenance
AuthorNikša Bekavac
MentorIgor Stuparević (mentor)
GranterUniversity of Zagreb
Faculty of Food Technology and Biotechnology
(Department of Chemistry and Biochemistry)
(Laboratory for Biochemistry)
Zagreb
Defense date and country2021-06-17, Croatia
Scientific / art field,
discipline and subdiscipline		BIOTECHNICAL SCIENCES
Biotechnology
Abstract
RNA-vezujući protein Ssd1 regulira ekspresiju gena koji kodiraju za proteine uključene u stabilizaciju stanične stijenke kvasca. U očuvanju integriteta stanične stijenke bitnu ulogu ima i RNA egzosom, kompleks koji procesira i degradira RNA supstrate egzoribonukleaznom aktivnošću katalitičkih podjedinica Dis3 i Rrp6. Stanice mutanata rrp6 pokazuju temperaturnu osjetljivost koja je posljedica kompromitirane stabilnosti stanične stijenke, a ovaj fenotip je najizraženiji kod linije sojeva kvasca W303. Budući da sojevi ove linije nose nefunkcionalnu inačicu alela koji kodira za Ssd1 (ssd1-d), ispitan je utjecaj ekspresije funkcionalnog alela SSD1-V s odgovarajućeg genomskog lokusa i centromernog plazmida u stanicama mutanata W303 rrp6 i dis3 exo-. Ekspresija SSD1-V suprimirala je defekte u rastu i morfologiji stanica ovih mutanata u odnosu na odgovarajuće ssd1-d stanice pri djelovanju povišene temperature i agenasa koji oštećuju staničnu stijenku. Ovi rezultati upućuju da RNA egzosom i Ssd1 djeluju sinergistički pri održavanju integriteta stanične stijenke kvasca.
Abstract (english)
The RNA-binding protein Ssd1 regulates the expression of genes encoding yeast cell wall morphogenesis proteins. Maintenance of yeast cell wall integrity is also mediated by the RNA exosome, a complex which processes and degrades RNA substrates through exoribonuclease activities of its catalytic subunits, Dis3 and Rrp6. Yeast rrp6 mutant cells display a temperature-sensitive phenotype as a result of impaired cell wall stability, which is most pronounced in the W303 genetic background. Since strains of this background harbour a non-functional form of the allele encoding Ssd1 (ssd1-d), in this work we tested the effect of the expression of the active SSD1-V allele from its corresponding genomic locus and from a centromeric plasmid in W303 rrp6 and dis3 exo- mutant cells. Expression of SSD1-V suppressed growth and morphology defects upon exposure to high temperature and cell wall stressors compared with the corresponding ssd1- d cells. These results suggest that RNA exosome and Ssd1 act synergistically during the maintenance of cell wall integrity in yeast.
Keywords
Keywords (english)
Languagecroatian
URN:NBNurn:nbn:hr:159:834967
Study programmeTitle: Biotechnology
Study programme type: university
Study level: undergraduate
Academic / professional title: sveučilišni/a prvostupnik/prvostupnica (baccalaureus/baccalaurea) inženjer/inženjerka biotehnologije (sveučilišni/a prvostupnik/prvostupnica (baccalaureus/baccalaurea) inženjer/inženjerka biotehnologije)
Type of resourceText
File originBorn digital
Access conditionsOpen access
Terms of useLicence Attribution-NoDerivatives 4.0 International (CC BY-ND 4.0) icon
Created on2021-06-18 09:36:11