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undergraduate thesis
Biotehnološka proizvodnja rekombinantnih terapijskih proteina s pomoću Escherichia coli
Zagreb: University of Zagreb, Faculty of Food Technology and Biotechnology, 2021. urn:nbn:hr:159:476169
Tomšić, Filip
University of Zagreb
Faculty of Food Technology and Biotechnology
Department of Biochemical Engineering
Laboratory for Antibiotic, Enzyme, Probiotic and Starter Cultures Technology

Institutional repository: Repository of the Faculty of Food Technology and Biotechnology

Cite this document

Tomšić, F. (2021). Biotehnološka proizvodnja rekombinantnih terapijskih proteina s pomoću Escherichia coli (Undergraduate thesis). Zagreb: University of Zagreb, Faculty of Food Technology and Biotechnology. Retrieved from https://urn.nsk.hr/urn:nbn:hr:159:476169

Tomšić, Filip. "Biotehnološka proizvodnja rekombinantnih terapijskih proteina s pomoću Escherichia coli." Undergraduate thesis, University of Zagreb, Faculty of Food Technology and Biotechnology, 2021. https://urn.nsk.hr/urn:nbn:hr:159:476169

Tomšić, Filip. "Biotehnološka proizvodnja rekombinantnih terapijskih proteina s pomoću Escherichia coli." Undergraduate thesis, University of Zagreb, Faculty of Food Technology and Biotechnology, 2021. https://urn.nsk.hr/urn:nbn:hr:159:476169

Tomšić, F. (2021). 'Biotehnološka proizvodnja rekombinantnih terapijskih proteina s pomoću Escherichia coli', Undergraduate thesis, University of Zagreb, Faculty of Food Technology and Biotechnology, accessed 27 August 2024, https://urn.nsk.hr/urn:nbn:hr:159:476169

Tomšić F. Biotehnološka proizvodnja rekombinantnih terapijskih proteina s pomoću Escherichia coli [Undergraduate thesis]. Zagreb: University of Zagreb, Faculty of Food Technology and Biotechnology; 2021 [cited 2024 August 27] Available at: https://urn.nsk.hr/urn:nbn:hr:159:476169

F. Tomšić, "Biotehnološka proizvodnja rekombinantnih terapijskih proteina s pomoću Escherichia coli", Undergraduate thesis, University of Zagreb, Faculty of Food Technology and Biotechnology, Zagreb, 2021. Available at: https://urn.nsk.hr/urn:nbn:hr:159:476169

Metadata
TitleBiotehnološka proizvodnja rekombinantnih terapijskih proteina s pomoću Escherichia coli
Title (english)Biotechnological production of recombinant therapeutic protein by Escherichia coli
AuthorFilip Tomšić
MentorBlaženka Kos (mentor)
GranterUniversity of Zagreb
Faculty of Food Technology and Biotechnology
(Department of Biochemical Engineering)
(Laboratory for Antibiotic, Enzyme, Probiotic and Starter Cultures Technology)
Zagreb
Defense date and country2021-07-15, Croatia
Scientific / art field,
discipline and subdiscipline		BIOTECHNICAL SCIENCES
Biotechnology
Abstract
Escherichia coli jedna je od najpoznatijih i najčešće korištenih mikroorganizama za proizvodnju rekombinantnih terapijskih proteina zahvaljujući temeljitom poznavanju genoma bakterije, velikoj brzini rasta i gustoći stanica, laganoj prilagodljivosti mikroorganizma procesima industrijskih razmjera i sveopćoj ekonomičnosti procesa. U ovom radu opisan je proces proizvodnje rekombinantnih terapijskih proteina, od ekspresije ciljanog proteina u samoj bakteriji, tehnika razbijanja stanica, izolacije i otapanja inkluzijskih tijela do renaturacije proteina i ponovnog smatanja u nativnu konformaciju. Nadalje, na primjeru proizvodnje rekombinantnog terapijskog proteina FGF21 opisane su mogućnosti poboljšanja procesa s ciljem optimizacije prinosa proizvoda uz zadržavanje zadovoljavajuće razine kvalitete ciljanog produkta.
Abstract (english)
Escherichia coli is one of the most famous and most commonly used microorganisms for the production of recombinant therapeutic proteins due to the thorough understanding of its genome, rapid growth rate and high cell density, its scalability and overall process economicity. This paper provides an insight into the process of production of recombinant therapeutic proteins from protein expression in E. coli through methods of cell disruption, isolation, solubilization of inclusion bodies, renaturation and refolding of proteins into their native conformation. Furthermore, methods of process intensification are explained using FGF21 recombinant protein production process as an example.
Keywords
Keywords (english)
Languagecroatian
URN:NBNurn:nbn:hr:159:476169
Study programmeTitle: Biotechnology
Study programme type: university
Study level: undergraduate
Academic / professional title: sveučilišni/a prvostupnik/prvostupnica (baccalaureus/baccalaurea) inženjer/inženjerka biotehnologije (sveučilišni/a prvostupnik/prvostupnica (baccalaureus/baccalaurea) inženjer/inženjerka biotehnologije)
Type of resourceText
File originBorn digital
Access conditionsOpen access
Terms of useLicence Attribution-NoDerivatives 4.0 International (CC BY-ND 4.0) icon
Created on2021-07-28 09:19:55