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master's thesis
Konformacijske promjene receptora za epidermalni faktor rasta uzrokovane vezanjem različitih liganada
Zagreb: University of Zagreb, Faculty of Food Technology and Biotechnology, 2021. urn:nbn:hr:159:534702
Jukica, Tea
University of Zagreb
Faculty of Food Technology and Biotechnology
Department of Biochemical Engineering
Laboratory for Cell Technology, Application and Biotransformations

Institutional repository: Repository of the Faculty of Food Technology and Biotechnology

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Jukica, T. (2021). Konformacijske promjene receptora za epidermalni faktor rasta uzrokovane vezanjem različitih liganada (Master's thesis). Zagreb: University of Zagreb, Faculty of Food Technology and Biotechnology. Retrieved from https://urn.nsk.hr/urn:nbn:hr:159:534702

Jukica, Tea. "Konformacijske promjene receptora za epidermalni faktor rasta uzrokovane vezanjem različitih liganada." Master's thesis, University of Zagreb, Faculty of Food Technology and Biotechnology, 2021. https://urn.nsk.hr/urn:nbn:hr:159:534702

Jukica, Tea. "Konformacijske promjene receptora za epidermalni faktor rasta uzrokovane vezanjem različitih liganada." Master's thesis, University of Zagreb, Faculty of Food Technology and Biotechnology, 2021. https://urn.nsk.hr/urn:nbn:hr:159:534702

Jukica, T. (2021). 'Konformacijske promjene receptora za epidermalni faktor rasta uzrokovane vezanjem različitih liganada', Master's thesis, University of Zagreb, Faculty of Food Technology and Biotechnology, accessed 19 September 2024, https://urn.nsk.hr/urn:nbn:hr:159:534702

Jukica T. Konformacijske promjene receptora za epidermalni faktor rasta uzrokovane vezanjem različitih liganada [Master's thesis]. Zagreb: University of Zagreb, Faculty of Food Technology and Biotechnology; 2021 [cited 2024 September 19] Available at: https://urn.nsk.hr/urn:nbn:hr:159:534702

T. Jukica, "Konformacijske promjene receptora za epidermalni faktor rasta uzrokovane vezanjem različitih liganada", Master's thesis, University of Zagreb, Faculty of Food Technology and Biotechnology, Zagreb, 2021. Available at: https://urn.nsk.hr/urn:nbn:hr:159:534702

Metadata
TitleKonformacijske promjene receptora za epidermalni faktor rasta uzrokovane vezanjem različitih liganada
Title (english)Conformational changes of epidermal growth factor receptor induced by binding of different ligands
AuthorTea Jukica
MentorIgor Slivac (mentor)
Committee memberJasminka Omerović (član povjerenstva)
GranterUniversity of Zagreb
Faculty of Food Technology and Biotechnology
(Department of Biochemical Engineering)
(Laboratory for Cell Technology, Application and Biotransformations)
Zagreb
Defense date and country2021-09-24, Croatia
Scientific / art field,
discipline and subdiscipline		BIOTECHNICAL SCIENCES
Biotechnology
Abstract
Receptor za epidermalni faktor rasta je jedan u nizu tirozin kinaznih receptora stanične membrane koji vezanjem specifičnih liganada inducira aktivaciju nizvodnih signalnih puteva koji za rezultat imaju proliferaciju diferencijaciju, migraciju i supresiju apoptoze u stanici. Znajući da se strukturna konformacija receptora mijenja ovisno o vezanom ligandu tijekom procesa internalizacije receptora, cilj ovog rada je ispitati razlike u konformaciji kinaznih domena u sklopu homo/heterodimera inducirane dvjema čimbenicima rasta, tj. izvanstaničnim ligandima EGF i TGF-alfa, te sa i bez pristunosti negativnog regulatora iz citosola, Mig6. Ispitivanje se provodilo na transfektiranim CHO-K1 stanicama iz kojih su se izolirani proteini analizirali pomoću Western blot metode. Istraživanje je pokazalo da se kontakti u kinaznim domenama homodimera razlikuju ovisno o vezanom ligandu te da su kontakti u heterodimerima različiti od onih u homodimerima.
Abstract (english)
The epidermal growth factor receptor is one in a series of transmembrane tyrosine kinase receptors that, by binding specific ligands, induce the activation of downstream signaling pathways that result in proliferation, differentiation, migration, and apoptosis suppression of the cell. Knowing that the structural conformation of the receptor changes depending on the bound ligand during the receptor internalization process, the aim of this study is to examine differences in the conformation of kinase domains within homo/heterodimers induced by different growth factors, i.e. extracellular ligands EGF and TGF-alfa, and with or without the presence of a negative regulator from cytosol, Mig6. The assay was performed on transfected CHO-K1 cells from which isolated proteins were analyzed using the Western blot method. The study showed that the contacts in the kinase domains of homodimers differ depending on the bound ligand and that the contacts in heterodimers are different from those in homodimers.
Keywords
Keywords (english)
Languagecroatian
URN:NBNurn:nbn:hr:159:534702
Study programmeTitle: Molecular Biotechnology
Study programme type: university
Study level: graduate
Academic / professional title: magistar/magistra inženjer/inženjerka molekularne biotehnologije (magistar/magistra inženjer/inženjerka molekularne biotehnologije)
Type of resourceText
File originBorn digital
Access conditionsOpen access
Terms of useLicence In copyright icon
Created on2021-10-27 12:22:31