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undergraduate thesis
Detekcija bakterija u hemolimfi rakova lančanom reakcijom polimerazom
Zagreb: University of Zagreb, Faculty of Food Technology and Biotechnology, 2021. urn:nbn:hr:159:795871
Doskočil, Bruno
University of Zagreb
Faculty of Food Technology and Biotechnology
Department of Biochemical Engineering
Laboratory for Biology and Microbial Genetics

Institutional repository: Repository of the Faculty of Food Technology and Biotechnology

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Doskočil, B. (2021). Detekcija bakterija u hemolimfi rakova lančanom reakcijom polimerazom (Undergraduate thesis). Zagreb: University of Zagreb, Faculty of Food Technology and Biotechnology. Retrieved from https://urn.nsk.hr/urn:nbn:hr:159:795871

Doskočil, Bruno. "Detekcija bakterija u hemolimfi rakova lančanom reakcijom polimerazom." Undergraduate thesis, University of Zagreb, Faculty of Food Technology and Biotechnology, 2021. https://urn.nsk.hr/urn:nbn:hr:159:795871

Doskočil, Bruno. "Detekcija bakterija u hemolimfi rakova lančanom reakcijom polimerazom." Undergraduate thesis, University of Zagreb, Faculty of Food Technology and Biotechnology, 2021. https://urn.nsk.hr/urn:nbn:hr:159:795871

Doskočil, B. (2021). 'Detekcija bakterija u hemolimfi rakova lančanom reakcijom polimerazom', Undergraduate thesis, University of Zagreb, Faculty of Food Technology and Biotechnology, accessed 03 October 2024, https://urn.nsk.hr/urn:nbn:hr:159:795871

Doskočil B. Detekcija bakterija u hemolimfi rakova lančanom reakcijom polimerazom [Undergraduate thesis]. Zagreb: University of Zagreb, Faculty of Food Technology and Biotechnology; 2021 [cited 2024 October 03] Available at: https://urn.nsk.hr/urn:nbn:hr:159:795871

B. Doskočil, "Detekcija bakterija u hemolimfi rakova lančanom reakcijom polimerazom", Undergraduate thesis, University of Zagreb, Faculty of Food Technology and Biotechnology, Zagreb, 2021. Available at: https://urn.nsk.hr/urn:nbn:hr:159:795871

Metadata
TitleDetekcija bakterija u hemolimfi rakova lančanom reakcijom polimerazom
Title (english)Detection of bacteria in crayfish hemolymph by polymerase chain reaction
AuthorBruno Doskočil
MentorAna Bielen (mentor)
GranterUniversity of Zagreb
Faculty of Food Technology and Biotechnology
(Department of Biochemical Engineering)
(Laboratory for Biology and Microbial Genetics)
Zagreb
Defense date and country2021-07-15, Croatia
Scientific / art field,
discipline and subdiscipline		BIOTECHNICAL SCIENCES
Nutrition
Abstract
Iako su deseteronožni rakovi ključne vrste slatkovodnih ekosustava, brojni aspekti njihove biologije su slabo istraženi, uključujući bakterijske zajednice prirodno prisutne u hemolimfi. Cilj ovog rada bio je po prvi puta pokušati detektirati bakterije u hemolimfi zdravih jedinki uskoškarog raka Pontastacus leptodactylus (zavičajna vrsta) i signalnog raka Pacifastacus leniusculus (invazivna vrsta) lančanom reakcijom polimerazom (engl. polymerase chain reaction, PCR). Na nekoliko lokacija na rijeci Korani uhvaćene su 34 jedinke uskoškarog i 69 jedinki signalnog raka. Nakon izolacije ukupne DNA iz hemolimfe, provedena je amplifikacija gena koji kodira za bakterijsku 16S rRNA. Bakterije su detektirane u 45 % uzoraka hemolimfe uskoškarog raka te samo u 15,7 % uzoraka signalnog raka. Najveći broj pozitivnih uzoraka obje vrste bio je s lokacije Belajske Poljice.
Abstract (english)
Although crayfish are keystone species of freshwater ecosystems, many aspects of their biology remain understudied, including analyses of bacterial communities naturally occurring in the hemolymph. The objective of this study was to detect, for the first time, bacteria in the hemolymph of healthy narrow-clawed crayfish Pontastacus leptodactylus (native species) and signal crayfish Pacifastacus leniusculus (invasive species) using polymerase chain reaction (PCR). Thirty-four individuals of narrow-clawed crayfish and 69 of the signal crayfish were captured at multiple sites in Korana River. After extraction of total DNA from hemolymph, amplification of the gene encoding bacterial 16S rRNA was performed. Bacteria were detected in 45% of the narrow- clawed crayfish hemolymph samples and in 15.7% of samples from signal crayfish. The largest number of positive samples of both species came from the site Belajske Poljice.
Keywords
Keywords (english)
Languagecroatian
URN:NBNurn:nbn:hr:159:795871
Study programmeTitle: Nutrition
Study programme type: university
Study level: undergraduate
Academic / professional title: sveučilišni/a prvostupnik/prvostupnica (baccalaureus/baccalaurea) nutricionizma (sveučilišni/a prvostupnik/prvostupnica (baccalaureus/baccalaurea) nutricionizma)
Type of resourceText
File originBorn digital
Access conditionsOpen access
Terms of useLicence Attribution-NoDerivatives 4.0 International (CC BY-ND 4.0) icon
Created on2021-07-23 12:06:31