prikaz prve stranice dokumenta Adhezija odabranih probiotičkih bakterija na epitelne stanice karcinoma debelog crijeva
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master's thesis
Adhezija odabranih probiotičkih bakterija na epitelne stanice karcinoma debelog crijeva
Zagreb: University of Zagreb, Faculty of Food Technology and Biotechnology, 2016. urn:nbn:hr:159:318145
Džaferović, Iva
University of Zagreb
Faculty of Food Technology and Biotechnology
Department of Biochemical Engineering
Laboratory for Antibiotic, Enzyme, Probiotic and Starter Cultures Technology

Institutional repository: Repository of the Faculty of Food Technology and Biotechnology

Cite this document

Džaferović, I. (2016). Adhezija odabranih probiotičkih bakterija na epitelne stanice karcinoma debelog crijeva (Master's thesis). Zagreb: University of Zagreb, Faculty of Food Technology and Biotechnology. Retrieved from https://urn.nsk.hr/urn:nbn:hr:159:318145

Džaferović, Iva. "Adhezija odabranih probiotičkih bakterija na epitelne stanice karcinoma debelog crijeva." Master's thesis, University of Zagreb, Faculty of Food Technology and Biotechnology, 2016. https://urn.nsk.hr/urn:nbn:hr:159:318145

Džaferović, Iva. "Adhezija odabranih probiotičkih bakterija na epitelne stanice karcinoma debelog crijeva." Master's thesis, University of Zagreb, Faculty of Food Technology and Biotechnology, 2016. https://urn.nsk.hr/urn:nbn:hr:159:318145

Džaferović, I. (2016). 'Adhezija odabranih probiotičkih bakterija na epitelne stanice karcinoma debelog crijeva', Master's thesis, University of Zagreb, Faculty of Food Technology and Biotechnology, accessed 30 December 2024, https://urn.nsk.hr/urn:nbn:hr:159:318145

Džaferović I. Adhezija odabranih probiotičkih bakterija na epitelne stanice karcinoma debelog crijeva [Master's thesis]. Zagreb: University of Zagreb, Faculty of Food Technology and Biotechnology; 2016 [cited 2024 December 30] Available at: https://urn.nsk.hr/urn:nbn:hr:159:318145

I. Džaferović, "Adhezija odabranih probiotičkih bakterija na epitelne stanice karcinoma debelog crijeva", Master's thesis, University of Zagreb, Faculty of Food Technology and Biotechnology, Zagreb, 2016. Available at: https://urn.nsk.hr/urn:nbn:hr:159:318145

Metadata
TitleAdhezija odabranih probiotičkih bakterija na epitelne stanice karcinoma debelog crijeva
Title (english)Adhesion of selected probiotic bacteria on epithelial cells of colon cancer
AuthorIva Džaferović
MentorKsenija Durgo (mentor)
GranterUniversity of Zagreb
Faculty of Food Technology and Biotechnology
(Department of Biochemical Engineering)
(Laboratory for Antibiotic, Enzyme, Probiotic and Starter Cultures Technology)
Zagreb
Defense date and country2016-02-29, Croatia
Scientific / art field,
discipline and subdiscipline		BIOTECHNICAL SCIENCES
Nutrition
Abstract
Probiotici su živi mikroorganizmi, koji primijenjeni u adekvatnoj količini imaju povoljan učinak na zdravlje domaćina. Prilikom odabira probiotičkih sojeva, jedan od najvažnijih kriterija je svojstvo pojedine bakterije da adhezira i kolonizira gastrointestinalni trakt domaćina. Jedan od ciljeva ovog rada je bio ispitati utječe li prisutnost S-sloja na svojstvo adhezije Lactobacillus brevis ZG1 i Lactobacillus brevis SF15B na Caco-2 epitelne stanice, uz sojeve Lactobacillus helveticus M92 za koji je poznato da ima S-sloj (pozitivna kontrola) i Lactobacillus plantarum D13 za koji je poznato da nema površinski S-sloj (negativna kontrola). Rezultati su pokazali da je prisutnost S-sloja veoma bitan faktor za adheziju na crijevni epitel. Uz adheziju, jedan od zahtjeva za odabir probiotičkih sojeva jest i antimikrobna aktivnost među čije mehanizme ubrajamo i kompetitivnu ekskluziju prema patogenima. Ispitani su spomenuti probiotički sojevi u uvjetima predinkubacije i koinkubacije pri čemu se pokazalo da značajno smanjuju sposobnost adhezije patogena Escherichia coli i Salmonella enterica serovar Typhimurium.
Abstract (english)
Probiotics are live microorganisms which, when administered in adequate amounts, confer a health benefit on the host. In the selection of probiotics, one of the most important criteria is the ability of bacterial adhesion and colonization of the hosts gastrointestinal tract. One of the goals of this study was to determine whether the presence of S-layer has the influence on Lactobacillus brevis ZG1 and Lactobacillus brevis SF15B adhesion on Caco-2 epithelial cells, while Lactobacillus helveticus M92 which has the S-layer was the positive control and Lactobacillus plantarum D13, without S-layer, was the negative control. The results showed that the presence of S-layer is an important factor for the adhesion to the intestinal epithelium. Another requirement for the selection of probiotic strains is the antimicrobial activity among whose mechanisms is also the competitive exclusion to pathogens. Mentioned probiotic strains examined in terms of co-incubation and pre-incubation significantly reduced adhesion of pathogenic Escherichia coli and Salmonella enterica serovar Typhimurium.
Keywords
Keywords (english)
Languagecroatian
URN:NBNurn:nbn:hr:159:318145
Study programmeTitle: Nutrition
Study programme type: university
Study level: graduate
Academic / professional title: magistar/magistra nutricionizma (magistar/magistra nutricionizma)
Type of resourceText
File originBorn digital
Access conditionsAccess restricted to students and staff of home institution
Terms of useLicence In copyright icon
Created on2017-04-27 08:43:07