prikaz prve stranice dokumenta Genetic manipulations of Serratia marcescens with a goal of industrial application
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master's thesis
Genetic manipulations of Serratia marcescens with a goal of industrial application
Zagreb: University of Zagreb, Faculty of Food Technology and Biotechnology, 2021. urn:nbn:hr:159:274300
Kozarić, Marta
University of Zagreb
Faculty of Food Technology and Biotechnology
Department of Biochemical Engineering
Laboratory for Biochemical Engineering, Industrial Microbiology and Malting and Brewing Technology

Institutional repository: Repository of the Faculty of Food Technology and Biotechnology

Cite this document

Kozarić, M. (2021). Genetic manipulations of Serratia marcescens with a goal of industrial application (Master's thesis). Zagreb: University of Zagreb, Faculty of Food Technology and Biotechnology. Retrieved from https://urn.nsk.hr/urn:nbn:hr:159:274300

Kozarić, Marta. "Genetic manipulations of Serratia marcescens with a goal of industrial application." Master's thesis, University of Zagreb, Faculty of Food Technology and Biotechnology, 2021. https://urn.nsk.hr/urn:nbn:hr:159:274300

Kozarić, Marta. "Genetic manipulations of Serratia marcescens with a goal of industrial application." Master's thesis, University of Zagreb, Faculty of Food Technology and Biotechnology, 2021. https://urn.nsk.hr/urn:nbn:hr:159:274300

Kozarić, M. (2021). 'Genetic manipulations of Serratia marcescens with a goal of industrial application', Master's thesis, University of Zagreb, Faculty of Food Technology and Biotechnology, accessed 26 December 2024, https://urn.nsk.hr/urn:nbn:hr:159:274300

Kozarić M. Genetic manipulations of Serratia marcescens with a goal of industrial application [Master's thesis]. Zagreb: University of Zagreb, Faculty of Food Technology and Biotechnology; 2021 [cited 2024 December 26] Available at: https://urn.nsk.hr/urn:nbn:hr:159:274300

M. Kozarić, "Genetic manipulations of Serratia marcescens with a goal of industrial application", Master's thesis, University of Zagreb, Faculty of Food Technology and Biotechnology, Zagreb, 2021. Available at: https://urn.nsk.hr/urn:nbn:hr:159:274300

Metadata
TitleGenetic manipulations of Serratia marcescens with a goal of industrial application
Title (croatian)Genetičke modifikacije Serratia marcescens s ciljem industrijske primjene
AuthorMarta Kozarić
MentorBožidar Šantek (mentor)
Committee memberAnamarija Štafa (član povjerenstva)
GranterUniversity of Zagreb
Faculty of Food Technology and Biotechnology
(Department of Biochemical Engineering)
(Laboratory for Biochemical Engineering, Industrial Microbiology and Malting and Brewing Technology)
Zagreb
Defense date and country2021-09-01, Croatia
Scientific / art field,
discipline and subdiscipline		BIOTECHNICAL SCIENCES
Biotechnology
Abstract
2,3-butanediol (2,3-BD) is one of the promising bulk chemicals that has a great potential for a variety of industries. Serratia marcescens is a Gram negative bacterium capable of producing specific meso-2,3-BD isoform. The main aim of the thesis was genetic engineering of genes connected to glucose uptake in S. marcescens. As target genes, ptsG gene and man operon were chosen and the method of choice for gene knock-out was CRISPR-Cas9. An efficient DNA transformation method for S. marcescens was established in the laboratory where electroporation showed bigger efficiency comparing to chemical competent process. Potential man operon knocked-out clones, grown on xylose as a carbon source, have been revealed but the further examination is required. PtsG gene knocked- out clones were not obtained.
Abstract (croatian)
2,3-butandiol (2,3-BD) kemikalija je s potencijalnom primjenom u raznim granama industrije. Serratia marcescens je Gram negativna bakterija koja može proizvesti specifičnu mezo-2,3-butandiol izoformu. Glavni cilj rada bio je genetički modificirati gene povezane s transportom glukoze u S. marcescens. Kao ciljane regije odabrani su gen ptsG i man operon , a za inaktivaciju gena izabrana je CRISPR- Cas9 metoda. U laboratoriju je uspostavljena učinkovita metoda genetičke transformacije bakterije S. marcescens, te je elektroporacija pokazala veću efikasnost u usporedbi s kemijski kompetentnim postupkom. U konačnici, dobiveni su klonovi u kojima je došlo do moguće inaktivacije man operona, izrasli na ksilozi kao izvoru ugljika, međutim potrebna su daljnja istraživanja. Klonovi u kojima je došlo do inaktivacije gena ptsG nisu dobiveni.
Keywords
Keywords (english)
Languageenglish
URN:NBNurn:nbn:hr:159:274300
Study programmeTitle: Molecular Biotechnology
Study programme type: university
Study level: graduate
Academic / professional title: magistar/magistra inženjer/inženjerka molekularne biotehnologije (magistar/magistra inženjer/inženjerka molekularne biotehnologije)
Type of resourceText
File originBorn digital
Access conditionsOpen access
Terms of useLicence Attribution-NoDerivatives 4.0 International (CC BY-ND 4.0) icon
Created on2021-10-06 12:33:41