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master's thesis
Racionalan dizajn niskotemperaturnih eutektičkih otapala za enantioselektivnu bioredukciju halogeniranog acetofenona
Zagreb: University of Zagreb, Faculty of Food Technology and Biotechnology, 2021. urn:nbn:hr:159:011786
Šimunčić, Ida
University of Zagreb
Faculty of Food Technology and Biotechnology
Department of Biochemical Engineering
Laboratory for Cell Technology, Application and Biotransformations

Institutional repository: Repository of the Faculty of Food Technology and Biotechnology

Cite this document

Šimunčić, I. (2021). Racionalan dizajn niskotemperaturnih eutektičkih otapala za enantioselektivnu bioredukciju halogeniranog acetofenona (Master's thesis). Zagreb: University of Zagreb, Faculty of Food Technology and Biotechnology. Retrieved from https://urn.nsk.hr/urn:nbn:hr:159:011786

Šimunčić, Ida. "Racionalan dizajn niskotemperaturnih eutektičkih otapala za enantioselektivnu bioredukciju halogeniranog acetofenona." Master's thesis, University of Zagreb, Faculty of Food Technology and Biotechnology, 2021. https://urn.nsk.hr/urn:nbn:hr:159:011786

Šimunčić, Ida. "Racionalan dizajn niskotemperaturnih eutektičkih otapala za enantioselektivnu bioredukciju halogeniranog acetofenona." Master's thesis, University of Zagreb, Faculty of Food Technology and Biotechnology, 2021. https://urn.nsk.hr/urn:nbn:hr:159:011786

Šimunčić, I. (2021). 'Racionalan dizajn niskotemperaturnih eutektičkih otapala za enantioselektivnu bioredukciju halogeniranog acetofenona', Master's thesis, University of Zagreb, Faculty of Food Technology and Biotechnology, accessed 13 March 2025, https://urn.nsk.hr/urn:nbn:hr:159:011786

Šimunčić I. Racionalan dizajn niskotemperaturnih eutektičkih otapala za enantioselektivnu bioredukciju halogeniranog acetofenona [Master's thesis]. Zagreb: University of Zagreb, Faculty of Food Technology and Biotechnology; 2021 [cited 2025 March 13] Available at: https://urn.nsk.hr/urn:nbn:hr:159:011786

I. Šimunčić, "Racionalan dizajn niskotemperaturnih eutektičkih otapala za enantioselektivnu bioredukciju halogeniranog acetofenona", Master's thesis, University of Zagreb, Faculty of Food Technology and Biotechnology, Zagreb, 2021. Available at: https://urn.nsk.hr/urn:nbn:hr:159:011786

Metadata
TitleRacionalan dizajn niskotemperaturnih eutektičkih otapala za enantioselektivnu bioredukciju halogeniranog acetofenona
Title (english)Rational design of deep eutectic solvents for enantioselective bioreduction of halogenated acetophenone
AuthorIda Šimunčić
MentorMarina Cvjetko Bubalo (mentor)
Committee memberIvana Radojčić Redovniković (član povjerenstva)
GranterUniversity of Zagreb
Faculty of Food Technology and Biotechnology
(Department of Biochemical Engineering)
(Laboratory for Cell Technology, Application and Biotransformations)
Zagreb
Defense date and country2021-09-27, Croatia
Scientific / art field,
discipline and subdiscipline		BIOTECHNICAL SCIENCES
Biotechnology
Abstract
Potreba za dizajniranjem kemijskih, farmaceutskih i biotehničkih proizvoda i procesa koji smanjuju ili potpuno uklanjaju primjenu i stvaranje škodljivih i opasnih tvari s jedinstvenim ciljem zaštite okoliša doveli su do razvoja koncepta zelene kemije koji je sve učestaliji industrijski pristup današnjice. Spomenuti pristup podrazumijeva korištenje alternativnih otapala i ekološki prihvatljivih i obnovljivih sirovina, ali i primjenu alternativnih oblika energije te provođenje organskih reakcija primjenom biokatalizatora. Shodno tome, sve je veći interes za primjenom eutektičkih otapala kao medija za biokemijsku pripravu kiralnih biološki aktivnih komponenti. U ovom je radu provedena reakcija enantioselektivne redukcije halogeniranog acetofenona u kiralni halogenirani alkohol, a spomenuta reakcija katalizirana je kvascem Saccharomyces cerevisiae, inače idealnim biokatalizatorom za industrijsku primjenu. Enantioselektivna redukcija provedena je u tri različita niskotemperaturna eutektička otapala s različitim udjelima vode. Biokatalitička reakcija redukcije u eutektičkom otapalu rezultirala je većim iskorištenjem u otapalu kolin klorid : etilen glikol s 10 %-tnim masenim udjelom vode odnosu na bioredukcije provedene u ostalim otapalima i puferu.
Abstract (english)
Designing non-polluting processes and the need to design chemical, pharmaceutical and biotechnical products and processes that reduce or completely eliminate the use and production of harmful and hazardous substances, with the unique goal of environmental protection, have led to the development of green chemistry, which is an increasingly common industrial approach today. This approach involves the use of alternative solvents and environmentally friendly and renewable raw materials, but also the use of alternative forms of energy and the implementation of organic reactions using biocatalysts. Consequently, there is a growing interest in the use of eutectic solvents as a medium for the biochemical preparation of chiral biologically active components. In this work, the reaction of enantioselective reduction of halogenated acetophenone to chiral halogenated alcohol was performed, and the mentioned reaction was catalyzed by the yeast Saccharomyces cerevisiae, otherwise and ideal biocatalysts for industrial application. Enantioselective reduction was performed in three different deep eutectic solvents with different water contents. The biocatalytic reduction in deep eutectic solvents choline chloride : ethylene glycol with 10 % of water resulted in a higher yield compared to the bioreduction perfomed in the other solvents and buffer.
Keywords
Keywords (english)
Languagecroatian
URN:NBNurn:nbn:hr:159:011786
Study programmeTitle: Molecular Biotechnology
Study programme type: university
Study level: graduate
Academic / professional title: magistar/magistra inženjer/inženjerka molekularne biotehnologije (magistar/magistra inženjer/inženjerka molekularne biotehnologije)
Type of resourceText
File originBorn digital
Access conditionsOpen access
Terms of useLicence Attribution-NoDerivatives 4.0 International (CC BY-ND 4.0) icon
Created on2021-10-18 10:57:55