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undergraduate thesis
Određivanje aktivnosti saharoza fosforilaze HPAE-PAD metodom
Zagreb: University of Zagreb, Faculty of Food Technology and Biotechnology, 2021. urn:nbn:hr:159:521159
Krsnik, Antonia
University of Zagreb
Faculty of Food Technology and Biotechnology
Department of Biochemical Engineering
Laboratory for Biochemical Engineering, Industrial Microbiology and Malting and Brewing Technology

Institutional repository: Repository of the Faculty of Food Technology and Biotechnology

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Krsnik, A. (2021). Određivanje aktivnosti saharoza fosforilaze HPAE-PAD metodom (Undergraduate thesis). Zagreb: University of Zagreb, Faculty of Food Technology and Biotechnology. Retrieved from https://urn.nsk.hr/urn:nbn:hr:159:521159

Krsnik, Antonia. "Određivanje aktivnosti saharoza fosforilaze HPAE-PAD metodom." Undergraduate thesis, University of Zagreb, Faculty of Food Technology and Biotechnology, 2021. https://urn.nsk.hr/urn:nbn:hr:159:521159

Krsnik, Antonia. "Određivanje aktivnosti saharoza fosforilaze HPAE-PAD metodom." Undergraduate thesis, University of Zagreb, Faculty of Food Technology and Biotechnology, 2021. https://urn.nsk.hr/urn:nbn:hr:159:521159

Krsnik, A. (2021). 'Određivanje aktivnosti saharoza fosforilaze HPAE-PAD metodom', Undergraduate thesis, University of Zagreb, Faculty of Food Technology and Biotechnology, accessed 22 December 2024, https://urn.nsk.hr/urn:nbn:hr:159:521159

Krsnik A. Određivanje aktivnosti saharoza fosforilaze HPAE-PAD metodom [Undergraduate thesis]. Zagreb: University of Zagreb, Faculty of Food Technology and Biotechnology; 2021 [cited 2024 December 22] Available at: https://urn.nsk.hr/urn:nbn:hr:159:521159

A. Krsnik, "Određivanje aktivnosti saharoza fosforilaze HPAE-PAD metodom", Undergraduate thesis, University of Zagreb, Faculty of Food Technology and Biotechnology, Zagreb, 2021. Available at: https://urn.nsk.hr/urn:nbn:hr:159:521159

Metadata
TitleOdređivanje aktivnosti saharoza fosforilaze HPAE-PAD metodom
Title (english)Determination of activity of sucrose phosphorylase by HPAE-PAD method
AuthorAntonia Krsnik
MentorAnita Slavica (mentor)
GranterUniversity of Zagreb
Faculty of Food Technology and Biotechnology
(Department of Biochemical Engineering)
(Laboratory for Biochemical Engineering, Industrial Microbiology and Malting and Brewing Technology)
Zagreb
Defense date and country2021-07-09, Croatia
Scientific / art field,
discipline and subdiscipline		BIOTECHNICAL SCIENCES
Biotechnology
Abstract
Aktivnost enzima može se uspješno popratiti određivanjem koncentracije supstrata i/ili produkata enzimske reakcije i to metodama visoko učinkovite tekućinske kromatografije. Saharoza fosforilaza (EC 2.4.1.7) se koristi u industrijskoj proizvodnji α-glukozilglicerola, koji nastaje regioselektivnom glukozilacijom glicerola. Anionsko-izmjenjivačka kromatografija visoke učinkovitosti sa pulsnom amperometrijskom detekcijom (HPAE-PAD) pokazala se kao metoda pogodna za određivanje koncentracije svih spojeva koji sudjeluju u reakciji koju katalizira saharoza foforilaza, a time i procijenu aktivnosti ovoga enzima. Stoga su u ovome završnome radu objedinjeni ključni parametri HPAE-PAD metode za određivanje koncentracije spojeva od interesa, kao i njena teorijska pozadina, čime je poduprta prikladnost HPAE-PAD metode za određivanje aktivnosti saharoza fosforilaze. Posebna pozornost je posvećena fizikalno-kemijskim svojstvima odabranih ugljikohidrata i industrijski važnih i visoko vrijednih biokemikalija, koji nastaju konverzijom ovih supstrata. Dodatno, pobliže su opisani uvjeti pri kojima bi se odabrana analitička metoda provodila i dati su očekivani rezultati za slučaj kada bi analiza ovom metodom bila provedena.
Abstract (english)
Enzyme activity can be successfully followed by determination of substrates and/or products concentration by high-pressure liquid chromatography methods. Sucrose phosphorylase (EC 2.4.1.7) is employed in industrial manufacturing of α-glucosylglycerol which is produced by regioselective glucosylation of glycerol. High pressure anion-exchange chromatography with pulsed amperometric detection (HPAE-PAD) is a suitable method for concentration determination of all compounds involved in a reaction catalized by the sucrose phospohrylase, and therefrom for estimation of the enzyme activity. Therefore, in this graduate thesis all key parameters of the HPAE-PAD method for concentration determination of all given compounds were comprised, as well as a theoretical background of the method. In this way suitability of the HPAE-PAD method for sucrose phospohrylase activity estimation was supported. Special attention was paid to physical and chemical properties of selected carbohydrates and industrially important high-added value biochemicals obtained by conversion of those substrates. In addition, analytical conditions and expected results of predicted analysis by the HPAE-PAD method were described in many details.
Keywords
Keywords (english)
Languagecroatian
URN:NBNurn:nbn:hr:159:521159
Study programmeTitle: Biotechnology
Study programme type: university
Study level: undergraduate
Academic / professional title: sveučilišni/a prvostupnik/prvostupnica (baccalaureus/baccalaurea) inženjer/inženjerka biotehnologije (sveučilišni/a prvostupnik/prvostupnica (baccalaureus/baccalaurea) inženjer/inženjerka biotehnologije)
Type of resourceText
File originBorn digital
Access conditionsOpen access
Terms of useLicence Attribution-NoDerivatives 4.0 International (CC BY-ND 4.0) icon
Created on2021-07-23 11:42:27