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undergraduate thesis
Enantioselektivna bioredukcija ketona u niskotemperaturnim eutektičkim otapalima
Zagreb: University of Zagreb, Faculty of Food Technology and Biotechnology, 2021. urn:nbn:hr:159:751288
Lacić, Petra
University of Zagreb
Faculty of Food Technology and Biotechnology
Department of Biochemical Engineering
Laboratory for Cell Technology, Application and Biotransformations

Institutional repository: Repository of the Faculty of Food Technology and Biotechnology

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Lacić, P. (2021). Enantioselektivna bioredukcija ketona u niskotemperaturnim eutektičkim otapalima (Undergraduate thesis). Zagreb: University of Zagreb, Faculty of Food Technology and Biotechnology. Retrieved from https://urn.nsk.hr/urn:nbn:hr:159:751288

Lacić, Petra. "Enantioselektivna bioredukcija ketona u niskotemperaturnim eutektičkim otapalima." Undergraduate thesis, University of Zagreb, Faculty of Food Technology and Biotechnology, 2021. https://urn.nsk.hr/urn:nbn:hr:159:751288

Lacić, Petra. "Enantioselektivna bioredukcija ketona u niskotemperaturnim eutektičkim otapalima." Undergraduate thesis, University of Zagreb, Faculty of Food Technology and Biotechnology, 2021. https://urn.nsk.hr/urn:nbn:hr:159:751288

Lacić, P. (2021). 'Enantioselektivna bioredukcija ketona u niskotemperaturnim eutektičkim otapalima', Undergraduate thesis, University of Zagreb, Faculty of Food Technology and Biotechnology, accessed 28 June 2024, https://urn.nsk.hr/urn:nbn:hr:159:751288

Lacić P. Enantioselektivna bioredukcija ketona u niskotemperaturnim eutektičkim otapalima [Undergraduate thesis]. Zagreb: University of Zagreb, Faculty of Food Technology and Biotechnology; 2021 [cited 2024 June 28] Available at: https://urn.nsk.hr/urn:nbn:hr:159:751288

P. Lacić, "Enantioselektivna bioredukcija ketona u niskotemperaturnim eutektičkim otapalima", Undergraduate thesis, University of Zagreb, Faculty of Food Technology and Biotechnology, Zagreb, 2021. Available at: https://urn.nsk.hr/urn:nbn:hr:159:751288

Metadata
TitleEnantioselektivna bioredukcija ketona u niskotemperaturnim eutektičkim otapalima
Title (english)Enantioselective bioreduction of ketones in natural low temperature deep eutectic solvents
AuthorPetra Lacić
MentorMarina Cvjetko Bubalo (mentor)
GranterUniversity of Zagreb
Faculty of Food Technology and Biotechnology
(Department of Biochemical Engineering)
(Laboratory for Cell Technology, Application and Biotransformations)
Zagreb
Defense date and country2021-09-17, Croatia
Scientific / art field,
discipline and subdiscipline		BIOTECHNICAL SCIENCES
Biotechnology
Abstract
Posljednjih godina nastoji se smanjiti upotreba organskih hlapljivih otapala zbog negativnog utjecaja na okoliš. Takva otapala se pristupom zelene kemije nastoje zamijeniti prirodnom alternativom koja ne škodi ljudskom zdravlju i okolišu. Nova generacija takvih otapala su niskotemperaturna eutektička otapala (engl. Deep Eutectic solvents, DES) koja karakteriziraju jednostavna sinteza, niska hlapljivost, biorazgradivost. U ovom radu provedena je reakcija enantioselektivne redukcije halogeniranog acetofenona katalizirane enzimima pekarskog kvasca Saccharomyces cerevisiae u niskotemperaturnom eutektičkom otapalu kolin klorid:etilen-glikol s različitim masenim udjelima vode. Također, ispitana je i vijabilnost stanica te utjecaj predtretmana stanica kvasca kuglicama na konverziju reakcije i stereoselektivnost biokatalizatora. S obzirom na rezultate konverzije i enantiomerni višak DES su se pokazala kao povoljan izbor za bioredukciju kao i korištenje kvasca kao biokatalizatora u odnosu na pufer.
Abstract (english)
In recent years, efforts have been made to reduce the use of organic volatile solvents due to the negative impact on the environment. With the approach of green chemistry, such solvents are being replaced by a natural alternative that does not harm human health and the environment. A new generation of such solvents are low temperature deep eutectic solvents characterized by simple synthesis, low volatility, biodegradability. In this study, the enantioselective reduction of halogenated acetophenone catalyzed by enzymes of the baker's yeast Saccharomyces cerevisiae in low temperature deep eutectic solvents choline cloride:ethylene-glycol with different water contents was performed. Also, cell viability and the influence of yeast cell pretreatment with beads on the reaction yield and stereoselectivity of biocatalysts were examined. Given the results of utilization and enantiomeric excess DES have been proved to be a favorable choice for bioreduction as well as the use of yeast as a biocatalyst compared to buffer.
Keywords
Keywords (english)
Languagecroatian
URN:NBNurn:nbn:hr:159:751288
Study programmeTitle: Biotechnology
Study programme type: university
Study level: undergraduate
Academic / professional title: sveučilišni/a prvostupnik/prvostupnica (baccalaureus/baccalaurea) inženjer/inženjerka biotehnologije (sveučilišni/a prvostupnik/prvostupnica (baccalaureus/baccalaurea) inženjer/inženjerka biotehnologije)
Type of resourceText
File originBorn digital
Access conditionsOpen access
Terms of useLicence Attribution-NoDerivatives 4.0 International (CC BY-ND 4.0) icon
Created on2021-09-30 10:52:04