prikaz prve stranice dokumenta Razvoj sustava za ispitivanje interakcija Mate3 proteina zebrice s okolišnim tvarima
  Download
PDF 2.05 MB
master's thesis
Razvoj sustava za ispitivanje interakcija Mate3 proteina zebrice s okolišnim tvarima
Zagreb: University of Zagreb, Faculty of Food Technology and Biotechnology, 2021. urn:nbn:hr:159:570741
Mišić, Lana
University of Zagreb
Faculty of Food Technology and Biotechnology
Department of Chemistry and Biochemistry
Laboratory for Biochemistry

Institutional repository: Repository of the Faculty of Food Technology and Biotechnology

Cite this document

Mišić, L. (2021). Razvoj sustava za ispitivanje interakcija Mate3 proteina zebrice s okolišnim tvarima (Master's thesis). Zagreb: University of Zagreb, Faculty of Food Technology and Biotechnology. Retrieved from https://urn.nsk.hr/urn:nbn:hr:159:570741

Mišić, Lana. "Razvoj sustava za ispitivanje interakcija Mate3 proteina zebrice s okolišnim tvarima." Master's thesis, University of Zagreb, Faculty of Food Technology and Biotechnology, 2021. https://urn.nsk.hr/urn:nbn:hr:159:570741

Mišić, Lana. "Razvoj sustava za ispitivanje interakcija Mate3 proteina zebrice s okolišnim tvarima." Master's thesis, University of Zagreb, Faculty of Food Technology and Biotechnology, 2021. https://urn.nsk.hr/urn:nbn:hr:159:570741

Mišić, L. (2021). 'Razvoj sustava za ispitivanje interakcija Mate3 proteina zebrice s okolišnim tvarima', Master's thesis, University of Zagreb, Faculty of Food Technology and Biotechnology, accessed 04 May 2024, https://urn.nsk.hr/urn:nbn:hr:159:570741

Mišić L. Razvoj sustava za ispitivanje interakcija Mate3 proteina zebrice s okolišnim tvarima [Master's thesis]. Zagreb: University of Zagreb, Faculty of Food Technology and Biotechnology; 2021 [cited 2024 May 04] Available at: https://urn.nsk.hr/urn:nbn:hr:159:570741

L. Mišić, "Razvoj sustava za ispitivanje interakcija Mate3 proteina zebrice s okolišnim tvarima", Master's thesis, University of Zagreb, Faculty of Food Technology and Biotechnology, Zagreb, 2021. Available at: https://urn.nsk.hr/urn:nbn:hr:159:570741

Metadata
TitleRazvoj sustava za ispitivanje interakcija Mate3 proteina zebrice s okolišnim tvarima
Title (english)Development of an assay for studying interactions of zebrafish Mate3 protein with environmental chemicals
AuthorLana Mišić
MentorIgor Stuparević (mentor)
Committee memberIgor Slivac (član povjerenstva)
GranterUniversity of Zagreb
Faculty of Food Technology and Biotechnology
(Department of Chemistry and Biochemistry)
(Laboratory for Biochemistry)
Zagreb
Defense date and country2021-09-24, Croatia
Scientific / art field,
discipline and subdiscipline		BIOTECHNICAL SCIENCES
Biotechnology
Abstract
Ubrzani rast populacije i tehnološki napredak rezultiraju sve većom izloženošću organizama okolišnim ksenobioticima. Nakon početne apsorpcije i distribucije u organizmu, slijedi detoksikacija i izlučivanje ksenobiotika iz organizma, najčešće putem urina ili žuči. U procesu izlučivanja, važnu ulogu imaju MATE sekundarni transporteri, prisutni u svim carstvima živog svijeta, koji kataliziraju elektroneutralan antiport protona i organskih kationa. Uspješno smo, pomoću Flp-In rekombinantnog sustava, razvili stabilnu HEK293T staničnu liniju sa prekomjerno eksprimiranim Mate3 transportnim proteinom zebrice (Danio rerio) koja se smatra jednim od najvažnijih modelnih organizama u ekotoksikološkim studijama. Uspješno smo optimirali visoko procesivan transportni sustav za ispitivanje interakcija Mate3 proteina s okolišnim tvarima, što će omogućiti detaljnu karakterizaciju uloge Mate3 proteina u detoksikacijskim procesima zebrice.
Abstract (english)
Rapid growth of populations and technological advancement has resulted in growing exposure of organisms to the innumerable environmental xenobiotics. After the initial absorption and distribution throughout the organism, xenobiotics are detoxified and excreted outside of the organism, mainly by urine and bile. In the excretion process, the secondary MATE transporters, found in all kingdoms of life, bear a significant role in electroneutral H+/organic cation antiport. By using Flp-In recombinant system, we have successfully developed a stable HEK293T cell line with overexpressed Mate3 transport protein found in zebrafish (Danio rerio), that is considered one of the most important model organisms in ecotoxicological studies. Also, we have successfully optimized highly processive transport assay for identifying interactions between Mate3 protein and environmental substances, which will allow detailed characterization of the Mate3 role in detoxification processes in zebrafish.
Keywords
Keywords (english)
Languagecroatian
URN:NBNurn:nbn:hr:159:570741
Study programmeTitle: Molecular Biotechnology
Study programme type: university
Study level: graduate
Academic / professional title: magistar/magistra inženjer/inženjerka molekularne biotehnologije (magistar/magistra inženjer/inženjerka molekularne biotehnologije)
Type of resourceText
File originBorn digital
Access conditionsOpen access
Terms of useLicence Attribution-NoDerivatives 4.0 International (CC BY-ND 4.0) icon
Created on2021-10-22 09:28:37