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undergraduate thesis
Antimikrobno djelovanje bakterijskih sojeva izoliranih iz majčinog mlijeka
Zagreb: University of Zagreb, Faculty of Food Technology and Biotechnology, 2021. urn:nbn:hr:159:684102
Leko, Lucija
University of Zagreb
Faculty of Food Technology and Biotechnology
Department of Biochemical Engineering
Laboratory for Antibiotic, Enzyme, Probiotic and Starter Cultures Technology

Institutional repository: Repository of the Faculty of Food Technology and Biotechnology

Cite this document

Leko, L. (2021). Antimikrobno djelovanje bakterijskih sojeva izoliranih iz majčinog mlijeka (Undergraduate thesis). Zagreb: University of Zagreb, Faculty of Food Technology and Biotechnology. Retrieved from https://urn.nsk.hr/urn:nbn:hr:159:684102

Leko, Lucija. "Antimikrobno djelovanje bakterijskih sojeva izoliranih iz majčinog mlijeka." Undergraduate thesis, University of Zagreb, Faculty of Food Technology and Biotechnology, 2021. https://urn.nsk.hr/urn:nbn:hr:159:684102

Leko, Lucija. "Antimikrobno djelovanje bakterijskih sojeva izoliranih iz majčinog mlijeka." Undergraduate thesis, University of Zagreb, Faculty of Food Technology and Biotechnology, 2021. https://urn.nsk.hr/urn:nbn:hr:159:684102

Leko, L. (2021). 'Antimikrobno djelovanje bakterijskih sojeva izoliranih iz majčinog mlijeka', Undergraduate thesis, University of Zagreb, Faculty of Food Technology and Biotechnology, accessed 19 May 2024, https://urn.nsk.hr/urn:nbn:hr:159:684102

Leko L. Antimikrobno djelovanje bakterijskih sojeva izoliranih iz majčinog mlijeka [Undergraduate thesis]. Zagreb: University of Zagreb, Faculty of Food Technology and Biotechnology; 2021 [cited 2024 May 19] Available at: https://urn.nsk.hr/urn:nbn:hr:159:684102

L. Leko, "Antimikrobno djelovanje bakterijskih sojeva izoliranih iz majčinog mlijeka", Undergraduate thesis, University of Zagreb, Faculty of Food Technology and Biotechnology, Zagreb, 2021. Available at: https://urn.nsk.hr/urn:nbn:hr:159:684102

Metadata
TitleAntimikrobno djelovanje bakterijskih sojeva izoliranih iz majčinog mlijeka
Title (english)Antimicrobial activity of bacterial strains isolated from breast milk
AuthorLucija Leko
MentorJagoda Šušković (mentor)
GranterUniversity of Zagreb
Faculty of Food Technology and Biotechnology
(Department of Biochemical Engineering)
(Laboratory for Antibiotic, Enzyme, Probiotic and Starter Cultures Technology)
Zagreb
Defense date and country2021-07-09, Croatia
Scientific / art field,
discipline and subdiscipline		BIOTECHNICAL SCIENCES
Biotechnology
Abstract
Mnogi sojevi bakterija mliječne kiseline pokazuju antimikrobno djelovanje pa pronalaze potencijal kao starter kulture, biokonzervansi, probiotici ili kao zamjena antibioticima. Cilj ovog rada bio je ispitati antimikrobno djelovanje sojeva Lactobacillus plantarum KR19 i Lactobacillus plantarum MC19 izoliranih iz majčinog mlijeka prema srodnim bakterijama mliječne kiseline i prema patogenim bakterijama te njihovu kompetitivnu ekskluziju prema bakteriji E. coli 3014. Ispitivanje antimikrobnog djelovanja provedeno je trima metodama: metodom s rupama u agaru, metodom s dvostrukim slojem agara i turbidimetrijskom metodom. Rezultati pokazuju snažno inhibicijsko djelovanje oba soja prema patogenim bakterijama. Kompetitivna ekskluzija ispitana je pomoću Caco-2 stanične linije pri čemu su oba soja pokazala uspješnu inhibiciju adhezije E. coli, pri čemu je soj Lactobacillus plantarum MC19 imao jače djelovanje. Dobiveni rezultati ukazuju na potencijal primjene sojeva Lb. plantarum KR19 i Lb. plantarum MC19 kao probiotika.
Abstract (english)
Many strains of lactic acid bacteria exhibit antimicrobial activity, so they have potential as starter cultures, biopreservatives, probiotics or as a substitute for antibiotics. The aim of this research was to examine the antimicrobial activity of Lactobacillus plantarum KR19 and Lactobacillus plantarum MC19, strains isolated from breast milk, against related lactic acid bacteria and against pathogenic bacteria as well as competitive exclusion of E. coli 3014. Antimicrobial activity assay was carried out by three methods: agar well diffusion method, agar spot test method and broth microdilution assay. The results show a strong inhibition effect of both strains towards pathogenic bacteria. Competitive exclusion was tested using Caco-2 cell line, with both strains showing successful inhibition of E. coli adhesion, with the Lactobacillus plantarum MC19 strain having a stronger effect. The obtained results indicate potential for application of Lb. plantarum KR19 and Lb. plantarum MC19 strains as probiotics.
Keywords
Keywords (english)
Languagecroatian
URN:NBNurn:nbn:hr:159:684102
Study programmeTitle: Biotechnology
Study programme type: university
Study level: undergraduate
Academic / professional title: sveučilišni/a prvostupnik/prvostupnica (baccalaureus/baccalaurea) inženjer/inženjerka biotehnologije (sveučilišni/a prvostupnik/prvostupnica (baccalaureus/baccalaurea) inženjer/inženjerka biotehnologije)
Type of resourceText
File originBorn digital
Access conditionsOpen access
Terms of useLicence Attribution-NoDerivatives 4.0 International (CC BY-ND 4.0) icon
Created on2021-07-15 09:53:51